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Distinct mechanisms of signal processing by lamina I spino-parabrachial neurons
Lamina I spino-parabrachial neurons (SPNs) receive peripheral nociceptive input, process it and transmit to the supraspinal centres. Although responses of SPNs to cutaneous receptive field stimulations have been intensively studied, the mechanisms of signal processing in these neurons are poorly und...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6917718/ https://www.ncbi.nlm.nih.gov/pubmed/31848358 http://dx.doi.org/10.1038/s41598-019-55462-7 |
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author | Agashkov, K. Krotov, V. Krasniakova, M. Shevchuk, D. Andrianov, Y. Zabenko, Y. Safronov, B. V. Voitenko, N. Belan, P. |
author_facet | Agashkov, K. Krotov, V. Krasniakova, M. Shevchuk, D. Andrianov, Y. Zabenko, Y. Safronov, B. V. Voitenko, N. Belan, P. |
author_sort | Agashkov, K. |
collection | PubMed |
description | Lamina I spino-parabrachial neurons (SPNs) receive peripheral nociceptive input, process it and transmit to the supraspinal centres. Although responses of SPNs to cutaneous receptive field stimulations have been intensively studied, the mechanisms of signal processing in these neurons are poorly understood. Therefore, we used an ex-vivo spinal cord preparation to examine synaptic and cellular mechanisms determining specific input-output characteristics of the neurons. The vast majority of the SPNs received a few direct nociceptive C-fiber inputs and generated one spike in response to saturating afferent stimulation, thus functioning as simple transducers of painful stimulus. However, 69% of afferent stimulation-induced action potentials in the entire SPN population originated from a small fraction (19%) of high-output neurons. These neurons received a larger number of direct Aδ- and C-fiber inputs, generated intrinsic bursts and efficiently integrated a local network activity via NMDA-receptor-dependent mechanisms. The high-output SPNs amplified and integrated the nociceptive input gradually encoding its intensity into the number of generated spikes. Thus, different mechanisms of signal processing allow lamina I SPNs to play distinct roles in nociception. |
format | Online Article Text |
id | pubmed-6917718 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69177182019-12-18 Distinct mechanisms of signal processing by lamina I spino-parabrachial neurons Agashkov, K. Krotov, V. Krasniakova, M. Shevchuk, D. Andrianov, Y. Zabenko, Y. Safronov, B. V. Voitenko, N. Belan, P. Sci Rep Article Lamina I spino-parabrachial neurons (SPNs) receive peripheral nociceptive input, process it and transmit to the supraspinal centres. Although responses of SPNs to cutaneous receptive field stimulations have been intensively studied, the mechanisms of signal processing in these neurons are poorly understood. Therefore, we used an ex-vivo spinal cord preparation to examine synaptic and cellular mechanisms determining specific input-output characteristics of the neurons. The vast majority of the SPNs received a few direct nociceptive C-fiber inputs and generated one spike in response to saturating afferent stimulation, thus functioning as simple transducers of painful stimulus. However, 69% of afferent stimulation-induced action potentials in the entire SPN population originated from a small fraction (19%) of high-output neurons. These neurons received a larger number of direct Aδ- and C-fiber inputs, generated intrinsic bursts and efficiently integrated a local network activity via NMDA-receptor-dependent mechanisms. The high-output SPNs amplified and integrated the nociceptive input gradually encoding its intensity into the number of generated spikes. Thus, different mechanisms of signal processing allow lamina I SPNs to play distinct roles in nociception. Nature Publishing Group UK 2019-12-17 /pmc/articles/PMC6917718/ /pubmed/31848358 http://dx.doi.org/10.1038/s41598-019-55462-7 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Agashkov, K. Krotov, V. Krasniakova, M. Shevchuk, D. Andrianov, Y. Zabenko, Y. Safronov, B. V. Voitenko, N. Belan, P. Distinct mechanisms of signal processing by lamina I spino-parabrachial neurons |
title | Distinct mechanisms of signal processing by lamina I spino-parabrachial neurons |
title_full | Distinct mechanisms of signal processing by lamina I spino-parabrachial neurons |
title_fullStr | Distinct mechanisms of signal processing by lamina I spino-parabrachial neurons |
title_full_unstemmed | Distinct mechanisms of signal processing by lamina I spino-parabrachial neurons |
title_short | Distinct mechanisms of signal processing by lamina I spino-parabrachial neurons |
title_sort | distinct mechanisms of signal processing by lamina i spino-parabrachial neurons |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6917718/ https://www.ncbi.nlm.nih.gov/pubmed/31848358 http://dx.doi.org/10.1038/s41598-019-55462-7 |
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