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Organization Features of the Mitochondrial Genome of Sunflower (Helianthus annuus L.) with ANN2-Type Male-Sterile Cytoplasm

This study provides insights into the flexibility of the mitochondrial genome in sunflower (Helianthus annuus L.) as well as into the causes of ANN2-type cytoplasmic male sterility (CMS). De novo assembly of the mitochondrial genome of male-sterile HA89(ANN2) sunflower line was performed using high-...

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Autores principales: Makarenko, Maksim S., Usatov, Alexander V., Tatarinova, Tatiana V., Azarin, Kirill V., Logacheva, Maria D., Gavrilova, Vera A., Kornienko, Igor V., Horn, Renate
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918226/
https://www.ncbi.nlm.nih.gov/pubmed/31652744
http://dx.doi.org/10.3390/plants8110439
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author Makarenko, Maksim S.
Usatov, Alexander V.
Tatarinova, Tatiana V.
Azarin, Kirill V.
Logacheva, Maria D.
Gavrilova, Vera A.
Kornienko, Igor V.
Horn, Renate
author_facet Makarenko, Maksim S.
Usatov, Alexander V.
Tatarinova, Tatiana V.
Azarin, Kirill V.
Logacheva, Maria D.
Gavrilova, Vera A.
Kornienko, Igor V.
Horn, Renate
author_sort Makarenko, Maksim S.
collection PubMed
description This study provides insights into the flexibility of the mitochondrial genome in sunflower (Helianthus annuus L.) as well as into the causes of ANN2-type cytoplasmic male sterility (CMS). De novo assembly of the mitochondrial genome of male-sterile HA89(ANN2) sunflower line was performed using high-throughput sequencing technologies. Analysis of CMS ANN2 mitochondrial DNA sequence revealed the following reorganization events: twelve rearrangements, seven insertions, and nine deletions. Comparisons of coding sequences from the male-sterile line with the male-fertile line identified a deletion of orf777 and seven new transcriptionally active open reading frames (ORFs): orf324, orf327, orf345, orf558, orf891, orf933, orf1197. Three of these ORFs represent chimeric genes involving atp6 (orf1197), cox2 (orf558), and nad6 (orf891). In addition, orf558, orf891, orf1197, as well as orf933, encode proteins containing membrane domain(s), making them the most likely candidate genes for CMS development in ANN2. Although the investigated CMS phenotype may be caused by simultaneous action of several candidate genes, we assume that orf1197 plays a major role in developing male sterility in ANN2. Comparative analysis of mitogenome organization in sunflower lines representing different CMS sources also allowed identification of reorganization hot spots in the mitochondrial genome of sunflower.
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spelling pubmed-69182262019-12-24 Organization Features of the Mitochondrial Genome of Sunflower (Helianthus annuus L.) with ANN2-Type Male-Sterile Cytoplasm Makarenko, Maksim S. Usatov, Alexander V. Tatarinova, Tatiana V. Azarin, Kirill V. Logacheva, Maria D. Gavrilova, Vera A. Kornienko, Igor V. Horn, Renate Plants (Basel) Article This study provides insights into the flexibility of the mitochondrial genome in sunflower (Helianthus annuus L.) as well as into the causes of ANN2-type cytoplasmic male sterility (CMS). De novo assembly of the mitochondrial genome of male-sterile HA89(ANN2) sunflower line was performed using high-throughput sequencing technologies. Analysis of CMS ANN2 mitochondrial DNA sequence revealed the following reorganization events: twelve rearrangements, seven insertions, and nine deletions. Comparisons of coding sequences from the male-sterile line with the male-fertile line identified a deletion of orf777 and seven new transcriptionally active open reading frames (ORFs): orf324, orf327, orf345, orf558, orf891, orf933, orf1197. Three of these ORFs represent chimeric genes involving atp6 (orf1197), cox2 (orf558), and nad6 (orf891). In addition, orf558, orf891, orf1197, as well as orf933, encode proteins containing membrane domain(s), making them the most likely candidate genes for CMS development in ANN2. Although the investigated CMS phenotype may be caused by simultaneous action of several candidate genes, we assume that orf1197 plays a major role in developing male sterility in ANN2. Comparative analysis of mitogenome organization in sunflower lines representing different CMS sources also allowed identification of reorganization hot spots in the mitochondrial genome of sunflower. MDPI 2019-10-23 /pmc/articles/PMC6918226/ /pubmed/31652744 http://dx.doi.org/10.3390/plants8110439 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Makarenko, Maksim S.
Usatov, Alexander V.
Tatarinova, Tatiana V.
Azarin, Kirill V.
Logacheva, Maria D.
Gavrilova, Vera A.
Kornienko, Igor V.
Horn, Renate
Organization Features of the Mitochondrial Genome of Sunflower (Helianthus annuus L.) with ANN2-Type Male-Sterile Cytoplasm
title Organization Features of the Mitochondrial Genome of Sunflower (Helianthus annuus L.) with ANN2-Type Male-Sterile Cytoplasm
title_full Organization Features of the Mitochondrial Genome of Sunflower (Helianthus annuus L.) with ANN2-Type Male-Sterile Cytoplasm
title_fullStr Organization Features of the Mitochondrial Genome of Sunflower (Helianthus annuus L.) with ANN2-Type Male-Sterile Cytoplasm
title_full_unstemmed Organization Features of the Mitochondrial Genome of Sunflower (Helianthus annuus L.) with ANN2-Type Male-Sterile Cytoplasm
title_short Organization Features of the Mitochondrial Genome of Sunflower (Helianthus annuus L.) with ANN2-Type Male-Sterile Cytoplasm
title_sort organization features of the mitochondrial genome of sunflower (helianthus annuus l.) with ann2-type male-sterile cytoplasm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918226/
https://www.ncbi.nlm.nih.gov/pubmed/31652744
http://dx.doi.org/10.3390/plants8110439
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