Cargando…

Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway

PURPOSE: To observe the changes of Nogo/NgR and Rho/ROCK signaling pathway-related gene and protein expression in rats with spinal cord injury (SCI) treated with electroacupuncture (EA) and to further investigate the possible mechanism of EA for treating SCI. METHODS: Allen’s method was used to crea...

Descripción completa

Detalles Bibliográficos
Autores principales: Xiao, Wei-Ping, Ding, Li-Li-Qiang, Min, You-Jiang, Yang, Hua-Yuan, Yao, Hai-Hua, Sun, Jie, Zhou, Xuan, Zeng, Xue-Bo, Yu, Wan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918258/
https://www.ncbi.nlm.nih.gov/pubmed/31997879
http://dx.doi.org/10.2147/NDT.S216874
_version_ 1783480549401165824
author Xiao, Wei-Ping
Ding, Li-Li-Qiang
Min, You-Jiang
Yang, Hua-Yuan
Yao, Hai-Hua
Sun, Jie
Zhou, Xuan
Zeng, Xue-Bo
Yu, Wan
author_facet Xiao, Wei-Ping
Ding, Li-Li-Qiang
Min, You-Jiang
Yang, Hua-Yuan
Yao, Hai-Hua
Sun, Jie
Zhou, Xuan
Zeng, Xue-Bo
Yu, Wan
author_sort Xiao, Wei-Ping
collection PubMed
description PURPOSE: To observe the changes of Nogo/NgR and Rho/ROCK signaling pathway-related gene and protein expression in rats with spinal cord injury (SCI) treated with electroacupuncture (EA) and to further investigate the possible mechanism of EA for treating SCI. METHODS: Allen’s method was used to create the SCI rat model. Sixty-four model rats were further subdivided into four subgroups, namely, the SCI model group (SCI), EA treatment group (EA), blocking agent Y27632 treatment group (Y27632) and EA+blocking agent Y27632 treatment group (EA+Y), according to the treatment received. The rats were subjected to EA and/or blocking agent Y27632 treatment. After 14 days, injured spinal cord tissue was extracted for analysis. The mRNA and protein expression levels were determined by real-time fluorescence quantitative PCR and Western blotting, respectively. Cell apoptosis changes in the spinal cord were evaluated by in situ hybridization. Hindlimb motor function in the rats was evaluated by Basso-Beattie-Bresnahan assessment methods. RESULTS: Except for RhoA protein expression, compared with the SCI model group, EA, blocking agent Y27632 and EA+blocking agent Y27632 treatment groups had significantly reduced mRNA and protein expression of Nogo-A, NgR, LINGO-1, RhoA and ROCK II in spinal cord tissues, increased mRNA and protein expression of MLCP, decreased p-MYPT1 protein expression and p-MYPT1/MYPT1 ratio, and caspase3 expression, and improved lower limb movement function after treatment for 14 days (P<0.01 or <0.05). The combination of EA and the blocking agent Y27632 was superior to EA or blocking agent Y27632 treatment alone (P < 0.01 or <0.05). CONCLUSION: EA may have an obvious inhibitory effect on the Nogo/NgR and Rho/ROCK signaling pathway after SCI, thereby reducing the inhibition of axonal growth, which may be a key mechanism of EA treatment for SCI.
format Online
Article
Text
id pubmed-6918258
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Dove
record_format MEDLINE/PubMed
spelling pubmed-69182582020-01-29 Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway Xiao, Wei-Ping Ding, Li-Li-Qiang Min, You-Jiang Yang, Hua-Yuan Yao, Hai-Hua Sun, Jie Zhou, Xuan Zeng, Xue-Bo Yu, Wan Neuropsychiatr Dis Treat Original Research PURPOSE: To observe the changes of Nogo/NgR and Rho/ROCK signaling pathway-related gene and protein expression in rats with spinal cord injury (SCI) treated with electroacupuncture (EA) and to further investigate the possible mechanism of EA for treating SCI. METHODS: Allen’s method was used to create the SCI rat model. Sixty-four model rats were further subdivided into four subgroups, namely, the SCI model group (SCI), EA treatment group (EA), blocking agent Y27632 treatment group (Y27632) and EA+blocking agent Y27632 treatment group (EA+Y), according to the treatment received. The rats were subjected to EA and/or blocking agent Y27632 treatment. After 14 days, injured spinal cord tissue was extracted for analysis. The mRNA and protein expression levels were determined by real-time fluorescence quantitative PCR and Western blotting, respectively. Cell apoptosis changes in the spinal cord were evaluated by in situ hybridization. Hindlimb motor function in the rats was evaluated by Basso-Beattie-Bresnahan assessment methods. RESULTS: Except for RhoA protein expression, compared with the SCI model group, EA, blocking agent Y27632 and EA+blocking agent Y27632 treatment groups had significantly reduced mRNA and protein expression of Nogo-A, NgR, LINGO-1, RhoA and ROCK II in spinal cord tissues, increased mRNA and protein expression of MLCP, decreased p-MYPT1 protein expression and p-MYPT1/MYPT1 ratio, and caspase3 expression, and improved lower limb movement function after treatment for 14 days (P<0.01 or <0.05). The combination of EA and the blocking agent Y27632 was superior to EA or blocking agent Y27632 treatment alone (P < 0.01 or <0.05). CONCLUSION: EA may have an obvious inhibitory effect on the Nogo/NgR and Rho/ROCK signaling pathway after SCI, thereby reducing the inhibition of axonal growth, which may be a key mechanism of EA treatment for SCI. Dove 2019-12-13 /pmc/articles/PMC6918258/ /pubmed/31997879 http://dx.doi.org/10.2147/NDT.S216874 Text en © 2019 Xiao et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Xiao, Wei-Ping
Ding, Li-Li-Qiang
Min, You-Jiang
Yang, Hua-Yuan
Yao, Hai-Hua
Sun, Jie
Zhou, Xuan
Zeng, Xue-Bo
Yu, Wan
Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway
title Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway
title_full Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway
title_fullStr Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway
title_full_unstemmed Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway
title_short Electroacupuncture Promoting Axonal Regeneration in Spinal Cord Injury Rats via Suppression of Nogo/NgR and Rho/ROCK Signaling Pathway
title_sort electroacupuncture promoting axonal regeneration in spinal cord injury rats via suppression of nogo/ngr and rho/rock signaling pathway
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918258/
https://www.ncbi.nlm.nih.gov/pubmed/31997879
http://dx.doi.org/10.2147/NDT.S216874
work_keys_str_mv AT xiaoweiping electroacupuncturepromotingaxonalregenerationinspinalcordinjuryratsviasuppressionofnogongrandrhorocksignalingpathway
AT dingliliqiang electroacupuncturepromotingaxonalregenerationinspinalcordinjuryratsviasuppressionofnogongrandrhorocksignalingpathway
AT minyoujiang electroacupuncturepromotingaxonalregenerationinspinalcordinjuryratsviasuppressionofnogongrandrhorocksignalingpathway
AT yanghuayuan electroacupuncturepromotingaxonalregenerationinspinalcordinjuryratsviasuppressionofnogongrandrhorocksignalingpathway
AT yaohaihua electroacupuncturepromotingaxonalregenerationinspinalcordinjuryratsviasuppressionofnogongrandrhorocksignalingpathway
AT sunjie electroacupuncturepromotingaxonalregenerationinspinalcordinjuryratsviasuppressionofnogongrandrhorocksignalingpathway
AT zhouxuan electroacupuncturepromotingaxonalregenerationinspinalcordinjuryratsviasuppressionofnogongrandrhorocksignalingpathway
AT zengxuebo electroacupuncturepromotingaxonalregenerationinspinalcordinjuryratsviasuppressionofnogongrandrhorocksignalingpathway
AT yuwan electroacupuncturepromotingaxonalregenerationinspinalcordinjuryratsviasuppressionofnogongrandrhorocksignalingpathway