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Two SNP Mutations Turned off Seed Shattering in Rice

Seed shattering is an important agronomic trait in rice domestication. In this study, using a near-isogenic line (NIL-hs1) from Oryza barthii, we found a hybrid seed shattering phenomenon between the NIL-hs1 and its recurrent parent, a japonica variety Yundao 1. The heterozygotes at hybrid shatterin...

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Autores principales: Zhang, Yu, Zhou, Jiawu, Yang, Ying, Elgamal, Walid Hassan, Xu, Peng, Li, Jing, El-Refaee, Yasser Z., Hao, Suding, Tao, Dayun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918406/
https://www.ncbi.nlm.nih.gov/pubmed/31698744
http://dx.doi.org/10.3390/plants8110475
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author Zhang, Yu
Zhou, Jiawu
Yang, Ying
Elgamal, Walid Hassan
Xu, Peng
Li, Jing
El-Refaee, Yasser Z.
Hao, Suding
Tao, Dayun
author_facet Zhang, Yu
Zhou, Jiawu
Yang, Ying
Elgamal, Walid Hassan
Xu, Peng
Li, Jing
El-Refaee, Yasser Z.
Hao, Suding
Tao, Dayun
author_sort Zhang, Yu
collection PubMed
description Seed shattering is an important agronomic trait in rice domestication. In this study, using a near-isogenic line (NIL-hs1) from Oryza barthii, we found a hybrid seed shattering phenomenon between the NIL-hs1 and its recurrent parent, a japonica variety Yundao 1. The heterozygotes at hybrid shattering 1 (HS1) exhibited the shattering phenotype, whereas the homozygotes from both parents conferred the non-shattering. The causal HS1 gene for hybrid shattering was located in the region between SSR marker RM17604 and RM8220 on chromosome 4. Sequence verification indicated that HS1 was identical to SH4, and HS1 controlled the hybrid shattering due to harboring the ancestral haplotype, the G allele at G237T site and C allele at C760T site from each parent. Comparative analysis at SH4 showed that all the accessions containing ancestral haplotype, including 78 wild relatives of rice and 8 African cultivated rice, had the shattering phenotype, whereas all the accessions with either of the homozygous domestic haplotypes at one of the two sites, including 17 wild relatives of rice, 111 African cultivated rice and 65 Asian cultivated rice, showed the non-shattering phenotype. Dominant complementation of the G allele at G237T site and the C allele at C760T site in HS1 led to a hybrid shattering phenotype. These results help to shed light on the nature of seed shattering in rice during domestication and improve the moderate shattering varieties adapted to mechanized harvest.
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spelling pubmed-69184062019-12-24 Two SNP Mutations Turned off Seed Shattering in Rice Zhang, Yu Zhou, Jiawu Yang, Ying Elgamal, Walid Hassan Xu, Peng Li, Jing El-Refaee, Yasser Z. Hao, Suding Tao, Dayun Plants (Basel) Article Seed shattering is an important agronomic trait in rice domestication. In this study, using a near-isogenic line (NIL-hs1) from Oryza barthii, we found a hybrid seed shattering phenomenon between the NIL-hs1 and its recurrent parent, a japonica variety Yundao 1. The heterozygotes at hybrid shattering 1 (HS1) exhibited the shattering phenotype, whereas the homozygotes from both parents conferred the non-shattering. The causal HS1 gene for hybrid shattering was located in the region between SSR marker RM17604 and RM8220 on chromosome 4. Sequence verification indicated that HS1 was identical to SH4, and HS1 controlled the hybrid shattering due to harboring the ancestral haplotype, the G allele at G237T site and C allele at C760T site from each parent. Comparative analysis at SH4 showed that all the accessions containing ancestral haplotype, including 78 wild relatives of rice and 8 African cultivated rice, had the shattering phenotype, whereas all the accessions with either of the homozygous domestic haplotypes at one of the two sites, including 17 wild relatives of rice, 111 African cultivated rice and 65 Asian cultivated rice, showed the non-shattering phenotype. Dominant complementation of the G allele at G237T site and the C allele at C760T site in HS1 led to a hybrid shattering phenotype. These results help to shed light on the nature of seed shattering in rice during domestication and improve the moderate shattering varieties adapted to mechanized harvest. MDPI 2019-11-06 /pmc/articles/PMC6918406/ /pubmed/31698744 http://dx.doi.org/10.3390/plants8110475 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhang, Yu
Zhou, Jiawu
Yang, Ying
Elgamal, Walid Hassan
Xu, Peng
Li, Jing
El-Refaee, Yasser Z.
Hao, Suding
Tao, Dayun
Two SNP Mutations Turned off Seed Shattering in Rice
title Two SNP Mutations Turned off Seed Shattering in Rice
title_full Two SNP Mutations Turned off Seed Shattering in Rice
title_fullStr Two SNP Mutations Turned off Seed Shattering in Rice
title_full_unstemmed Two SNP Mutations Turned off Seed Shattering in Rice
title_short Two SNP Mutations Turned off Seed Shattering in Rice
title_sort two snp mutations turned off seed shattering in rice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918406/
https://www.ncbi.nlm.nih.gov/pubmed/31698744
http://dx.doi.org/10.3390/plants8110475
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