Cargando…
Alcohol extract from Vernonia anthelmintica willd (L.) seed counteracts stress-induced murine hair follicle growth inhibition
BACKGROUND: Vernonia anthelmintica (L.) willd is a traditional urgur herb in China for a long history. Its alcohol extract (AVE) has been proved to promote hair follicle growth in C57BL/6 mice. We conducted this study to investigate the hair-growth effects of AVE in stressed mice and its possible me...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918677/ https://www.ncbi.nlm.nih.gov/pubmed/31847849 http://dx.doi.org/10.1186/s12906-019-2744-9 |
_version_ | 1783480640057901056 |
---|---|
author | Wang, Qian Wang, Yongxin Pang, Silin Zhou, Jia Cai, Jie Shang, Jing |
author_facet | Wang, Qian Wang, Yongxin Pang, Silin Zhou, Jia Cai, Jie Shang, Jing |
author_sort | Wang, Qian |
collection | PubMed |
description | BACKGROUND: Vernonia anthelmintica (L.) willd is a traditional urgur herb in China for a long history. Its alcohol extract (AVE) has been proved to promote hair follicle growth in C57BL/6 mice. We conducted this study to investigate the hair-growth effects of AVE in stressed mice and its possible mechanism of action. METHODS: The hair-follicle growth effects of AVE were examined by in vivo and in vitro study. We exposed C57BL/6 male mice to chronic restraint stress to induce murine hair follicle growth inhibition. The effects of AVE were examined by histological analysis, immunofluorescence for Ki67 and cytokeratin 19 immunoreactivity, western blot assay in tyrosinase and related proteins expressions and immunofluorescence for nerve fibers. In organ culture of mouse vibrissae follicles, we used substance P as a catagen-inducing factor of hair follicle growth, and measured the elongation of hair shafts and expression of neurokinin-1 receptor protein by application of AVE. RESULTS: Our results showed that AVE counteract murine hair follicle growth inhibition caused by chronic restraint stress via inducing the conversion of telogen to anagen and inhibiting catagen premature, increasing bulb keratinocytes and bulge stem cells proliferation, promoting melanogenesis, and reducing the numbers of substance P and calcitonin gene-related peptide nerve fibers. Furthermore, AVE also counteracted murine hair follicle growth inhibition caused by substance P in organ culture. CONCLUSION: These results suggest that AVE counteract stress-induced hair follicle growth inhibition in C57BL/6 mice in vivo and in vitro, and may be an effective new candidate for treatment of stress-induced hair loss. |
format | Online Article Text |
id | pubmed-6918677 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-69186772019-12-20 Alcohol extract from Vernonia anthelmintica willd (L.) seed counteracts stress-induced murine hair follicle growth inhibition Wang, Qian Wang, Yongxin Pang, Silin Zhou, Jia Cai, Jie Shang, Jing BMC Complement Altern Med Research Article BACKGROUND: Vernonia anthelmintica (L.) willd is a traditional urgur herb in China for a long history. Its alcohol extract (AVE) has been proved to promote hair follicle growth in C57BL/6 mice. We conducted this study to investigate the hair-growth effects of AVE in stressed mice and its possible mechanism of action. METHODS: The hair-follicle growth effects of AVE were examined by in vivo and in vitro study. We exposed C57BL/6 male mice to chronic restraint stress to induce murine hair follicle growth inhibition. The effects of AVE were examined by histological analysis, immunofluorescence for Ki67 and cytokeratin 19 immunoreactivity, western blot assay in tyrosinase and related proteins expressions and immunofluorescence for nerve fibers. In organ culture of mouse vibrissae follicles, we used substance P as a catagen-inducing factor of hair follicle growth, and measured the elongation of hair shafts and expression of neurokinin-1 receptor protein by application of AVE. RESULTS: Our results showed that AVE counteract murine hair follicle growth inhibition caused by chronic restraint stress via inducing the conversion of telogen to anagen and inhibiting catagen premature, increasing bulb keratinocytes and bulge stem cells proliferation, promoting melanogenesis, and reducing the numbers of substance P and calcitonin gene-related peptide nerve fibers. Furthermore, AVE also counteracted murine hair follicle growth inhibition caused by substance P in organ culture. CONCLUSION: These results suggest that AVE counteract stress-induced hair follicle growth inhibition in C57BL/6 mice in vivo and in vitro, and may be an effective new candidate for treatment of stress-induced hair loss. BioMed Central 2019-12-17 /pmc/articles/PMC6918677/ /pubmed/31847849 http://dx.doi.org/10.1186/s12906-019-2744-9 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Wang, Qian Wang, Yongxin Pang, Silin Zhou, Jia Cai, Jie Shang, Jing Alcohol extract from Vernonia anthelmintica willd (L.) seed counteracts stress-induced murine hair follicle growth inhibition |
title | Alcohol extract from Vernonia anthelmintica willd (L.) seed counteracts stress-induced murine hair follicle growth inhibition |
title_full | Alcohol extract from Vernonia anthelmintica willd (L.) seed counteracts stress-induced murine hair follicle growth inhibition |
title_fullStr | Alcohol extract from Vernonia anthelmintica willd (L.) seed counteracts stress-induced murine hair follicle growth inhibition |
title_full_unstemmed | Alcohol extract from Vernonia anthelmintica willd (L.) seed counteracts stress-induced murine hair follicle growth inhibition |
title_short | Alcohol extract from Vernonia anthelmintica willd (L.) seed counteracts stress-induced murine hair follicle growth inhibition |
title_sort | alcohol extract from vernonia anthelmintica willd (l.) seed counteracts stress-induced murine hair follicle growth inhibition |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6918677/ https://www.ncbi.nlm.nih.gov/pubmed/31847849 http://dx.doi.org/10.1186/s12906-019-2744-9 |
work_keys_str_mv | AT wangqian alcoholextractfromvernoniaanthelminticawilldlseedcounteractsstressinducedmurinehairfolliclegrowthinhibition AT wangyongxin alcoholextractfromvernoniaanthelminticawilldlseedcounteractsstressinducedmurinehairfolliclegrowthinhibition AT pangsilin alcoholextractfromvernoniaanthelminticawilldlseedcounteractsstressinducedmurinehairfolliclegrowthinhibition AT zhoujia alcoholextractfromvernoniaanthelminticawilldlseedcounteractsstressinducedmurinehairfolliclegrowthinhibition AT caijie alcoholextractfromvernoniaanthelminticawilldlseedcounteractsstressinducedmurinehairfolliclegrowthinhibition AT shangjing alcoholextractfromvernoniaanthelminticawilldlseedcounteractsstressinducedmurinehairfolliclegrowthinhibition |