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Expanding the CRISPR Toolbox with ErCas12a in Zebrafish and Human Cells

CRISPR and CRISPR-Cas effector proteins enable the targeting of DNA double-strand breaks to defined loci based on a variable length RNA guide specific to each effector. The guide RNAs are generally similar in size and form, consisting of a ∼20 nucleotide sequence complementary to the DNA target and...

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Detalles Bibliográficos
Autores principales:	Wierson, Wesley A., Simone, Brandon W., WareJoncas, Zachary, Mann, Carla, Welker, Jordan M., Kar, Bibekananda, Emch, Michael J., Friedberg, Iddo, Gendron, William A.C., Barry, Michael A., Clark, Karl J., Dobbs, Drena L., McGrail, Maura A., Ekker, Stephen C., Essner, Jeffrey J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Mary Ann Liebert, Inc., publishers 2019
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6919245/ https://www.ncbi.nlm.nih.gov/pubmed/31742435 http://dx.doi.org/10.1089/crispr.2019.0026

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