Generation of a knock-in MAP2-tdTomato reporter human embryonic stem cell line with inducible expression of NEUROG2/1 (NYGCe001-A)

Overexpression of NEUROG2 and NEUROG1 (NEUROG2/1) in human embryonic stem cells (hESCs) rapidly produces functional networks of excitatory and inhibitory neurons. To facilitate the use of this efficient inducible human neuron model in neuroscience research, we generated hESCs with doxycycline-induci...

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Detalles Bibliográficos
Autores principales: Lu, Congyi, Sanjana, Neville E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6919562/
https://www.ncbi.nlm.nih.gov/pubmed/31707212
http://dx.doi.org/10.1016/j.scr.2019.101643
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author Lu, Congyi
Sanjana, Neville E.
author_facet Lu, Congyi
Sanjana, Neville E.
author_sort Lu, Congyi
collection PubMed
description Overexpression of NEUROG2 and NEUROG1 (NEUROG2/1) in human embryonic stem cells (hESCs) rapidly produces functional networks of excitatory and inhibitory neurons. To facilitate the use of this efficient inducible human neuron model in neuroscience research, we generated hESCs with doxycycline-inducible NEUROG2/1 via lentivirus and a tdTomato fluorescent reporter knock-in at the MAP2 locus using the CRISPR nuclease Cas9. Upon doxycycline-driven induction of NEUROG2/1, these hESCs differentiate within days into cells that are uniformly MAP2 immunoreactive and tdTomato fluorescent.
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spelling pubmed-69195622019-12-18 Generation of a knock-in MAP2-tdTomato reporter human embryonic stem cell line with inducible expression of NEUROG2/1 (NYGCe001-A) Lu, Congyi Sanjana, Neville E. Stem Cell Res Article Overexpression of NEUROG2 and NEUROG1 (NEUROG2/1) in human embryonic stem cells (hESCs) rapidly produces functional networks of excitatory and inhibitory neurons. To facilitate the use of this efficient inducible human neuron model in neuroscience research, we generated hESCs with doxycycline-inducible NEUROG2/1 via lentivirus and a tdTomato fluorescent reporter knock-in at the MAP2 locus using the CRISPR nuclease Cas9. Upon doxycycline-driven induction of NEUROG2/1, these hESCs differentiate within days into cells that are uniformly MAP2 immunoreactive and tdTomato fluorescent. 2019-11-01 2019-12 /pmc/articles/PMC6919562/ /pubmed/31707212 http://dx.doi.org/10.1016/j.scr.2019.101643 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) ).
spellingShingle Article
Lu, Congyi
Sanjana, Neville E.
Generation of a knock-in MAP2-tdTomato reporter human embryonic stem cell line with inducible expression of NEUROG2/1 (NYGCe001-A)
title Generation of a knock-in MAP2-tdTomato reporter human embryonic stem cell line with inducible expression of NEUROG2/1 (NYGCe001-A)
title_full Generation of a knock-in MAP2-tdTomato reporter human embryonic stem cell line with inducible expression of NEUROG2/1 (NYGCe001-A)
title_fullStr Generation of a knock-in MAP2-tdTomato reporter human embryonic stem cell line with inducible expression of NEUROG2/1 (NYGCe001-A)
title_full_unstemmed Generation of a knock-in MAP2-tdTomato reporter human embryonic stem cell line with inducible expression of NEUROG2/1 (NYGCe001-A)
title_short Generation of a knock-in MAP2-tdTomato reporter human embryonic stem cell line with inducible expression of NEUROG2/1 (NYGCe001-A)
title_sort generation of a knock-in map2-tdtomato reporter human embryonic stem cell line with inducible expression of neurog2/1 (nygce001-a)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6919562/
https://www.ncbi.nlm.nih.gov/pubmed/31707212
http://dx.doi.org/10.1016/j.scr.2019.101643
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AT sanjananevillee generationofaknockinmap2tdtomatoreporterhumanembryonicstemcelllinewithinducibleexpressionofneurog21nygce001a

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