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Use of sodium dodecyl sulfate to improve tuberculosis sputum smear microscopy

Sputum smear microscopy (SSM), the most widely available tool for tuberculosis (TB) detection, has limited performance in paucibacillary patients and requires highly experienced technicians. The objective of this study was to determine whether the addition of sodium dodecyl sulfate (SDS), a detergen...

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Autores principales: Sarro, Yeya dit Sadio, Kodio, Ousmane, Kumar, Alisha, Diarra, Bassirou, Baya, Bocar, Diabate, Seydou, Kone, Bourahima, Sanogo, Fanta, Tolofoudie, Mohamed, Somboro, Amadou, Coulibaly, Gagni, Degoga, Boureima, Kone, Mahamadou, Dembele, Bindongo PP, Camara, Issiaka, Sanogo, Moumine, Togo, Antieme CG, Coulibaly, Nadie, Diallo, Fatimata, Dembele, Etienne, Diakite, Brehima, Doumbia, Seydou, Popoola, Oluwatoyin P, Diallo, Souleymane, Holl, Jane, Achenbach, Chad J, Murphy, Robert L., McFall, Sally, Maiga, Mamoudou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6919652/
https://www.ncbi.nlm.nih.gov/pubmed/31853521
http://dx.doi.org/10.15641/ghi.v2i2.824
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author Sarro, Yeya dit Sadio
Kodio, Ousmane
Kumar, Alisha
Diarra, Bassirou
Baya, Bocar
Diabate, Seydou
Kone, Bourahima
Sanogo, Fanta
Tolofoudie, Mohamed
Somboro, Amadou
Coulibaly, Gagni
Degoga, Boureima
Kone, Mahamadou
Dembele, Bindongo PP
Camara, Issiaka
Sanogo, Moumine
Togo, Antieme CG
Coulibaly, Nadie
Diallo, Fatimata
Dembele, Etienne
Diakite, Brehima
Doumbia, Seydou
Popoola, Oluwatoyin P
Diallo, Souleymane
Holl, Jane
Achenbach, Chad J
Murphy, Robert L.
McFall, Sally
Maiga, Mamoudou
author_facet Sarro, Yeya dit Sadio
Kodio, Ousmane
Kumar, Alisha
Diarra, Bassirou
Baya, Bocar
Diabate, Seydou
Kone, Bourahima
Sanogo, Fanta
Tolofoudie, Mohamed
Somboro, Amadou
Coulibaly, Gagni
Degoga, Boureima
Kone, Mahamadou
Dembele, Bindongo PP
Camara, Issiaka
Sanogo, Moumine
Togo, Antieme CG
Coulibaly, Nadie
Diallo, Fatimata
Dembele, Etienne
Diakite, Brehima
Doumbia, Seydou
Popoola, Oluwatoyin P
Diallo, Souleymane
Holl, Jane
Achenbach, Chad J
Murphy, Robert L.
McFall, Sally
Maiga, Mamoudou
author_sort Sarro, Yeya dit Sadio
collection PubMed
description Sputum smear microscopy (SSM), the most widely available tool for tuberculosis (TB) detection, has limited performance in paucibacillary patients and requires highly experienced technicians. The objective of this study was to determine whether the addition of sodium dodecyl sulfate (SDS), a detergent that thins sputum, at 4% and 10%, improves the detection of acid-fast bacilli (AFB), the clarity of slides, and the biosafety of the technique. Thirty participants with presumptive TB were enrolled. Three independent, blinded technicians examined the slides. Regular sputum concentrated AFB smear and sputum culture were used as standard control methods. Sputum culture was also performed before and after 10% SDS addition for safety analysis. We found that neither SSM with SDS 4% nor SSM with SDS 10% improved the test’s performance. However, slides with 4% and 10% SDS, compared with slides prepared without SDS, had significantly better clarity scores. The 10% SDS-prepared sputum samples were all culture negative. While adding SDS detergent does not improve the performance of SSM slides, it does improve the clarity and biosafety. Where experienced technicians are scarce, especially in low resource settings, use of SDS may enhance the ease of slide reading in sputum smear microscopy.
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spelling pubmed-69196522019-12-18 Use of sodium dodecyl sulfate to improve tuberculosis sputum smear microscopy Sarro, Yeya dit Sadio Kodio, Ousmane Kumar, Alisha Diarra, Bassirou Baya, Bocar Diabate, Seydou Kone, Bourahima Sanogo, Fanta Tolofoudie, Mohamed Somboro, Amadou Coulibaly, Gagni Degoga, Boureima Kone, Mahamadou Dembele, Bindongo PP Camara, Issiaka Sanogo, Moumine Togo, Antieme CG Coulibaly, Nadie Diallo, Fatimata Dembele, Etienne Diakite, Brehima Doumbia, Seydou Popoola, Oluwatoyin P Diallo, Souleymane Holl, Jane Achenbach, Chad J Murphy, Robert L. McFall, Sally Maiga, Mamoudou Glob Health Innov Article Sputum smear microscopy (SSM), the most widely available tool for tuberculosis (TB) detection, has limited performance in paucibacillary patients and requires highly experienced technicians. The objective of this study was to determine whether the addition of sodium dodecyl sulfate (SDS), a detergent that thins sputum, at 4% and 10%, improves the detection of acid-fast bacilli (AFB), the clarity of slides, and the biosafety of the technique. Thirty participants with presumptive TB were enrolled. Three independent, blinded technicians examined the slides. Regular sputum concentrated AFB smear and sputum culture were used as standard control methods. Sputum culture was also performed before and after 10% SDS addition for safety analysis. We found that neither SSM with SDS 4% nor SSM with SDS 10% improved the test’s performance. However, slides with 4% and 10% SDS, compared with slides prepared without SDS, had significantly better clarity scores. The 10% SDS-prepared sputum samples were all culture negative. While adding SDS detergent does not improve the performance of SSM slides, it does improve the clarity and biosafety. Where experienced technicians are scarce, especially in low resource settings, use of SDS may enhance the ease of slide reading in sputum smear microscopy. 2019-11-26 2019-11-27 /pmc/articles/PMC6919652/ /pubmed/31853521 http://dx.doi.org/10.15641/ghi.v2i2.824 Text en http://creativecommons.org/licenses/by/4.0/ Published under a Creative Commons CC-BY License.
spellingShingle Article
Sarro, Yeya dit Sadio
Kodio, Ousmane
Kumar, Alisha
Diarra, Bassirou
Baya, Bocar
Diabate, Seydou
Kone, Bourahima
Sanogo, Fanta
Tolofoudie, Mohamed
Somboro, Amadou
Coulibaly, Gagni
Degoga, Boureima
Kone, Mahamadou
Dembele, Bindongo PP
Camara, Issiaka
Sanogo, Moumine
Togo, Antieme CG
Coulibaly, Nadie
Diallo, Fatimata
Dembele, Etienne
Diakite, Brehima
Doumbia, Seydou
Popoola, Oluwatoyin P
Diallo, Souleymane
Holl, Jane
Achenbach, Chad J
Murphy, Robert L.
McFall, Sally
Maiga, Mamoudou
Use of sodium dodecyl sulfate to improve tuberculosis sputum smear microscopy
title Use of sodium dodecyl sulfate to improve tuberculosis sputum smear microscopy
title_full Use of sodium dodecyl sulfate to improve tuberculosis sputum smear microscopy
title_fullStr Use of sodium dodecyl sulfate to improve tuberculosis sputum smear microscopy
title_full_unstemmed Use of sodium dodecyl sulfate to improve tuberculosis sputum smear microscopy
title_short Use of sodium dodecyl sulfate to improve tuberculosis sputum smear microscopy
title_sort use of sodium dodecyl sulfate to improve tuberculosis sputum smear microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6919652/
https://www.ncbi.nlm.nih.gov/pubmed/31853521
http://dx.doi.org/10.15641/ghi.v2i2.824
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