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Dunaliella salina microalgae oppose thioacetamide-induced hepatic fibrosis in rats
Several hepatic pathological conditions are correlated with the stimulation of hepatic stellate cells. This induces a cascade of events producing accretion of extracellular matrix components triggering fibrosis. Dunaliella salina, rich in carotenoids, was investigated for its potential antagonizing...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920116/ https://www.ncbi.nlm.nih.gov/pubmed/31879596 http://dx.doi.org/10.1016/j.toxrep.2019.10.017 |
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author | El-Baz, Farouk K. Salama, Abeer A.A. Hussein, Rehab A. |
author_facet | El-Baz, Farouk K. Salama, Abeer A.A. Hussein, Rehab A. |
author_sort | El-Baz, Farouk K. |
collection | PubMed |
description | Several hepatic pathological conditions are correlated with the stimulation of hepatic stellate cells. This induces a cascade of events producing accretion of extracellular matrix components triggering fibrosis. Dunaliella salina, rich in carotenoids, was investigated for its potential antagonizing activity; functionally and structurally against thioacetamide (TAA) - induced hepatic fibrosis in rats. Adult male albino Wistar rats were treated with three dose levels of D. salina powder or extract (daily, p.o.); for 6 weeks, concomitantly with TAA injection. Serum levels of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), bilirubin and albumin were determined. Reduced glutathione (GSH), malondialdehyde (MDA), smooth muscle actin alpha (α-SMA) and collagen I hepatic contents were also estimated. Treatment with D. salina powder or extract caused a significant decline in serum levels of AST, ALT, ALP, bilirubin, MDA and hepatic contents of α-SMA and collagen I. Additionally, serum albumin and GSH hepatic content were highly elevated. Liver histopathological examination also indicated that D. salina reduced fibrosis, centrilobular necrosis, and inflammatory cell infiltration evoked by TAA. The results implied that D. salina exerts protective action against TAA-induced hepatic fibrosis in rats. The phytochemical investigation revealed high total carotenoid content prominently β-carotene (15.2 % of the algal extract) as well as unsaturated fatty acids as alpha-linolenic acid which accounts for the hepatoprotective activity. |
format | Online Article Text |
id | pubmed-6920116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-69201162019-12-26 Dunaliella salina microalgae oppose thioacetamide-induced hepatic fibrosis in rats El-Baz, Farouk K. Salama, Abeer A.A. Hussein, Rehab A. Toxicol Rep Regular Article Several hepatic pathological conditions are correlated with the stimulation of hepatic stellate cells. This induces a cascade of events producing accretion of extracellular matrix components triggering fibrosis. Dunaliella salina, rich in carotenoids, was investigated for its potential antagonizing activity; functionally and structurally against thioacetamide (TAA) - induced hepatic fibrosis in rats. Adult male albino Wistar rats were treated with three dose levels of D. salina powder or extract (daily, p.o.); for 6 weeks, concomitantly with TAA injection. Serum levels of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), bilirubin and albumin were determined. Reduced glutathione (GSH), malondialdehyde (MDA), smooth muscle actin alpha (α-SMA) and collagen I hepatic contents were also estimated. Treatment with D. salina powder or extract caused a significant decline in serum levels of AST, ALT, ALP, bilirubin, MDA and hepatic contents of α-SMA and collagen I. Additionally, serum albumin and GSH hepatic content were highly elevated. Liver histopathological examination also indicated that D. salina reduced fibrosis, centrilobular necrosis, and inflammatory cell infiltration evoked by TAA. The results implied that D. salina exerts protective action against TAA-induced hepatic fibrosis in rats. The phytochemical investigation revealed high total carotenoid content prominently β-carotene (15.2 % of the algal extract) as well as unsaturated fatty acids as alpha-linolenic acid which accounts for the hepatoprotective activity. Elsevier 2019-11-10 /pmc/articles/PMC6920116/ /pubmed/31879596 http://dx.doi.org/10.1016/j.toxrep.2019.10.017 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Regular Article El-Baz, Farouk K. Salama, Abeer A.A. Hussein, Rehab A. Dunaliella salina microalgae oppose thioacetamide-induced hepatic fibrosis in rats |
title | Dunaliella salina microalgae oppose thioacetamide-induced hepatic fibrosis in rats |
title_full | Dunaliella salina microalgae oppose thioacetamide-induced hepatic fibrosis in rats |
title_fullStr | Dunaliella salina microalgae oppose thioacetamide-induced hepatic fibrosis in rats |
title_full_unstemmed | Dunaliella salina microalgae oppose thioacetamide-induced hepatic fibrosis in rats |
title_short | Dunaliella salina microalgae oppose thioacetamide-induced hepatic fibrosis in rats |
title_sort | dunaliella salina microalgae oppose thioacetamide-induced hepatic fibrosis in rats |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920116/ https://www.ncbi.nlm.nih.gov/pubmed/31879596 http://dx.doi.org/10.1016/j.toxrep.2019.10.017 |
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