High-Temperature Liquid Chromatography and the Hyphenation with Mass Spectrometry Using High-Pressure Electrospray Ionization

Increasing the operating temperature of the liquid chromatography (LC) column has the same effect as reducing the diameter of the packing particles on minimizing the contribution of C-term in the van Deemter equation, flattening the curve of plate height vs. linear velocity in the high-speed region, thus allowing a fast LC analysis without the loss of plate count. While the use of smaller particles requires a higher pumping pressure, operating the column at higher temperature reduces the pressure due to lower liquid viscosity. At present, the adoption of high-temperature LC lags behind the ultra-high-pressure LC. Nevertheless, the availability of thermally stable columns has steadily improved and new innovations in this area have continued to emerge. This paper gives a brief review and updates on the recent advances in high-temperature liquid chromatography (HTLC). Recent efforts of hyphenating the capillary HTLC with mass spectrometry via a super-atmospheric pressure electrospray ionization is also reported.