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Ultrastructural modeling of small angle scattering from photosynthetic membranes
The last decade has seen a range of studies using non-invasive neutron and X-ray techniques to probe the ultrastructure of a variety of photosynthetic membrane systems. A common denominator in this work is the lack of an explicitly formulated underlying structural model, ultimately leading to ambigu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920412/ https://www.ncbi.nlm.nih.gov/pubmed/31852917 http://dx.doi.org/10.1038/s41598-019-55423-0 |
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author | Jakubauskas, Dainius Kowalewska, Łucja Sokolova, Anna V. Garvey, Christopher J. Mortensen, Kell Jensen, Poul Erik Kirkensgaard, Jacob J. K. |
author_facet | Jakubauskas, Dainius Kowalewska, Łucja Sokolova, Anna V. Garvey, Christopher J. Mortensen, Kell Jensen, Poul Erik Kirkensgaard, Jacob J. K. |
author_sort | Jakubauskas, Dainius |
collection | PubMed |
description | The last decade has seen a range of studies using non-invasive neutron and X-ray techniques to probe the ultrastructure of a variety of photosynthetic membrane systems. A common denominator in this work is the lack of an explicitly formulated underlying structural model, ultimately leading to ambiguity in the data interpretation. Here we formulate and implement a full mathematical model of the scattering from a stacked double bilayer membrane system taking instrumental resolution and polydispersity into account. We validate our model by direct simulation of scattering patterns from 3D structural models. Most importantly, we demonstrate that the full scattering curves from three structurally typical cyanobacterial thylakoid membrane systems measured in vivo can all be described within this framework. The model provides realistic estimates of key structural parameters in the thylakoid membrane, in particular the overall stacking distance and how this is divided between membranes, lumen and cytoplasmic liquid. Finally, from fitted scattering length densities it becomes clear that the protein content in the inner lumen has to be lower than in the outer cytoplasmic liquid and we extract the first quantitative measure of the luminal protein content in a living cyanobacteria. |
format | Online Article Text |
id | pubmed-6920412 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69204122019-12-20 Ultrastructural modeling of small angle scattering from photosynthetic membranes Jakubauskas, Dainius Kowalewska, Łucja Sokolova, Anna V. Garvey, Christopher J. Mortensen, Kell Jensen, Poul Erik Kirkensgaard, Jacob J. K. Sci Rep Article The last decade has seen a range of studies using non-invasive neutron and X-ray techniques to probe the ultrastructure of a variety of photosynthetic membrane systems. A common denominator in this work is the lack of an explicitly formulated underlying structural model, ultimately leading to ambiguity in the data interpretation. Here we formulate and implement a full mathematical model of the scattering from a stacked double bilayer membrane system taking instrumental resolution and polydispersity into account. We validate our model by direct simulation of scattering patterns from 3D structural models. Most importantly, we demonstrate that the full scattering curves from three structurally typical cyanobacterial thylakoid membrane systems measured in vivo can all be described within this framework. The model provides realistic estimates of key structural parameters in the thylakoid membrane, in particular the overall stacking distance and how this is divided between membranes, lumen and cytoplasmic liquid. Finally, from fitted scattering length densities it becomes clear that the protein content in the inner lumen has to be lower than in the outer cytoplasmic liquid and we extract the first quantitative measure of the luminal protein content in a living cyanobacteria. Nature Publishing Group UK 2019-12-18 /pmc/articles/PMC6920412/ /pubmed/31852917 http://dx.doi.org/10.1038/s41598-019-55423-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Jakubauskas, Dainius Kowalewska, Łucja Sokolova, Anna V. Garvey, Christopher J. Mortensen, Kell Jensen, Poul Erik Kirkensgaard, Jacob J. K. Ultrastructural modeling of small angle scattering from photosynthetic membranes |
title | Ultrastructural modeling of small angle scattering from photosynthetic membranes |
title_full | Ultrastructural modeling of small angle scattering from photosynthetic membranes |
title_fullStr | Ultrastructural modeling of small angle scattering from photosynthetic membranes |
title_full_unstemmed | Ultrastructural modeling of small angle scattering from photosynthetic membranes |
title_short | Ultrastructural modeling of small angle scattering from photosynthetic membranes |
title_sort | ultrastructural modeling of small angle scattering from photosynthetic membranes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920412/ https://www.ncbi.nlm.nih.gov/pubmed/31852917 http://dx.doi.org/10.1038/s41598-019-55423-0 |
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