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Influence of Solvent Composition and Surface Tension on the Signal Intensity of Amino Acids in Electrospray Ionization Mass Spectrometry
The influence of solvent composition and surface tension on the signal intensity of deprotonated molecules [M−H](−) in electrospray ionization mass spectrometry (ESI MS) was evaluated using alanine (Ala), threonine (Thr) and phenylalanine (Phe), which have differing levels of hydrophobicity. The sur...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Mass Spectrometry Society of Japan
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920628/ https://www.ncbi.nlm.nih.gov/pubmed/32010543 http://dx.doi.org/10.5702/massspectrometry.A0077 |
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author | Kageyama (Kaneshima), Ami Motoyama, Akira Takayama, Mitsuo |
author_facet | Kageyama (Kaneshima), Ami Motoyama, Akira Takayama, Mitsuo |
author_sort | Kageyama (Kaneshima), Ami |
collection | PubMed |
description | The influence of solvent composition and surface tension on the signal intensity of deprotonated molecules [M−H](−) in electrospray ionization mass spectrometry (ESI MS) was evaluated using alanine (Ala), threonine (Thr) and phenylalanine (Phe), which have differing levels of hydrophobicity. The surface tension of the ESI solution was varied by changing the ratio of the organic solvents methanol (MeOH) and acetonitrile (MeCN) in water (H(2)O). In ESI MS, the signal intensity of all the amino acids was increased with decreasing surface tension for the two solutions, H(2)O/MeOH and H(2)O/MeCN. The use of H(2)O/MeCN was more favorable for achieving a strong signal for the analytes compared to H(2)O/MeOH. The smaller vaporization enthalpy of MeCN compared to MeOH was proposed as one of the most plausible explanation for this. The order of the signal intensity of amino acids was Phe>Thr>Ala, the same order as their hydrophobicity. It can be practically concluded that the use of solutions with lower surface tensions and lower vaporization enthalpies would result in higher signal intensities in ESI MS. |
format | Online Article Text |
id | pubmed-6920628 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Mass Spectrometry Society of Japan |
record_format | MEDLINE/PubMed |
spelling | pubmed-69206282020-01-31 Influence of Solvent Composition and Surface Tension on the Signal Intensity of Amino Acids in Electrospray Ionization Mass Spectrometry Kageyama (Kaneshima), Ami Motoyama, Akira Takayama, Mitsuo Mass Spectrom (Tokyo) Original Article The influence of solvent composition and surface tension on the signal intensity of deprotonated molecules [M−H](−) in electrospray ionization mass spectrometry (ESI MS) was evaluated using alanine (Ala), threonine (Thr) and phenylalanine (Phe), which have differing levels of hydrophobicity. The surface tension of the ESI solution was varied by changing the ratio of the organic solvents methanol (MeOH) and acetonitrile (MeCN) in water (H(2)O). In ESI MS, the signal intensity of all the amino acids was increased with decreasing surface tension for the two solutions, H(2)O/MeOH and H(2)O/MeCN. The use of H(2)O/MeCN was more favorable for achieving a strong signal for the analytes compared to H(2)O/MeOH. The smaller vaporization enthalpy of MeCN compared to MeOH was proposed as one of the most plausible explanation for this. The order of the signal intensity of amino acids was Phe>Thr>Ala, the same order as their hydrophobicity. It can be practically concluded that the use of solutions with lower surface tensions and lower vaporization enthalpies would result in higher signal intensities in ESI MS. The Mass Spectrometry Society of Japan 2019 2019-11-30 /pmc/articles/PMC6920628/ /pubmed/32010543 http://dx.doi.org/10.5702/massspectrometry.A0077 Text en Copyright © 2019 Ami Kageyama (Kaneshima), Akira Motoyama, and Mitsuo Takayama. http://creativecommons.org/licenses/by/2.5/ This is an open access article distributed under the terms of Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Original Article Kageyama (Kaneshima), Ami Motoyama, Akira Takayama, Mitsuo Influence of Solvent Composition and Surface Tension on the Signal Intensity of Amino Acids in Electrospray Ionization Mass Spectrometry |
title | Influence of Solvent Composition and Surface Tension on the Signal Intensity of Amino Acids in Electrospray Ionization Mass Spectrometry |
title_full | Influence of Solvent Composition and Surface Tension on the Signal Intensity of Amino Acids in Electrospray Ionization Mass Spectrometry |
title_fullStr | Influence of Solvent Composition and Surface Tension on the Signal Intensity of Amino Acids in Electrospray Ionization Mass Spectrometry |
title_full_unstemmed | Influence of Solvent Composition and Surface Tension on the Signal Intensity of Amino Acids in Electrospray Ionization Mass Spectrometry |
title_short | Influence of Solvent Composition and Surface Tension on the Signal Intensity of Amino Acids in Electrospray Ionization Mass Spectrometry |
title_sort | influence of solvent composition and surface tension on the signal intensity of amino acids in electrospray ionization mass spectrometry |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920628/ https://www.ncbi.nlm.nih.gov/pubmed/32010543 http://dx.doi.org/10.5702/massspectrometry.A0077 |
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