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Non-Invasive Approach for Evaluation of Pulmonary Hypertension Using Extracellular Vesicle-Associated Small Non-Coding RNA

Extracellular vesicles are released by numerous cell types of the human body under physiological but also under pathophysiological conditions. They are important for cell–cell communication and carry specific signatures of peptides and RNAs. In this study, we aimed to determine whether extracellular...

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Autores principales: Lipps, Christoph, Northe, Philipp, Figueiredo, Ricardo, Rohde, Manfred, Brahmer, Alexandra, Krämer-Albers, Eva-Maria, Liebetrau, Christoph, Wiedenroth, Christoph B., Mayer, Eckhard, Kriechbaum, Steffen D., Dörr, Oliver, Nef, Holger, Hamm, Christian W., Keller, Till, Troidl, Christian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920761/
https://www.ncbi.nlm.nih.gov/pubmed/31671920
http://dx.doi.org/10.3390/biom9110666
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author Lipps, Christoph
Northe, Philipp
Figueiredo, Ricardo
Rohde, Manfred
Brahmer, Alexandra
Krämer-Albers, Eva-Maria
Liebetrau, Christoph
Wiedenroth, Christoph B.
Mayer, Eckhard
Kriechbaum, Steffen D.
Dörr, Oliver
Nef, Holger
Hamm, Christian W.
Keller, Till
Troidl, Christian
author_facet Lipps, Christoph
Northe, Philipp
Figueiredo, Ricardo
Rohde, Manfred
Brahmer, Alexandra
Krämer-Albers, Eva-Maria
Liebetrau, Christoph
Wiedenroth, Christoph B.
Mayer, Eckhard
Kriechbaum, Steffen D.
Dörr, Oliver
Nef, Holger
Hamm, Christian W.
Keller, Till
Troidl, Christian
author_sort Lipps, Christoph
collection PubMed
description Extracellular vesicles are released by numerous cell types of the human body under physiological but also under pathophysiological conditions. They are important for cell–cell communication and carry specific signatures of peptides and RNAs. In this study, we aimed to determine whether extracellular vesicles isolated from patients with pulmonary hypertension show a disease specific signature of small non-coding RNAs and thus have the potential to serve as diagnostic and prognostic biomarkers. Extracellular vesicles were isolated from the serum of 23 patients with chronic thromboembolic pulmonary hypertension (CTEPH) and 23 controls using two individual methods: a column-based method or by precipitation. Extracellular vesicle- associated RNAs were analyzed by next-generation sequencing applying molecular barcoding, and differentially expressed small non-coding RNAs were validated by quantitative real-time polymerase chain reaction (qRT-PCR). We identified 18 microRNAs and 21 P-element induced wimpy testis (PIWI)-interacting RNAs (piRNAs) or piRNA clusters that were differentially expressed in CTEPH patients compared with controls. Bioinformatic analysis predicted a contribution of these piRNAs to the progression of cardiac and vascular remodeling. Expression levels of DQ593039 correlated with clinically meaningful parameters such as mean pulmonary arterial pressure, pulmonary vascular resistance, right ventricular systolic pressure, and levels of N-terminal pro-brain natriuretic peptide. Thus, we identified the extracellular vesicle- derived piRNA, DQ593039, as a potential biomarker for pulmonary hypertension and right heart disease.
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spelling pubmed-69207612019-12-24 Non-Invasive Approach for Evaluation of Pulmonary Hypertension Using Extracellular Vesicle-Associated Small Non-Coding RNA Lipps, Christoph Northe, Philipp Figueiredo, Ricardo Rohde, Manfred Brahmer, Alexandra Krämer-Albers, Eva-Maria Liebetrau, Christoph Wiedenroth, Christoph B. Mayer, Eckhard Kriechbaum, Steffen D. Dörr, Oliver Nef, Holger Hamm, Christian W. Keller, Till Troidl, Christian Biomolecules Article Extracellular vesicles are released by numerous cell types of the human body under physiological but also under pathophysiological conditions. They are important for cell–cell communication and carry specific signatures of peptides and RNAs. In this study, we aimed to determine whether extracellular vesicles isolated from patients with pulmonary hypertension show a disease specific signature of small non-coding RNAs and thus have the potential to serve as diagnostic and prognostic biomarkers. Extracellular vesicles were isolated from the serum of 23 patients with chronic thromboembolic pulmonary hypertension (CTEPH) and 23 controls using two individual methods: a column-based method or by precipitation. Extracellular vesicle- associated RNAs were analyzed by next-generation sequencing applying molecular barcoding, and differentially expressed small non-coding RNAs were validated by quantitative real-time polymerase chain reaction (qRT-PCR). We identified 18 microRNAs and 21 P-element induced wimpy testis (PIWI)-interacting RNAs (piRNAs) or piRNA clusters that were differentially expressed in CTEPH patients compared with controls. Bioinformatic analysis predicted a contribution of these piRNAs to the progression of cardiac and vascular remodeling. Expression levels of DQ593039 correlated with clinically meaningful parameters such as mean pulmonary arterial pressure, pulmonary vascular resistance, right ventricular systolic pressure, and levels of N-terminal pro-brain natriuretic peptide. Thus, we identified the extracellular vesicle- derived piRNA, DQ593039, as a potential biomarker for pulmonary hypertension and right heart disease. MDPI 2019-10-29 /pmc/articles/PMC6920761/ /pubmed/31671920 http://dx.doi.org/10.3390/biom9110666 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lipps, Christoph
Northe, Philipp
Figueiredo, Ricardo
Rohde, Manfred
Brahmer, Alexandra
Krämer-Albers, Eva-Maria
Liebetrau, Christoph
Wiedenroth, Christoph B.
Mayer, Eckhard
Kriechbaum, Steffen D.
Dörr, Oliver
Nef, Holger
Hamm, Christian W.
Keller, Till
Troidl, Christian
Non-Invasive Approach for Evaluation of Pulmonary Hypertension Using Extracellular Vesicle-Associated Small Non-Coding RNA
title Non-Invasive Approach for Evaluation of Pulmonary Hypertension Using Extracellular Vesicle-Associated Small Non-Coding RNA
title_full Non-Invasive Approach for Evaluation of Pulmonary Hypertension Using Extracellular Vesicle-Associated Small Non-Coding RNA
title_fullStr Non-Invasive Approach for Evaluation of Pulmonary Hypertension Using Extracellular Vesicle-Associated Small Non-Coding RNA
title_full_unstemmed Non-Invasive Approach for Evaluation of Pulmonary Hypertension Using Extracellular Vesicle-Associated Small Non-Coding RNA
title_short Non-Invasive Approach for Evaluation of Pulmonary Hypertension Using Extracellular Vesicle-Associated Small Non-Coding RNA
title_sort non-invasive approach for evaluation of pulmonary hypertension using extracellular vesicle-associated small non-coding rna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920761/
https://www.ncbi.nlm.nih.gov/pubmed/31671920
http://dx.doi.org/10.3390/biom9110666
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