Cargando…
Fermented Oyster Extract Promotes Osteoblast Differentiation by Activating the Wnt/β-Catenin Signaling Pathway, Leading to Bone Formation
The Pacific oyster, Crassostrea gigas, is well-known as a nutritious food. Recently, we revealed that fermented extract of C. gigas (FO) inhibited ovariectomy-induced osteoporosis, resulting from suppression of osteoclastogenesis. However, since the beneficial effect of FO on osteogenesis is poorly...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920898/ https://www.ncbi.nlm.nih.gov/pubmed/31698882 http://dx.doi.org/10.3390/biom9110711 |
_version_ | 1783481038723350528 |
---|---|
author | Molagoda, Ilandarage Menu Neelaka Karunarathne, Wisurumuni Arachchilage Hasitha Maduranga Choi, Yung Hyun Park, Eui Kyun Jeon, You-Jin Lee, Bae-Jin Kang, Chang-Hee Kim, Gi-Young |
author_facet | Molagoda, Ilandarage Menu Neelaka Karunarathne, Wisurumuni Arachchilage Hasitha Maduranga Choi, Yung Hyun Park, Eui Kyun Jeon, You-Jin Lee, Bae-Jin Kang, Chang-Hee Kim, Gi-Young |
author_sort | Molagoda, Ilandarage Menu Neelaka |
collection | PubMed |
description | The Pacific oyster, Crassostrea gigas, is well-known as a nutritious food. Recently, we revealed that fermented extract of C. gigas (FO) inhibited ovariectomy-induced osteoporosis, resulting from suppression of osteoclastogenesis. However, since the beneficial effect of FO on osteogenesis is poorly understood, it was examined in mouse preosteoblast MC3T3-E1 cells, human osteosarcoma MG-63 osteoblast-like cells, and zebrafish larvae in this study. We found that FO increased mitochondrial activity from days 1 to 7; however, total cell number of MC3T3-E1 cells gradually decreased without any change in cell viability, which suggests that FO stimulates the differentiation of MC3T3-E1 cells. FO also promoted the expression of osteoblast marker genes, including runt-related transcription factor 2 (mRUNX2), alkaline phosphatase (mALP), collagen type I α1 (mCol1α1), osteocalcin (mOCN), osterix (mOSX), bone morphogenetic protein 2 (mBMP2), and mBMP4 in MC3T3-E1 cells accompanied by a significant increase in ALP activity. FO also increased nuclear translocation of RUNX2 and OSX transcription factors, ALP activity, and calcification in vitro along with the upregulated expression of osteoblast-specific marker proteins such as RUNX2, ALP, Col1α1, OCN, OSX, and BMP4. Additionally, FO enhanced bone mineralization (calcein intensity) in zebrafish larvae at 9 days post-fertilization comparable to that in the β-glycerophosphate (GP)-treated group. All the tested osteoblast marker genes, including zRUNX2a, zRUNX2b, zALP, zCol1a1, zOCN, zBMP2, and zBMP4, were also remarkably upregulated in the zebrafish larvae in response to FO. It also promoted tail fin regeneration in adult zebrafish as same as the GP-treated groups. Furthermore, not only FO positively regulate β-catenin expression and Wnt/β-catenin luciferase activity, but pretreatment with a Wnt/β-catenin inhibitor (FH535) also significantly decreased FO-mediated bone mineralization in zebrafish larvae, which indicates that FO-induced osteogenesis depends on the Wnt/β-catenin pathway. Altogether, the current study suggests that the supplemental intake of FO has a beneficial effect on osteogenesis. |
format | Online Article Text |
id | pubmed-6920898 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69208982019-12-24 Fermented Oyster Extract Promotes Osteoblast Differentiation by Activating the Wnt/β-Catenin Signaling Pathway, Leading to Bone Formation Molagoda, Ilandarage Menu Neelaka Karunarathne, Wisurumuni Arachchilage Hasitha Maduranga Choi, Yung Hyun Park, Eui Kyun Jeon, You-Jin Lee, Bae-Jin Kang, Chang-Hee Kim, Gi-Young Biomolecules Article The Pacific oyster, Crassostrea gigas, is well-known as a nutritious food. Recently, we revealed that fermented extract of C. gigas (FO) inhibited ovariectomy-induced osteoporosis, resulting from suppression of osteoclastogenesis. However, since the beneficial effect of FO on osteogenesis is poorly understood, it was examined in mouse preosteoblast MC3T3-E1 cells, human osteosarcoma MG-63 osteoblast-like cells, and zebrafish larvae in this study. We found that FO increased mitochondrial activity from days 1 to 7; however, total cell number of MC3T3-E1 cells gradually decreased without any change in cell viability, which suggests that FO stimulates the differentiation of MC3T3-E1 cells. FO also promoted the expression of osteoblast marker genes, including runt-related transcription factor 2 (mRUNX2), alkaline phosphatase (mALP), collagen type I α1 (mCol1α1), osteocalcin (mOCN), osterix (mOSX), bone morphogenetic protein 2 (mBMP2), and mBMP4 in MC3T3-E1 cells accompanied by a significant increase in ALP activity. FO also increased nuclear translocation of RUNX2 and OSX transcription factors, ALP activity, and calcification in vitro along with the upregulated expression of osteoblast-specific marker proteins such as RUNX2, ALP, Col1α1, OCN, OSX, and BMP4. Additionally, FO enhanced bone mineralization (calcein intensity) in zebrafish larvae at 9 days post-fertilization comparable to that in the β-glycerophosphate (GP)-treated group. All the tested osteoblast marker genes, including zRUNX2a, zRUNX2b, zALP, zCol1a1, zOCN, zBMP2, and zBMP4, were also remarkably upregulated in the zebrafish larvae in response to FO. It also promoted tail fin regeneration in adult zebrafish as same as the GP-treated groups. Furthermore, not only FO positively regulate β-catenin expression and Wnt/β-catenin luciferase activity, but pretreatment with a Wnt/β-catenin inhibitor (FH535) also significantly decreased FO-mediated bone mineralization in zebrafish larvae, which indicates that FO-induced osteogenesis depends on the Wnt/β-catenin pathway. Altogether, the current study suggests that the supplemental intake of FO has a beneficial effect on osteogenesis. MDPI 2019-11-06 /pmc/articles/PMC6920898/ /pubmed/31698882 http://dx.doi.org/10.3390/biom9110711 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Molagoda, Ilandarage Menu Neelaka Karunarathne, Wisurumuni Arachchilage Hasitha Maduranga Choi, Yung Hyun Park, Eui Kyun Jeon, You-Jin Lee, Bae-Jin Kang, Chang-Hee Kim, Gi-Young Fermented Oyster Extract Promotes Osteoblast Differentiation by Activating the Wnt/β-Catenin Signaling Pathway, Leading to Bone Formation |
title | Fermented Oyster Extract Promotes Osteoblast Differentiation by Activating the Wnt/β-Catenin Signaling Pathway, Leading to Bone Formation |
title_full | Fermented Oyster Extract Promotes Osteoblast Differentiation by Activating the Wnt/β-Catenin Signaling Pathway, Leading to Bone Formation |
title_fullStr | Fermented Oyster Extract Promotes Osteoblast Differentiation by Activating the Wnt/β-Catenin Signaling Pathway, Leading to Bone Formation |
title_full_unstemmed | Fermented Oyster Extract Promotes Osteoblast Differentiation by Activating the Wnt/β-Catenin Signaling Pathway, Leading to Bone Formation |
title_short | Fermented Oyster Extract Promotes Osteoblast Differentiation by Activating the Wnt/β-Catenin Signaling Pathway, Leading to Bone Formation |
title_sort | fermented oyster extract promotes osteoblast differentiation by activating the wnt/β-catenin signaling pathway, leading to bone formation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6920898/ https://www.ncbi.nlm.nih.gov/pubmed/31698882 http://dx.doi.org/10.3390/biom9110711 |
work_keys_str_mv | AT molagodailandaragemenuneelaka fermentedoysterextractpromotesosteoblastdifferentiationbyactivatingthewntbcateninsignalingpathwayleadingtoboneformation AT karunarathnewisurumuniarachchilagehasithamaduranga fermentedoysterextractpromotesosteoblastdifferentiationbyactivatingthewntbcateninsignalingpathwayleadingtoboneformation AT choiyunghyun fermentedoysterextractpromotesosteoblastdifferentiationbyactivatingthewntbcateninsignalingpathwayleadingtoboneformation AT parkeuikyun fermentedoysterextractpromotesosteoblastdifferentiationbyactivatingthewntbcateninsignalingpathwayleadingtoboneformation AT jeonyoujin fermentedoysterextractpromotesosteoblastdifferentiationbyactivatingthewntbcateninsignalingpathwayleadingtoboneformation AT leebaejin fermentedoysterextractpromotesosteoblastdifferentiationbyactivatingthewntbcateninsignalingpathwayleadingtoboneformation AT kangchanghee fermentedoysterextractpromotesosteoblastdifferentiationbyactivatingthewntbcateninsignalingpathwayleadingtoboneformation AT kimgiyoung fermentedoysterextractpromotesosteoblastdifferentiationbyactivatingthewntbcateninsignalingpathwayleadingtoboneformation |