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Isolation and Characterization of a Novel Pathogenic Strain of Ehrlichia minasensis

The genus Ehrlichia is composed of tick-borne obligate intracellular gram-negative alphaproteobacteria of the family Anaplasmataceae. Ehrlichia includes important pathogens affecting canids (E. canis, E. chaffeensis, and E. ewingii), rodents (E. muris), and ruminants (E. ruminantium). Ehrlichia mina...

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Autores principales: Moura de Aguiar, Daniel, Pessoa Araújo Junior, João, Nakazato, Luciano, Bard, Emilie, Aguilar-Bultet, Lisandra, Vorimore, Fabien, Leonidovich Popov, Vsevolod, Moleta Colodel, Edson, Cabezas-Cruz, Alejandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921006/
https://www.ncbi.nlm.nih.gov/pubmed/31694172
http://dx.doi.org/10.3390/microorganisms7110528
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author Moura de Aguiar, Daniel
Pessoa Araújo Junior, João
Nakazato, Luciano
Bard, Emilie
Aguilar-Bultet, Lisandra
Vorimore, Fabien
Leonidovich Popov, Vsevolod
Moleta Colodel, Edson
Cabezas-Cruz, Alejandro
author_facet Moura de Aguiar, Daniel
Pessoa Araújo Junior, João
Nakazato, Luciano
Bard, Emilie
Aguilar-Bultet, Lisandra
Vorimore, Fabien
Leonidovich Popov, Vsevolod
Moleta Colodel, Edson
Cabezas-Cruz, Alejandro
author_sort Moura de Aguiar, Daniel
collection PubMed
description The genus Ehrlichia is composed of tick-borne obligate intracellular gram-negative alphaproteobacteria of the family Anaplasmataceae. Ehrlichia includes important pathogens affecting canids (E. canis, E. chaffeensis, and E. ewingii), rodents (E. muris), and ruminants (E. ruminantium). Ehrlichia minasensis, an Ehrlichia closely related to E. canis, was initially reported in Canada and Brazil. This bacterium has now been reported in Pakistan, Malaysia, China, Ethiopia, South Africa, and the Mediterranean island of Corsica, suggesting that E. minasensis has a wide geographical distribution. Previously, E. minasensis was found to cause clinical ehrlichiosis in an experimentally infected calf. The type strain E. minasensis UFMG-EV was successfully isolated from Rhipicephalus microplus ticks and propagated in the tick embryonic cell line of Ixodes scapularis (IDE8). However, the isolation and propagation of E. minasensis strains from cattle has remained elusive. In this study, the E. minasensis strain Cuiabá was isolated from an eight-month-old male calf of Holstein breed that was naturally infected with the bacterium. The calf presented clinical signs and hematological parameters of bovine ehrlichiosis. The in vitro culture of the agent was established in the canine cell line DH82. Ehrlichial morulae were observed using light and electron microscopy within DH82 cells. Total DNA was extracted, and the full genome of the E. minasensis strain Cuiabá was sequenced. A core-genome-based phylogenetic tree of Ehrlichia spp. and Anaplasma spp. confirmed that E. minasensis is a sister taxa of E. canis. A comparison of functional categories among Ehrlichia showed that E. minasensis has significantly less genes in the ‘clustering-based subsystems’ category, which includes functionally coupled genes for which the functional attributes are not well understood. Results strongly suggest that E. minasensis is a novel pathogen infecting cattle. The epidemiology of this Ehrlichia deserves further attention because these bacteria could be an overlooked cause of tick-borne bovine ehrlichiosis, with a wide distribution.
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spelling pubmed-69210062019-12-24 Isolation and Characterization of a Novel Pathogenic Strain of Ehrlichia minasensis Moura de Aguiar, Daniel Pessoa Araújo Junior, João Nakazato, Luciano Bard, Emilie Aguilar-Bultet, Lisandra Vorimore, Fabien Leonidovich Popov, Vsevolod Moleta Colodel, Edson Cabezas-Cruz, Alejandro Microorganisms Article The genus Ehrlichia is composed of tick-borne obligate intracellular gram-negative alphaproteobacteria of the family Anaplasmataceae. Ehrlichia includes important pathogens affecting canids (E. canis, E. chaffeensis, and E. ewingii), rodents (E. muris), and ruminants (E. ruminantium). Ehrlichia minasensis, an Ehrlichia closely related to E. canis, was initially reported in Canada and Brazil. This bacterium has now been reported in Pakistan, Malaysia, China, Ethiopia, South Africa, and the Mediterranean island of Corsica, suggesting that E. minasensis has a wide geographical distribution. Previously, E. minasensis was found to cause clinical ehrlichiosis in an experimentally infected calf. The type strain E. minasensis UFMG-EV was successfully isolated from Rhipicephalus microplus ticks and propagated in the tick embryonic cell line of Ixodes scapularis (IDE8). However, the isolation and propagation of E. minasensis strains from cattle has remained elusive. In this study, the E. minasensis strain Cuiabá was isolated from an eight-month-old male calf of Holstein breed that was naturally infected with the bacterium. The calf presented clinical signs and hematological parameters of bovine ehrlichiosis. The in vitro culture of the agent was established in the canine cell line DH82. Ehrlichial morulae were observed using light and electron microscopy within DH82 cells. Total DNA was extracted, and the full genome of the E. minasensis strain Cuiabá was sequenced. A core-genome-based phylogenetic tree of Ehrlichia spp. and Anaplasma spp. confirmed that E. minasensis is a sister taxa of E. canis. A comparison of functional categories among Ehrlichia showed that E. minasensis has significantly less genes in the ‘clustering-based subsystems’ category, which includes functionally coupled genes for which the functional attributes are not well understood. Results strongly suggest that E. minasensis is a novel pathogen infecting cattle. The epidemiology of this Ehrlichia deserves further attention because these bacteria could be an overlooked cause of tick-borne bovine ehrlichiosis, with a wide distribution. MDPI 2019-11-05 /pmc/articles/PMC6921006/ /pubmed/31694172 http://dx.doi.org/10.3390/microorganisms7110528 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moura de Aguiar, Daniel
Pessoa Araújo Junior, João
Nakazato, Luciano
Bard, Emilie
Aguilar-Bultet, Lisandra
Vorimore, Fabien
Leonidovich Popov, Vsevolod
Moleta Colodel, Edson
Cabezas-Cruz, Alejandro
Isolation and Characterization of a Novel Pathogenic Strain of Ehrlichia minasensis
title Isolation and Characterization of a Novel Pathogenic Strain of Ehrlichia minasensis
title_full Isolation and Characterization of a Novel Pathogenic Strain of Ehrlichia minasensis
title_fullStr Isolation and Characterization of a Novel Pathogenic Strain of Ehrlichia minasensis
title_full_unstemmed Isolation and Characterization of a Novel Pathogenic Strain of Ehrlichia minasensis
title_short Isolation and Characterization of a Novel Pathogenic Strain of Ehrlichia minasensis
title_sort isolation and characterization of a novel pathogenic strain of ehrlichia minasensis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921006/
https://www.ncbi.nlm.nih.gov/pubmed/31694172
http://dx.doi.org/10.3390/microorganisms7110528
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