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Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats

Therapeutic approaches for the treatment of dyslipidemia and atherosclerosis have radically changed in recent decades. Part of this advance undeniably stems from basic biomedical research that has provided a better understanding and identification of new therapeutic targets. The aim of this work was...

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Autores principales: Guarnier, Lucas Pires, Romão, Paulo Vitor Moreira, Palozi, Rhanany Alan Calloi, Silva, Aniely Oliveira, Lorençone, Bethânia Rosa, Marques, Aline Aparecida Macedo, dos Santos, Ariany Carvalho, Souza, Roosevelt Isaias Carvalho, Souza, Karine Delgado, Lourenço, Emerson Luiz Botelho, Gasparotto Junior, Arquimedes
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921007/
https://www.ncbi.nlm.nih.gov/pubmed/31671756
http://dx.doi.org/10.3390/biom9110664
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author Guarnier, Lucas Pires
Romão, Paulo Vitor Moreira
Palozi, Rhanany Alan Calloi
Silva, Aniely Oliveira
Lorençone, Bethânia Rosa
Marques, Aline Aparecida Macedo
dos Santos, Ariany Carvalho
Souza, Roosevelt Isaias Carvalho
Souza, Karine Delgado
Lourenço, Emerson Luiz Botelho
Gasparotto Junior, Arquimedes
author_facet Guarnier, Lucas Pires
Romão, Paulo Vitor Moreira
Palozi, Rhanany Alan Calloi
Silva, Aniely Oliveira
Lorençone, Bethânia Rosa
Marques, Aline Aparecida Macedo
dos Santos, Ariany Carvalho
Souza, Roosevelt Isaias Carvalho
Souza, Karine Delgado
Lourenço, Emerson Luiz Botelho
Gasparotto Junior, Arquimedes
author_sort Guarnier, Lucas Pires
collection PubMed
description Therapeutic approaches for the treatment of dyslipidemia and atherosclerosis have radically changed in recent decades. Part of this advance undeniably stems from basic biomedical research that has provided a better understanding and identification of new therapeutic targets. The aim of this work was to develop a model to induce atherogenesis and hepato-renal impairment in female Wistar rats. The following groups received the respective treatments for 60 days: control animals, non-ovariectomized rats that received an atherogenic diet (NEAD), ovariectomized rats that received an atherogenic diet (NOAD), non-ovariectomized rats that received an atherogenic diet and oral Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME; LEAD), and ovariectomized rats that received an atherogenic diet and oral l-NAME (LOAD). Animals in the NEAD, NOAD, LEAD, and LOAD groups also received methimazole and cholecalciferol daily. Urinary, biochemical, hemodynamic, and electrocardiographic parameters and renal function were assessed. Samples of the liver, heart, kidney, and arteries were collected to investigate redox status and perform histopathological analyses. All of the groups developed dyslipidemia and hepatic steatosis. Only the NEAD group developed arterial lesions that were compatible with fatty streaks. Renal function was significantly impaired in the LEAD and NOAD groups. These results indicate a viable alternative to induce atherogenesis and hepato-renal impairment in female rats.
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spelling pubmed-69210072019-12-24 Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats Guarnier, Lucas Pires Romão, Paulo Vitor Moreira Palozi, Rhanany Alan Calloi Silva, Aniely Oliveira Lorençone, Bethânia Rosa Marques, Aline Aparecida Macedo dos Santos, Ariany Carvalho Souza, Roosevelt Isaias Carvalho Souza, Karine Delgado Lourenço, Emerson Luiz Botelho Gasparotto Junior, Arquimedes Biomolecules Article Therapeutic approaches for the treatment of dyslipidemia and atherosclerosis have radically changed in recent decades. Part of this advance undeniably stems from basic biomedical research that has provided a better understanding and identification of new therapeutic targets. The aim of this work was to develop a model to induce atherogenesis and hepato-renal impairment in female Wistar rats. The following groups received the respective treatments for 60 days: control animals, non-ovariectomized rats that received an atherogenic diet (NEAD), ovariectomized rats that received an atherogenic diet (NOAD), non-ovariectomized rats that received an atherogenic diet and oral Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME; LEAD), and ovariectomized rats that received an atherogenic diet and oral l-NAME (LOAD). Animals in the NEAD, NOAD, LEAD, and LOAD groups also received methimazole and cholecalciferol daily. Urinary, biochemical, hemodynamic, and electrocardiographic parameters and renal function were assessed. Samples of the liver, heart, kidney, and arteries were collected to investigate redox status and perform histopathological analyses. All of the groups developed dyslipidemia and hepatic steatosis. Only the NEAD group developed arterial lesions that were compatible with fatty streaks. Renal function was significantly impaired in the LEAD and NOAD groups. These results indicate a viable alternative to induce atherogenesis and hepato-renal impairment in female rats. MDPI 2019-10-29 /pmc/articles/PMC6921007/ /pubmed/31671756 http://dx.doi.org/10.3390/biom9110664 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guarnier, Lucas Pires
Romão, Paulo Vitor Moreira
Palozi, Rhanany Alan Calloi
Silva, Aniely Oliveira
Lorençone, Bethânia Rosa
Marques, Aline Aparecida Macedo
dos Santos, Ariany Carvalho
Souza, Roosevelt Isaias Carvalho
Souza, Karine Delgado
Lourenço, Emerson Luiz Botelho
Gasparotto Junior, Arquimedes
Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats
title Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats
title_full Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats
title_fullStr Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats
title_full_unstemmed Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats
title_short Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats
title_sort development of a predictive model to induce atherogenesis and hepato-renal impairment in female rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921007/
https://www.ncbi.nlm.nih.gov/pubmed/31671756
http://dx.doi.org/10.3390/biom9110664
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