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Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats
Therapeutic approaches for the treatment of dyslipidemia and atherosclerosis have radically changed in recent decades. Part of this advance undeniably stems from basic biomedical research that has provided a better understanding and identification of new therapeutic targets. The aim of this work was...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921007/ https://www.ncbi.nlm.nih.gov/pubmed/31671756 http://dx.doi.org/10.3390/biom9110664 |
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author | Guarnier, Lucas Pires Romão, Paulo Vitor Moreira Palozi, Rhanany Alan Calloi Silva, Aniely Oliveira Lorençone, Bethânia Rosa Marques, Aline Aparecida Macedo dos Santos, Ariany Carvalho Souza, Roosevelt Isaias Carvalho Souza, Karine Delgado Lourenço, Emerson Luiz Botelho Gasparotto Junior, Arquimedes |
author_facet | Guarnier, Lucas Pires Romão, Paulo Vitor Moreira Palozi, Rhanany Alan Calloi Silva, Aniely Oliveira Lorençone, Bethânia Rosa Marques, Aline Aparecida Macedo dos Santos, Ariany Carvalho Souza, Roosevelt Isaias Carvalho Souza, Karine Delgado Lourenço, Emerson Luiz Botelho Gasparotto Junior, Arquimedes |
author_sort | Guarnier, Lucas Pires |
collection | PubMed |
description | Therapeutic approaches for the treatment of dyslipidemia and atherosclerosis have radically changed in recent decades. Part of this advance undeniably stems from basic biomedical research that has provided a better understanding and identification of new therapeutic targets. The aim of this work was to develop a model to induce atherogenesis and hepato-renal impairment in female Wistar rats. The following groups received the respective treatments for 60 days: control animals, non-ovariectomized rats that received an atherogenic diet (NEAD), ovariectomized rats that received an atherogenic diet (NOAD), non-ovariectomized rats that received an atherogenic diet and oral Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME; LEAD), and ovariectomized rats that received an atherogenic diet and oral l-NAME (LOAD). Animals in the NEAD, NOAD, LEAD, and LOAD groups also received methimazole and cholecalciferol daily. Urinary, biochemical, hemodynamic, and electrocardiographic parameters and renal function were assessed. Samples of the liver, heart, kidney, and arteries were collected to investigate redox status and perform histopathological analyses. All of the groups developed dyslipidemia and hepatic steatosis. Only the NEAD group developed arterial lesions that were compatible with fatty streaks. Renal function was significantly impaired in the LEAD and NOAD groups. These results indicate a viable alternative to induce atherogenesis and hepato-renal impairment in female rats. |
format | Online Article Text |
id | pubmed-6921007 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69210072019-12-24 Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats Guarnier, Lucas Pires Romão, Paulo Vitor Moreira Palozi, Rhanany Alan Calloi Silva, Aniely Oliveira Lorençone, Bethânia Rosa Marques, Aline Aparecida Macedo dos Santos, Ariany Carvalho Souza, Roosevelt Isaias Carvalho Souza, Karine Delgado Lourenço, Emerson Luiz Botelho Gasparotto Junior, Arquimedes Biomolecules Article Therapeutic approaches for the treatment of dyslipidemia and atherosclerosis have radically changed in recent decades. Part of this advance undeniably stems from basic biomedical research that has provided a better understanding and identification of new therapeutic targets. The aim of this work was to develop a model to induce atherogenesis and hepato-renal impairment in female Wistar rats. The following groups received the respective treatments for 60 days: control animals, non-ovariectomized rats that received an atherogenic diet (NEAD), ovariectomized rats that received an atherogenic diet (NOAD), non-ovariectomized rats that received an atherogenic diet and oral Nω-nitro-l-arginine methyl ester hydrochloride (l-NAME; LEAD), and ovariectomized rats that received an atherogenic diet and oral l-NAME (LOAD). Animals in the NEAD, NOAD, LEAD, and LOAD groups also received methimazole and cholecalciferol daily. Urinary, biochemical, hemodynamic, and electrocardiographic parameters and renal function were assessed. Samples of the liver, heart, kidney, and arteries were collected to investigate redox status and perform histopathological analyses. All of the groups developed dyslipidemia and hepatic steatosis. Only the NEAD group developed arterial lesions that were compatible with fatty streaks. Renal function was significantly impaired in the LEAD and NOAD groups. These results indicate a viable alternative to induce atherogenesis and hepato-renal impairment in female rats. MDPI 2019-10-29 /pmc/articles/PMC6921007/ /pubmed/31671756 http://dx.doi.org/10.3390/biom9110664 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Guarnier, Lucas Pires Romão, Paulo Vitor Moreira Palozi, Rhanany Alan Calloi Silva, Aniely Oliveira Lorençone, Bethânia Rosa Marques, Aline Aparecida Macedo dos Santos, Ariany Carvalho Souza, Roosevelt Isaias Carvalho Souza, Karine Delgado Lourenço, Emerson Luiz Botelho Gasparotto Junior, Arquimedes Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats |
title | Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats |
title_full | Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats |
title_fullStr | Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats |
title_full_unstemmed | Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats |
title_short | Development of a Predictive Model to Induce Atherogenesis and Hepato-Renal Impairment in Female Rats |
title_sort | development of a predictive model to induce atherogenesis and hepato-renal impairment in female rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921007/ https://www.ncbi.nlm.nih.gov/pubmed/31671756 http://dx.doi.org/10.3390/biom9110664 |
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