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Functional Characterization and Structural Analysis of NADH Oxidase Mutants from Thermus thermophilus HB27: Role of Residues 166, 174, and 194 in the Catalytic Properties and Thermostability

The Thermus thermophilus strain HB27 NADH-oxidase (Tt27-NOX) catalyzes the oxidation of nicotinamide adenine dinucleotide (NAD(P)H) by reducing molecular oxygen to hydrogen peroxide in a two-electron transfer mechanism. Surprisingly, Tt27-NOX showed significant differences in catalytic properties co...

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Detalles Bibliográficos
Autores principales:	Rocha-Martin, Javier, Sánchez-Murcia, Pedro A., López-Gallego, Fernando, Hidalgo, Aurelio, Berenguer, José, Guisan, José M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921046/ https://www.ncbi.nlm.nih.gov/pubmed/31683638 http://dx.doi.org/10.3390/microorganisms7110515

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