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Development and validation of a high-performance thin-layer chromatographic method for the quantitative analysis of vitexin in Passiflora foetida herbal formulations

The aim of this study is the development of validated HPTLC method for the quantification of vitexin from Passiflora foetida commercial herbal formulations. The developed method was validated, in accordance with ICH guidelines for precision, accuracy, specificity and robustness. The plate was develo...

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Autores principales: Foudah, Ahmed Ibrahim, Alam, Prawez, Kamal, Y.T., Alqasoumi, Saleh Ibrahim, Alqarni, Mohammed Hamed, Ross, Samir A., Yusufoglu, Hasan Soliman
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921218/
https://www.ncbi.nlm.nih.gov/pubmed/31885475
http://dx.doi.org/10.1016/j.jsps.2019.09.012
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author Foudah, Ahmed Ibrahim
Alam, Prawez
Kamal, Y.T.
Alqasoumi, Saleh Ibrahim
Alqarni, Mohammed Hamed
Ross, Samir A.
Yusufoglu, Hasan Soliman
author_facet Foudah, Ahmed Ibrahim
Alam, Prawez
Kamal, Y.T.
Alqasoumi, Saleh Ibrahim
Alqarni, Mohammed Hamed
Ross, Samir A.
Yusufoglu, Hasan Soliman
author_sort Foudah, Ahmed Ibrahim
collection PubMed
description The aim of this study is the development of validated HPTLC method for the quantification of vitexin from Passiflora foetida commercial herbal formulations. The developed method was validated, in accordance with ICH guidelines for precision, accuracy, specificity and robustness. The plate was developed using ethyl acetate:methanol:water:formic acid 30:4:2:1(%, v/v/v/v) on 20 × 10 cm glass coated silica gel 60 F(254) plates and the developed plate was scanned and quantified densitometrically at λ = 340 nm. Linear regression analysis revealed a good linear relationship between peak area and amount of vitexin in the range of 100–700 ng/spot. The amount of vitexin in nine commercial herbal formulations was successfully quantified by the developed HPTLC method. The developed and validated high performance thin layer chromatographic method offers a new sensitive and reliable tool for quantification of vitexinin in various herbal formulations containing Passiflora foetida.
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spelling pubmed-69212182019-12-27 Development and validation of a high-performance thin-layer chromatographic method for the quantitative analysis of vitexin in Passiflora foetida herbal formulations Foudah, Ahmed Ibrahim Alam, Prawez Kamal, Y.T. Alqasoumi, Saleh Ibrahim Alqarni, Mohammed Hamed Ross, Samir A. Yusufoglu, Hasan Soliman Saudi Pharm J Article The aim of this study is the development of validated HPTLC method for the quantification of vitexin from Passiflora foetida commercial herbal formulations. The developed method was validated, in accordance with ICH guidelines for precision, accuracy, specificity and robustness. The plate was developed using ethyl acetate:methanol:water:formic acid 30:4:2:1(%, v/v/v/v) on 20 × 10 cm glass coated silica gel 60 F(254) plates and the developed plate was scanned and quantified densitometrically at λ = 340 nm. Linear regression analysis revealed a good linear relationship between peak area and amount of vitexin in the range of 100–700 ng/spot. The amount of vitexin in nine commercial herbal formulations was successfully quantified by the developed HPTLC method. The developed and validated high performance thin layer chromatographic method offers a new sensitive and reliable tool for quantification of vitexinin in various herbal formulations containing Passiflora foetida. Elsevier 2019-12 2019-11-08 /pmc/articles/PMC6921218/ /pubmed/31885475 http://dx.doi.org/10.1016/j.jsps.2019.09.012 Text en © 2019 The Author(s) http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Foudah, Ahmed Ibrahim
Alam, Prawez
Kamal, Y.T.
Alqasoumi, Saleh Ibrahim
Alqarni, Mohammed Hamed
Ross, Samir A.
Yusufoglu, Hasan Soliman
Development and validation of a high-performance thin-layer chromatographic method for the quantitative analysis of vitexin in Passiflora foetida herbal formulations
title Development and validation of a high-performance thin-layer chromatographic method for the quantitative analysis of vitexin in Passiflora foetida herbal formulations
title_full Development and validation of a high-performance thin-layer chromatographic method for the quantitative analysis of vitexin in Passiflora foetida herbal formulations
title_fullStr Development and validation of a high-performance thin-layer chromatographic method for the quantitative analysis of vitexin in Passiflora foetida herbal formulations
title_full_unstemmed Development and validation of a high-performance thin-layer chromatographic method for the quantitative analysis of vitexin in Passiflora foetida herbal formulations
title_short Development and validation of a high-performance thin-layer chromatographic method for the quantitative analysis of vitexin in Passiflora foetida herbal formulations
title_sort development and validation of a high-performance thin-layer chromatographic method for the quantitative analysis of vitexin in passiflora foetida herbal formulations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921218/
https://www.ncbi.nlm.nih.gov/pubmed/31885475
http://dx.doi.org/10.1016/j.jsps.2019.09.012
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