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YAP as a key regulator of adipo-osteogenic differentiation in human MSCs
BACKGROUND: Mesenchymal stem cells (MSCs) are multipotent stem cells that are able to differentiate into several cell types, including cartilage, fat, and bone. As a common progenitor, MSC differentiation has to be tightly regulated to maintain the balance of their differentiation commitment. It has...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921580/ https://www.ncbi.nlm.nih.gov/pubmed/31852542 http://dx.doi.org/10.1186/s13287-019-1494-4 |
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author | Lorthongpanich, Chanchao Thumanu, Kanjana Tangkiettrakul, Kantpitchar Jiamvoraphong, Nittaya Laowtammathron, Chuti Damkham, Nattaya U-pratya, Yaowalak Issaragrisil, Surapol |
author_facet | Lorthongpanich, Chanchao Thumanu, Kanjana Tangkiettrakul, Kantpitchar Jiamvoraphong, Nittaya Laowtammathron, Chuti Damkham, Nattaya U-pratya, Yaowalak Issaragrisil, Surapol |
author_sort | Lorthongpanich, Chanchao |
collection | PubMed |
description | BACKGROUND: Mesenchymal stem cells (MSCs) are multipotent stem cells that are able to differentiate into several cell types, including cartilage, fat, and bone. As a common progenitor, MSC differentiation has to be tightly regulated to maintain the balance of their differentiation commitment. It has been reported that the decision process of MSCs into fat and bone cells is competing and reciprocal. Several factors have been suggested as critical factors that affect adipo-osteogenic decision, including melatonin and smad4. Yes-associated protein (YAP) is an important effector protein in the Hippo signaling pathway that acts as a transcriptional regulator by activating the transcription of the genes involved in cell proliferation and anti-apoptosis. The non-canonical role of YAP in regulating bone homeostasis by promoting osteogenesis and suppressing adipogenesis was recently demonstrated in a mouse model. However, it is unclear whether YAP is also crucial for modulating human MSC differentiation to fat and bone. METHODS: The expression level of YAP during MSC differentiation was modulated using pharmaceutical molecule and genetic experiments through gain- and loss-of-function approaches. RESULTS: We demonstrated for the first time that YAP has a non-canonical role in regulating the balance of adipo-osteogenic differentiation of human MSCs. The result from synchrotron radiation-based Fourier transform infrared (FTIR) microspectroscopy showed unique metabolic fingerprints generated from YAP-targeted differentiated cells that were clearly distinguished from non-manipulated control. CONCLUSIONS: These results, thus, identify YAP as an important effector protein that regulates human MSC differentiation to fat and bone and suggests the use of FTIR microspectroscopy as a promising technique in stem cell research. |
format | Online Article Text |
id | pubmed-6921580 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-69215802019-12-30 YAP as a key regulator of adipo-osteogenic differentiation in human MSCs Lorthongpanich, Chanchao Thumanu, Kanjana Tangkiettrakul, Kantpitchar Jiamvoraphong, Nittaya Laowtammathron, Chuti Damkham, Nattaya U-pratya, Yaowalak Issaragrisil, Surapol Stem Cell Res Ther Research BACKGROUND: Mesenchymal stem cells (MSCs) are multipotent stem cells that are able to differentiate into several cell types, including cartilage, fat, and bone. As a common progenitor, MSC differentiation has to be tightly regulated to maintain the balance of their differentiation commitment. It has been reported that the decision process of MSCs into fat and bone cells is competing and reciprocal. Several factors have been suggested as critical factors that affect adipo-osteogenic decision, including melatonin and smad4. Yes-associated protein (YAP) is an important effector protein in the Hippo signaling pathway that acts as a transcriptional regulator by activating the transcription of the genes involved in cell proliferation and anti-apoptosis. The non-canonical role of YAP in regulating bone homeostasis by promoting osteogenesis and suppressing adipogenesis was recently demonstrated in a mouse model. However, it is unclear whether YAP is also crucial for modulating human MSC differentiation to fat and bone. METHODS: The expression level of YAP during MSC differentiation was modulated using pharmaceutical molecule and genetic experiments through gain- and loss-of-function approaches. RESULTS: We demonstrated for the first time that YAP has a non-canonical role in regulating the balance of adipo-osteogenic differentiation of human MSCs. The result from synchrotron radiation-based Fourier transform infrared (FTIR) microspectroscopy showed unique metabolic fingerprints generated from YAP-targeted differentiated cells that were clearly distinguished from non-manipulated control. CONCLUSIONS: These results, thus, identify YAP as an important effector protein that regulates human MSC differentiation to fat and bone and suggests the use of FTIR microspectroscopy as a promising technique in stem cell research. BioMed Central 2019-12-18 /pmc/articles/PMC6921580/ /pubmed/31852542 http://dx.doi.org/10.1186/s13287-019-1494-4 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Lorthongpanich, Chanchao Thumanu, Kanjana Tangkiettrakul, Kantpitchar Jiamvoraphong, Nittaya Laowtammathron, Chuti Damkham, Nattaya U-pratya, Yaowalak Issaragrisil, Surapol YAP as a key regulator of adipo-osteogenic differentiation in human MSCs |
title | YAP as a key regulator of adipo-osteogenic differentiation in human MSCs |
title_full | YAP as a key regulator of adipo-osteogenic differentiation in human MSCs |
title_fullStr | YAP as a key regulator of adipo-osteogenic differentiation in human MSCs |
title_full_unstemmed | YAP as a key regulator of adipo-osteogenic differentiation in human MSCs |
title_short | YAP as a key regulator of adipo-osteogenic differentiation in human MSCs |
title_sort | yap as a key regulator of adipo-osteogenic differentiation in human mscs |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6921580/ https://www.ncbi.nlm.nih.gov/pubmed/31852542 http://dx.doi.org/10.1186/s13287-019-1494-4 |
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