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Dengue virus reduces AGPAT1 expression to alter phospholipids and enhance infection in Aedes aegypti

More than half of the world population is at risk of dengue virus (DENV) infection because of the global distribution of its mosquito vectors. DENV is an envelope virus that relies on host lipid membranes for its life-cycle. Here, we characterized how DENV hijacks the mosquito lipidome to identify t...

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Autores principales: Vial, Thomas, Tan, Wei-Lian, Wong Wei Xiang, Benjamin, Missé, Dorothée, Deharo, Eric, Marti, Guillaume, Pompon, Julien
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6922471/
https://www.ncbi.nlm.nih.gov/pubmed/31815960
http://dx.doi.org/10.1371/journal.ppat.1008199
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author Vial, Thomas
Tan, Wei-Lian
Wong Wei Xiang, Benjamin
Missé, Dorothée
Deharo, Eric
Marti, Guillaume
Pompon, Julien
author_facet Vial, Thomas
Tan, Wei-Lian
Wong Wei Xiang, Benjamin
Missé, Dorothée
Deharo, Eric
Marti, Guillaume
Pompon, Julien
author_sort Vial, Thomas
collection PubMed
description More than half of the world population is at risk of dengue virus (DENV) infection because of the global distribution of its mosquito vectors. DENV is an envelope virus that relies on host lipid membranes for its life-cycle. Here, we characterized how DENV hijacks the mosquito lipidome to identify targets for novel transmission-blocking interventions. To describe metabolic changes throughout the mosquito DENV cycle, we deployed a Liquid chromatography–high resolution mass spectrometry (LC-HRMS) workflow including spectral similarity annotation in cells, midguts and whole mosquitoes at different times post infection. We revealed a major aminophospholipid reconfiguration with an overall early increase, followed by a reduction later in the cycle. We phylogenetically characterized acylglycerolphosphate acyltransferase (AGPAT) enzyme isoforms to identify those that catalyze a rate-limiting step in phospholipid biogenesis, the acylation of lysophosphatidate to phosphatidate. We showed that DENV infection decreased AGPAT1, but did not alter AGPAT2 expression in cells, midguts and mosquitoes. Depletion of either AGPAT1 or AGPAT2 increased aminophospholipids and partially recapitulated DENV-induced reconfiguration before infection in vitro. However, only AGPAT1 depletion promoted infection by maintaining high aminophospholipid concentrations. In mosquitoes, AGPAT1 depletion also partially recapitulated DENV-induced aminophospholipid increase before infection and enhanced infection by maintaining high aminophospholipid concentrations. These results indicate that DENV inhibition of AGPAT1 expression promotes infection by increasing aminophospholipids, as observed in the mosquito’s early DENV cycle. Furthermore, in AGPAT1-depleted mosquitoes, we showed that enhanced infection was associated with increased consumption/redirection of aminophospholipids. Our study suggests that DENV regulates aminophospholipids, especially phosphatidylcholine and phosphatidylethanolamine, by inhibiting AGPAT1 expression to increase aminophospholipid availability for virus multiplication.
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spelling pubmed-69224712020-01-07 Dengue virus reduces AGPAT1 expression to alter phospholipids and enhance infection in Aedes aegypti Vial, Thomas Tan, Wei-Lian Wong Wei Xiang, Benjamin Missé, Dorothée Deharo, Eric Marti, Guillaume Pompon, Julien PLoS Pathog Research Article More than half of the world population is at risk of dengue virus (DENV) infection because of the global distribution of its mosquito vectors. DENV is an envelope virus that relies on host lipid membranes for its life-cycle. Here, we characterized how DENV hijacks the mosquito lipidome to identify targets for novel transmission-blocking interventions. To describe metabolic changes throughout the mosquito DENV cycle, we deployed a Liquid chromatography–high resolution mass spectrometry (LC-HRMS) workflow including spectral similarity annotation in cells, midguts and whole mosquitoes at different times post infection. We revealed a major aminophospholipid reconfiguration with an overall early increase, followed by a reduction later in the cycle. We phylogenetically characterized acylglycerolphosphate acyltransferase (AGPAT) enzyme isoforms to identify those that catalyze a rate-limiting step in phospholipid biogenesis, the acylation of lysophosphatidate to phosphatidate. We showed that DENV infection decreased AGPAT1, but did not alter AGPAT2 expression in cells, midguts and mosquitoes. Depletion of either AGPAT1 or AGPAT2 increased aminophospholipids and partially recapitulated DENV-induced reconfiguration before infection in vitro. However, only AGPAT1 depletion promoted infection by maintaining high aminophospholipid concentrations. In mosquitoes, AGPAT1 depletion also partially recapitulated DENV-induced aminophospholipid increase before infection and enhanced infection by maintaining high aminophospholipid concentrations. These results indicate that DENV inhibition of AGPAT1 expression promotes infection by increasing aminophospholipids, as observed in the mosquito’s early DENV cycle. Furthermore, in AGPAT1-depleted mosquitoes, we showed that enhanced infection was associated with increased consumption/redirection of aminophospholipids. Our study suggests that DENV regulates aminophospholipids, especially phosphatidylcholine and phosphatidylethanolamine, by inhibiting AGPAT1 expression to increase aminophospholipid availability for virus multiplication. Public Library of Science 2019-12-09 /pmc/articles/PMC6922471/ /pubmed/31815960 http://dx.doi.org/10.1371/journal.ppat.1008199 Text en © 2019 Vial et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Vial, Thomas
Tan, Wei-Lian
Wong Wei Xiang, Benjamin
Missé, Dorothée
Deharo, Eric
Marti, Guillaume
Pompon, Julien
Dengue virus reduces AGPAT1 expression to alter phospholipids and enhance infection in Aedes aegypti
title Dengue virus reduces AGPAT1 expression to alter phospholipids and enhance infection in Aedes aegypti
title_full Dengue virus reduces AGPAT1 expression to alter phospholipids and enhance infection in Aedes aegypti
title_fullStr Dengue virus reduces AGPAT1 expression to alter phospholipids and enhance infection in Aedes aegypti
title_full_unstemmed Dengue virus reduces AGPAT1 expression to alter phospholipids and enhance infection in Aedes aegypti
title_short Dengue virus reduces AGPAT1 expression to alter phospholipids and enhance infection in Aedes aegypti
title_sort dengue virus reduces agpat1 expression to alter phospholipids and enhance infection in aedes aegypti
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6922471/
https://www.ncbi.nlm.nih.gov/pubmed/31815960
http://dx.doi.org/10.1371/journal.ppat.1008199
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