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CRISPR interference‐mediated gene regulation in Pseudomonas putida KT2440

Targeted gene regulation is indispensable for reprogramming a cellular network to modulate a microbial phenotype. Here, we adopted the type II CRISPR interference (CRISPRi) system for simple and efficient regulation of target genes in Pseudomonas putida KT2440. A single CRISPRi plasmid was generated...

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Detalles Bibliográficos
Autores principales: Kim, Seong Keun, Yoon, Paul K., Kim, Soo‐Jung, Woo, Seung‐Gyun, Rha, Eugene, Lee, Hyewon, Yeom, Soo‐Jin, Kim, Haseong, Lee, Dae‐Hee, Lee, Seung‐Goo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6922533/
https://www.ncbi.nlm.nih.gov/pubmed/30793496
http://dx.doi.org/10.1111/1751-7915.13382