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Spinal cord motor neuron plasticity accompanies second‐degree burn injury and chronic pain
Burn injuries and associated complications present a major public health challenge. Many burn patients develop clinically intractable complications, including pain and other sensory disorders. Recent evidence has shown that dendritic spine neuropathology in spinal cord sensory and motor neurons acco...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923170/ https://www.ncbi.nlm.nih.gov/pubmed/31858746 http://dx.doi.org/10.14814/phy2.14288 |
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author | Patwa, Siraj Benson, Curtis A. Dyer, Lauren Olson, Kai‐Lan Bangalore, Lakshmi Hill, Myriam Waxman, Stephen G. Tan, Andrew M. |
author_facet | Patwa, Siraj Benson, Curtis A. Dyer, Lauren Olson, Kai‐Lan Bangalore, Lakshmi Hill, Myriam Waxman, Stephen G. Tan, Andrew M. |
author_sort | Patwa, Siraj |
collection | PubMed |
description | Burn injuries and associated complications present a major public health challenge. Many burn patients develop clinically intractable complications, including pain and other sensory disorders. Recent evidence has shown that dendritic spine neuropathology in spinal cord sensory and motor neurons accompanies central nervous system (CNS) or peripheral nervous system (PNS) trauma and disease. However, no research has investigated similar dendritic spine neuropathologies following a cutaneous thermal burn injury. In this retrospective investigation, we analyzed dendritic spine morphology and localization in alpha‐motor neurons innervating a burn‐injured area of the body (hind paw). To identify a molecular regulator of these dendritic spine changes, we further profiled motor neuron dendritic spines in adult mice treated with romidepsin, a clinically approved Pak1‐inhibitor, or vehicle control at two postburn time points: Day 6 immediately after treatment, or Day 10 following drug withdrawal. In control treated mice, we observed an overall increase in dendritic spine density, including structurally mature spines with mushroom‐shaped morphology. Pak1‐inhibitor treatment reduced injury‐induced changes to similar levels observed in animals without burn injury. The effectiveness of the Pak1‐inhibitor was durable, since normalized dendritic spine profiles remained as long as 4 days despite drug withdrawal. This study is the first report of evidence demonstrating that a second‐degree burn injury significantly affects motor neuron structure within the spinal cord. Furthermore, our results support the opportunity to study dendritic spine dysgenesis as a novel avenue to clarify the complexities of neurological disease following traumatic injury. |
format | Online Article Text |
id | pubmed-6923170 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-69231702019-12-30 Spinal cord motor neuron plasticity accompanies second‐degree burn injury and chronic pain Patwa, Siraj Benson, Curtis A. Dyer, Lauren Olson, Kai‐Lan Bangalore, Lakshmi Hill, Myriam Waxman, Stephen G. Tan, Andrew M. Physiol Rep Original Articles Burn injuries and associated complications present a major public health challenge. Many burn patients develop clinically intractable complications, including pain and other sensory disorders. Recent evidence has shown that dendritic spine neuropathology in spinal cord sensory and motor neurons accompanies central nervous system (CNS) or peripheral nervous system (PNS) trauma and disease. However, no research has investigated similar dendritic spine neuropathologies following a cutaneous thermal burn injury. In this retrospective investigation, we analyzed dendritic spine morphology and localization in alpha‐motor neurons innervating a burn‐injured area of the body (hind paw). To identify a molecular regulator of these dendritic spine changes, we further profiled motor neuron dendritic spines in adult mice treated with romidepsin, a clinically approved Pak1‐inhibitor, or vehicle control at two postburn time points: Day 6 immediately after treatment, or Day 10 following drug withdrawal. In control treated mice, we observed an overall increase in dendritic spine density, including structurally mature spines with mushroom‐shaped morphology. Pak1‐inhibitor treatment reduced injury‐induced changes to similar levels observed in animals without burn injury. The effectiveness of the Pak1‐inhibitor was durable, since normalized dendritic spine profiles remained as long as 4 days despite drug withdrawal. This study is the first report of evidence demonstrating that a second‐degree burn injury significantly affects motor neuron structure within the spinal cord. Furthermore, our results support the opportunity to study dendritic spine dysgenesis as a novel avenue to clarify the complexities of neurological disease following traumatic injury. John Wiley and Sons Inc. 2019-12-19 /pmc/articles/PMC6923170/ /pubmed/31858746 http://dx.doi.org/10.14814/phy2.14288 Text en © 2019 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Patwa, Siraj Benson, Curtis A. Dyer, Lauren Olson, Kai‐Lan Bangalore, Lakshmi Hill, Myriam Waxman, Stephen G. Tan, Andrew M. Spinal cord motor neuron plasticity accompanies second‐degree burn injury and chronic pain |
title | Spinal cord motor neuron plasticity accompanies second‐degree burn injury and chronic pain |
title_full | Spinal cord motor neuron plasticity accompanies second‐degree burn injury and chronic pain |
title_fullStr | Spinal cord motor neuron plasticity accompanies second‐degree burn injury and chronic pain |
title_full_unstemmed | Spinal cord motor neuron plasticity accompanies second‐degree burn injury and chronic pain |
title_short | Spinal cord motor neuron plasticity accompanies second‐degree burn injury and chronic pain |
title_sort | spinal cord motor neuron plasticity accompanies second‐degree burn injury and chronic pain |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923170/ https://www.ncbi.nlm.nih.gov/pubmed/31858746 http://dx.doi.org/10.14814/phy2.14288 |
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