Cargando…
Comparison of Escherichia coli surface attachment methods for single-cell microscopy
For in vivo, single-cell imaging bacterial cells are commonly immobilised via physical confinement or surface attachment. Different surface attachment methods have been used both for atomic force and optical microscopy (including super resolution), and some have been reported to affect bacterial phy...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923479/ https://www.ncbi.nlm.nih.gov/pubmed/31857669 http://dx.doi.org/10.1038/s41598-019-55798-0 |
_version_ | 1783481543385153536 |
---|---|
author | Wang, Yao-Kuan Krasnopeeva, Ekaterina Lin, Ssu-Yuan Bai, Fan Pilizota, Teuta Lo, Chien-Jung |
author_facet | Wang, Yao-Kuan Krasnopeeva, Ekaterina Lin, Ssu-Yuan Bai, Fan Pilizota, Teuta Lo, Chien-Jung |
author_sort | Wang, Yao-Kuan |
collection | PubMed |
description | For in vivo, single-cell imaging bacterial cells are commonly immobilised via physical confinement or surface attachment. Different surface attachment methods have been used both for atomic force and optical microscopy (including super resolution), and some have been reported to affect bacterial physiology. However, a systematic comparison of the effects these attachment methods have on the bacterial physiology is lacking. Here we present such a comparison for bacterium Escherichia coli, and assess the growth rate, size and intracellular pH of cells growing attached to different, commonly used, surfaces. We demonstrate that E. coli grow at the same rate, length and internal pH on all the tested surfaces when in the same growth medium. The result suggests that tested attachment methods can be used interchangeably when studying E. coli physiology. |
format | Online Article Text |
id | pubmed-6923479 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-69234792019-12-23 Comparison of Escherichia coli surface attachment methods for single-cell microscopy Wang, Yao-Kuan Krasnopeeva, Ekaterina Lin, Ssu-Yuan Bai, Fan Pilizota, Teuta Lo, Chien-Jung Sci Rep Article For in vivo, single-cell imaging bacterial cells are commonly immobilised via physical confinement or surface attachment. Different surface attachment methods have been used both for atomic force and optical microscopy (including super resolution), and some have been reported to affect bacterial physiology. However, a systematic comparison of the effects these attachment methods have on the bacterial physiology is lacking. Here we present such a comparison for bacterium Escherichia coli, and assess the growth rate, size and intracellular pH of cells growing attached to different, commonly used, surfaces. We demonstrate that E. coli grow at the same rate, length and internal pH on all the tested surfaces when in the same growth medium. The result suggests that tested attachment methods can be used interchangeably when studying E. coli physiology. Nature Publishing Group UK 2019-12-19 /pmc/articles/PMC6923479/ /pubmed/31857669 http://dx.doi.org/10.1038/s41598-019-55798-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Wang, Yao-Kuan Krasnopeeva, Ekaterina Lin, Ssu-Yuan Bai, Fan Pilizota, Teuta Lo, Chien-Jung Comparison of Escherichia coli surface attachment methods for single-cell microscopy |
title | Comparison of Escherichia coli surface attachment methods for single-cell microscopy |
title_full | Comparison of Escherichia coli surface attachment methods for single-cell microscopy |
title_fullStr | Comparison of Escherichia coli surface attachment methods for single-cell microscopy |
title_full_unstemmed | Comparison of Escherichia coli surface attachment methods for single-cell microscopy |
title_short | Comparison of Escherichia coli surface attachment methods for single-cell microscopy |
title_sort | comparison of escherichia coli surface attachment methods for single-cell microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923479/ https://www.ncbi.nlm.nih.gov/pubmed/31857669 http://dx.doi.org/10.1038/s41598-019-55798-0 |
work_keys_str_mv | AT wangyaokuan comparisonofescherichiacolisurfaceattachmentmethodsforsinglecellmicroscopy AT krasnopeevaekaterina comparisonofescherichiacolisurfaceattachmentmethodsforsinglecellmicroscopy AT linssuyuan comparisonofescherichiacolisurfaceattachmentmethodsforsinglecellmicroscopy AT baifan comparisonofescherichiacolisurfaceattachmentmethodsforsinglecellmicroscopy AT pilizotateuta comparisonofescherichiacolisurfaceattachmentmethodsforsinglecellmicroscopy AT lochienjung comparisonofescherichiacolisurfaceattachmentmethodsforsinglecellmicroscopy |