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Comparison of Escherichia coli surface attachment methods for single-cell microscopy

For in vivo, single-cell imaging bacterial cells are commonly immobilised via physical confinement or surface attachment. Different surface attachment methods have been used both for atomic force and optical microscopy (including super resolution), and some have been reported to affect bacterial phy...

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Autores principales: Wang, Yao-Kuan, Krasnopeeva, Ekaterina, Lin, Ssu-Yuan, Bai, Fan, Pilizota, Teuta, Lo, Chien-Jung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923479/
https://www.ncbi.nlm.nih.gov/pubmed/31857669
http://dx.doi.org/10.1038/s41598-019-55798-0
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author Wang, Yao-Kuan
Krasnopeeva, Ekaterina
Lin, Ssu-Yuan
Bai, Fan
Pilizota, Teuta
Lo, Chien-Jung
author_facet Wang, Yao-Kuan
Krasnopeeva, Ekaterina
Lin, Ssu-Yuan
Bai, Fan
Pilizota, Teuta
Lo, Chien-Jung
author_sort Wang, Yao-Kuan
collection PubMed
description For in vivo, single-cell imaging bacterial cells are commonly immobilised via physical confinement or surface attachment. Different surface attachment methods have been used both for atomic force and optical microscopy (including super resolution), and some have been reported to affect bacterial physiology. However, a systematic comparison of the effects these attachment methods have on the bacterial physiology is lacking. Here we present such a comparison for bacterium Escherichia coli, and assess the growth rate, size and intracellular pH of cells growing attached to different, commonly used, surfaces. We demonstrate that E. coli grow at the same rate, length and internal pH on all the tested surfaces when in the same growth medium. The result suggests that tested attachment methods can be used interchangeably when studying E. coli physiology.
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spelling pubmed-69234792019-12-23 Comparison of Escherichia coli surface attachment methods for single-cell microscopy Wang, Yao-Kuan Krasnopeeva, Ekaterina Lin, Ssu-Yuan Bai, Fan Pilizota, Teuta Lo, Chien-Jung Sci Rep Article For in vivo, single-cell imaging bacterial cells are commonly immobilised via physical confinement or surface attachment. Different surface attachment methods have been used both for atomic force and optical microscopy (including super resolution), and some have been reported to affect bacterial physiology. However, a systematic comparison of the effects these attachment methods have on the bacterial physiology is lacking. Here we present such a comparison for bacterium Escherichia coli, and assess the growth rate, size and intracellular pH of cells growing attached to different, commonly used, surfaces. We demonstrate that E. coli grow at the same rate, length and internal pH on all the tested surfaces when in the same growth medium. The result suggests that tested attachment methods can be used interchangeably when studying E. coli physiology. Nature Publishing Group UK 2019-12-19 /pmc/articles/PMC6923479/ /pubmed/31857669 http://dx.doi.org/10.1038/s41598-019-55798-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wang, Yao-Kuan
Krasnopeeva, Ekaterina
Lin, Ssu-Yuan
Bai, Fan
Pilizota, Teuta
Lo, Chien-Jung
Comparison of Escherichia coli surface attachment methods for single-cell microscopy
title Comparison of Escherichia coli surface attachment methods for single-cell microscopy
title_full Comparison of Escherichia coli surface attachment methods for single-cell microscopy
title_fullStr Comparison of Escherichia coli surface attachment methods for single-cell microscopy
title_full_unstemmed Comparison of Escherichia coli surface attachment methods for single-cell microscopy
title_short Comparison of Escherichia coli surface attachment methods for single-cell microscopy
title_sort comparison of escherichia coli surface attachment methods for single-cell microscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923479/
https://www.ncbi.nlm.nih.gov/pubmed/31857669
http://dx.doi.org/10.1038/s41598-019-55798-0
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