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The preventive effect of Apocynum venetum polyphenols on D-galactose-induced oxidative stress in mice
Apocynum venetum is a traditional medicine that is rich in polyphenols. Apocynum venetum polyphenol extract (AVP) contains the active substances neochlorogenic acid, chlorogenic acid, rutin, isoquercitrin, astragaloside and rosmarinic acid. In the present study, the preventive effect of AVP against...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923744/ https://www.ncbi.nlm.nih.gov/pubmed/31897099 http://dx.doi.org/10.3892/etm.2019.8261 |
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author | Guo, Huan Kuang, Zhiping Zhang, Jing Zhao, Xin Pu, Ping Yan, Junfeng |
author_facet | Guo, Huan Kuang, Zhiping Zhang, Jing Zhao, Xin Pu, Ping Yan, Junfeng |
author_sort | Guo, Huan |
collection | PubMed |
description | Apocynum venetum is a traditional medicine that is rich in polyphenols. Apocynum venetum polyphenol extract (AVP) contains the active substances neochlorogenic acid, chlorogenic acid, rutin, isoquercitrin, astragaloside and rosmarinic acid. In the present study, the preventive effect of AVP against D-galactose-induced oxidative stress was studied in a mouse model. The sera, skin, livers and spleens of mice were examined using hematoxylin and eosin staining, reverse transcription-quantitative PCR and western blot analysis. The biochemical results showed that AVP improved the thymus, brain, heart, liver, spleen and kidney indices in a mouse model of oxidative stress. AVP was also able to reverse the reduction in levels of superoxide dismutase (SOD), glutathione peroxidase and glutathione, and increased the levels of nitric oxide and malondialdehyde identified in the serum, liver, spleen and brain of mice exposed to oxidative stress. Pathological observations confirmed that AVP could inhibit oxidative damage to the skin, liver and spleen of mice caused by D-galactose. Further molecular biological experiments also demonstrated that AVP increased the expression of neuronal nitric oxide synthase, endothelial nitric oxide synthase, Cu/Zn-SOD, Mn-SOD, catalase, heme oxygenase-1, nuclear factor-erythroid 2-related factor 2, γ-glutamylcysteine synthetase and NAD(P)H quinone dehydrogenase 1 and reduced the expression of inducible nitric oxide synthase in the liver and spleen of treated mice compared to controls. Notably, the preventive effect of AVP against D-galactose-induced oxidative damage in mice was better than that of the confirmed antioxidant vitamin C. In conclusion, AVP exhibited an antioxidant effect and the AVP-rich Apocynum venetum may be considered a plant resource with potential antioxidative benefits. |
format | Online Article Text |
id | pubmed-6923744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-69237442020-01-02 The preventive effect of Apocynum venetum polyphenols on D-galactose-induced oxidative stress in mice Guo, Huan Kuang, Zhiping Zhang, Jing Zhao, Xin Pu, Ping Yan, Junfeng Exp Ther Med Articles Apocynum venetum is a traditional medicine that is rich in polyphenols. Apocynum venetum polyphenol extract (AVP) contains the active substances neochlorogenic acid, chlorogenic acid, rutin, isoquercitrin, astragaloside and rosmarinic acid. In the present study, the preventive effect of AVP against D-galactose-induced oxidative stress was studied in a mouse model. The sera, skin, livers and spleens of mice were examined using hematoxylin and eosin staining, reverse transcription-quantitative PCR and western blot analysis. The biochemical results showed that AVP improved the thymus, brain, heart, liver, spleen and kidney indices in a mouse model of oxidative stress. AVP was also able to reverse the reduction in levels of superoxide dismutase (SOD), glutathione peroxidase and glutathione, and increased the levels of nitric oxide and malondialdehyde identified in the serum, liver, spleen and brain of mice exposed to oxidative stress. Pathological observations confirmed that AVP could inhibit oxidative damage to the skin, liver and spleen of mice caused by D-galactose. Further molecular biological experiments also demonstrated that AVP increased the expression of neuronal nitric oxide synthase, endothelial nitric oxide synthase, Cu/Zn-SOD, Mn-SOD, catalase, heme oxygenase-1, nuclear factor-erythroid 2-related factor 2, γ-glutamylcysteine synthetase and NAD(P)H quinone dehydrogenase 1 and reduced the expression of inducible nitric oxide synthase in the liver and spleen of treated mice compared to controls. Notably, the preventive effect of AVP against D-galactose-induced oxidative damage in mice was better than that of the confirmed antioxidant vitamin C. In conclusion, AVP exhibited an antioxidant effect and the AVP-rich Apocynum venetum may be considered a plant resource with potential antioxidative benefits. D.A. Spandidos 2020-01 2019-11-29 /pmc/articles/PMC6923744/ /pubmed/31897099 http://dx.doi.org/10.3892/etm.2019.8261 Text en Copyright: © Guo et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Guo, Huan Kuang, Zhiping Zhang, Jing Zhao, Xin Pu, Ping Yan, Junfeng The preventive effect of Apocynum venetum polyphenols on D-galactose-induced oxidative stress in mice |
title | The preventive effect of Apocynum venetum polyphenols on D-galactose-induced oxidative stress in mice |
title_full | The preventive effect of Apocynum venetum polyphenols on D-galactose-induced oxidative stress in mice |
title_fullStr | The preventive effect of Apocynum venetum polyphenols on D-galactose-induced oxidative stress in mice |
title_full_unstemmed | The preventive effect of Apocynum venetum polyphenols on D-galactose-induced oxidative stress in mice |
title_short | The preventive effect of Apocynum venetum polyphenols on D-galactose-induced oxidative stress in mice |
title_sort | preventive effect of apocynum venetum polyphenols on d-galactose-induced oxidative stress in mice |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923744/ https://www.ncbi.nlm.nih.gov/pubmed/31897099 http://dx.doi.org/10.3892/etm.2019.8261 |
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