Cargando…

Anti-inflammatory, analgesic and acute toxicity effects of fermented soybean

BACKGROUND: Tempeh is a widely known fermented soybean that contains elevated level of bioactive contents. Our previous study has shown that anaerobic fermented Nutrient Enriched Soybean Tempeh (NESTE) with increase amino acid and antioxidant levels possessed better hepatoprotective effect than raw...

Descripción completa

Detalles Bibliográficos
Autores principales: Yusof, Hamidah Mohd, Ali, Norlaily Mohd, Yeap, Swee Keong, Ho, Wan Yong, Beh, Boon Kee, Koh, Soo Peng, Long, Kamariah, Alitheen, Noorjahan Banu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923968/
https://www.ncbi.nlm.nih.gov/pubmed/31856816
http://dx.doi.org/10.1186/s12906-019-2791-2
_version_ 1783481635043278848
author Yusof, Hamidah Mohd
Ali, Norlaily Mohd
Yeap, Swee Keong
Ho, Wan Yong
Beh, Boon Kee
Koh, Soo Peng
Long, Kamariah
Alitheen, Noorjahan Banu
author_facet Yusof, Hamidah Mohd
Ali, Norlaily Mohd
Yeap, Swee Keong
Ho, Wan Yong
Beh, Boon Kee
Koh, Soo Peng
Long, Kamariah
Alitheen, Noorjahan Banu
author_sort Yusof, Hamidah Mohd
collection PubMed
description BACKGROUND: Tempeh is a widely known fermented soybean that contains elevated level of bioactive contents. Our previous study has shown that anaerobic fermented Nutrient Enriched Soybean Tempeh (NESTE) with increase amino acid and antioxidant levels possessed better hepatoprotective effect than raw soybean. METHODS: In this study, the anti-inflammatory effect of the NESTE aqueous extract and raw soybean aqueous extract (SBE) were evaluated by quantifying the inhibition of IL-1β, TNF-α and nitric oxide (NO) secretion in LPS treated RAW 264.7 cell in vitro. On the other hand, in vivo oral acute toxicity effect of the extract was tested on mice at the dose of 5000 mg/kg body weight. In vivo oral analgesic effect of both aqueous extracts at 200 and 1000 mg/kg body weight was evaluated by the hot plate test. RESULTS: In the in vitro anti-inflammatory study, 5 mg/mL NESTE was able to inhibit 25.50 ± 2.20%, 35.88 ± 3.20% and 28.50 ± 3.50% of NO, IL-1β and TNF-α production in LPS treated RAW 264.7 cells without inducing cytotoxic effect on the cells. However, this effect was lower than 4 μg/mL of curcumin, which inhibited NO, IL-1β and TNF-α production by 89.50 ± 5.00%, 78.80 ± 6.20% and 87.30 ± 4.00%, respectively. In addition, 1.5 to 2.5-fold increase of latency period up to 120 min for mice in the hot plate test was achieved by 1000 mg/kg NESTE. The analgesic effect of NESTE was better than 400 mg/kg of acetyl salicylic acid, which only increased ~ 1.7-fold of latency period up to 90 min. Moreover, NESTE did not show acute toxicity (no LD(50)) up to 5000 mg/kg body weight. CONCLUSION: NESTE is a nutritious food ingredient with potential anti-inflammatory and analgesic effects.
format Online
Article
Text
id pubmed-6923968
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-69239682019-12-30 Anti-inflammatory, analgesic and acute toxicity effects of fermented soybean Yusof, Hamidah Mohd Ali, Norlaily Mohd Yeap, Swee Keong Ho, Wan Yong Beh, Boon Kee Koh, Soo Peng Long, Kamariah Alitheen, Noorjahan Banu BMC Complement Altern Med Research Article BACKGROUND: Tempeh is a widely known fermented soybean that contains elevated level of bioactive contents. Our previous study has shown that anaerobic fermented Nutrient Enriched Soybean Tempeh (NESTE) with increase amino acid and antioxidant levels possessed better hepatoprotective effect than raw soybean. METHODS: In this study, the anti-inflammatory effect of the NESTE aqueous extract and raw soybean aqueous extract (SBE) were evaluated by quantifying the inhibition of IL-1β, TNF-α and nitric oxide (NO) secretion in LPS treated RAW 264.7 cell in vitro. On the other hand, in vivo oral acute toxicity effect of the extract was tested on mice at the dose of 5000 mg/kg body weight. In vivo oral analgesic effect of both aqueous extracts at 200 and 1000 mg/kg body weight was evaluated by the hot plate test. RESULTS: In the in vitro anti-inflammatory study, 5 mg/mL NESTE was able to inhibit 25.50 ± 2.20%, 35.88 ± 3.20% and 28.50 ± 3.50% of NO, IL-1β and TNF-α production in LPS treated RAW 264.7 cells without inducing cytotoxic effect on the cells. However, this effect was lower than 4 μg/mL of curcumin, which inhibited NO, IL-1β and TNF-α production by 89.50 ± 5.00%, 78.80 ± 6.20% and 87.30 ± 4.00%, respectively. In addition, 1.5 to 2.5-fold increase of latency period up to 120 min for mice in the hot plate test was achieved by 1000 mg/kg NESTE. The analgesic effect of NESTE was better than 400 mg/kg of acetyl salicylic acid, which only increased ~ 1.7-fold of latency period up to 90 min. Moreover, NESTE did not show acute toxicity (no LD(50)) up to 5000 mg/kg body weight. CONCLUSION: NESTE is a nutritious food ingredient with potential anti-inflammatory and analgesic effects. BioMed Central 2019-12-19 /pmc/articles/PMC6923968/ /pubmed/31856816 http://dx.doi.org/10.1186/s12906-019-2791-2 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Yusof, Hamidah Mohd
Ali, Norlaily Mohd
Yeap, Swee Keong
Ho, Wan Yong
Beh, Boon Kee
Koh, Soo Peng
Long, Kamariah
Alitheen, Noorjahan Banu
Anti-inflammatory, analgesic and acute toxicity effects of fermented soybean
title Anti-inflammatory, analgesic and acute toxicity effects of fermented soybean
title_full Anti-inflammatory, analgesic and acute toxicity effects of fermented soybean
title_fullStr Anti-inflammatory, analgesic and acute toxicity effects of fermented soybean
title_full_unstemmed Anti-inflammatory, analgesic and acute toxicity effects of fermented soybean
title_short Anti-inflammatory, analgesic and acute toxicity effects of fermented soybean
title_sort anti-inflammatory, analgesic and acute toxicity effects of fermented soybean
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923968/
https://www.ncbi.nlm.nih.gov/pubmed/31856816
http://dx.doi.org/10.1186/s12906-019-2791-2
work_keys_str_mv AT yusofhamidahmohd antiinflammatoryanalgesicandacutetoxicityeffectsoffermentedsoybean
AT alinorlailymohd antiinflammatoryanalgesicandacutetoxicityeffectsoffermentedsoybean
AT yeapsweekeong antiinflammatoryanalgesicandacutetoxicityeffectsoffermentedsoybean
AT howanyong antiinflammatoryanalgesicandacutetoxicityeffectsoffermentedsoybean
AT behboonkee antiinflammatoryanalgesicandacutetoxicityeffectsoffermentedsoybean
AT kohsoopeng antiinflammatoryanalgesicandacutetoxicityeffectsoffermentedsoybean
AT longkamariah antiinflammatoryanalgesicandacutetoxicityeffectsoffermentedsoybean
AT alitheennoorjahanbanu antiinflammatoryanalgesicandacutetoxicityeffectsoffermentedsoybean