Assessment of differences between DNA content of cell-cultured and freely suspended oocysts of Cryptosporidium parvum and their suitability as DNA standards in qPCR

BACKGROUND: Although more modern methods are available, quantitative PCR (qPCR) is reproducible, sensitive and specific with instruments and expertise readily available in many laboratories. As such, the use of qPCR in Cryptosporidium research is well established and still widely used by researchers...

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Detalles Bibliográficos
Autores principales: Woolsey, Ian D., Blomstrand, Berit, Øines, Øivind, Enemark, Heidi L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Methodology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6923971/
https://www.ncbi.nlm.nih.gov/pubmed/31856894
http://dx.doi.org/10.1186/s13071-019-3851-7

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