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Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface

Intestinal epithelium functions as a barrier to protect multicellular organisms from the outside world. It consists of epithelial cells closely connected by intercellular junctions, selective gates which control paracellular diffusion of solutes, ions and macromolecules across the epithelium and kee...

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Autores principales: Cui, Tingting, Theuns, Sebastiaan, Xie, Jiexiong, Nauwynck, Hans J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6924034/
https://www.ncbi.nlm.nih.gov/pubmed/31856906
http://dx.doi.org/10.1186/s13567-019-0728-x
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author Cui, Tingting
Theuns, Sebastiaan
Xie, Jiexiong
Nauwynck, Hans J.
author_facet Cui, Tingting
Theuns, Sebastiaan
Xie, Jiexiong
Nauwynck, Hans J.
author_sort Cui, Tingting
collection PubMed
description Intestinal epithelium functions as a barrier to protect multicellular organisms from the outside world. It consists of epithelial cells closely connected by intercellular junctions, selective gates which control paracellular diffusion of solutes, ions and macromolecules across the epithelium and keep out pathogens. Rotavirus is one of the major enteric viruses causing severe diarrhea in humans and animals. It specifically infects the enterocytes on villi of small intestines. The polarity of rotavirus replication in their target enterocytes and the role of intestinal epithelial integrity were examined in the present study. Treatment with EGTA, a drug that chelates calcium and disrupts the intercellular junctions, (i) significantly enhanced the infection of rotavirus in primary enterocytes, (ii) increased the binding of rotavirus to enterocytes, but (iii) considerably blocked internalization of rotavirus. After internalization, rotavirus was resistant to EGTA treatment. To investigate the polarity of rotavirus infection, the primary enterocytes were cultured in a transwell system and infected with rotavirus at either the apical or the basolateral surface. Rotavirus preferentially infected enterocytes at the basolateral surface. Restriction of infection through apical inoculation was overcome by EGTA treatment. Overall, our findings demonstrate that integrity of the intestinal epithelium is crucial in the host’s innate defense against rotavirus infection. In addition, the intercellular receptor is located basolaterally and disruption of intercellular junctions facilitates the binding of rotavirus to their receptor at the basolateral surface.
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spelling pubmed-69240342019-12-30 Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface Cui, Tingting Theuns, Sebastiaan Xie, Jiexiong Nauwynck, Hans J. Vet Res Research Article Intestinal epithelium functions as a barrier to protect multicellular organisms from the outside world. It consists of epithelial cells closely connected by intercellular junctions, selective gates which control paracellular diffusion of solutes, ions and macromolecules across the epithelium and keep out pathogens. Rotavirus is one of the major enteric viruses causing severe diarrhea in humans and animals. It specifically infects the enterocytes on villi of small intestines. The polarity of rotavirus replication in their target enterocytes and the role of intestinal epithelial integrity were examined in the present study. Treatment with EGTA, a drug that chelates calcium and disrupts the intercellular junctions, (i) significantly enhanced the infection of rotavirus in primary enterocytes, (ii) increased the binding of rotavirus to enterocytes, but (iii) considerably blocked internalization of rotavirus. After internalization, rotavirus was resistant to EGTA treatment. To investigate the polarity of rotavirus infection, the primary enterocytes were cultured in a transwell system and infected with rotavirus at either the apical or the basolateral surface. Rotavirus preferentially infected enterocytes at the basolateral surface. Restriction of infection through apical inoculation was overcome by EGTA treatment. Overall, our findings demonstrate that integrity of the intestinal epithelium is crucial in the host’s innate defense against rotavirus infection. In addition, the intercellular receptor is located basolaterally and disruption of intercellular junctions facilitates the binding of rotavirus to their receptor at the basolateral surface. BioMed Central 2019-12-19 2019 /pmc/articles/PMC6924034/ /pubmed/31856906 http://dx.doi.org/10.1186/s13567-019-0728-x Text en © The Author(s) 2019 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Cui, Tingting
Theuns, Sebastiaan
Xie, Jiexiong
Nauwynck, Hans J.
Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface
title Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface
title_full Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface
title_fullStr Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface
title_full_unstemmed Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface
title_short Porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface
title_sort porcine rotavirus mainly infects primary porcine enterocytes at the basolateral surface
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6924034/
https://www.ncbi.nlm.nih.gov/pubmed/31856906
http://dx.doi.org/10.1186/s13567-019-0728-x
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