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miRNA-520c-3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A

Biological function of microRNA-20c-3p (miRNA-520c-3p) in the progression of nasopharyngeal carcinoma (NPC) and the potential mechanism were investigated. Relative level of miRNA-520c-3p in NPC tissues and adjacent normal tissues was determined by quantitative real-time polymerase chain reaction (qR...

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Autores principales: Sun, Xiaohan, Xu, Wenrui, Zang, Chuanshan, Li, Na
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6924133/
https://www.ncbi.nlm.nih.gov/pubmed/31897193
http://dx.doi.org/10.3892/ol.2019.11144
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author Sun, Xiaohan
Xu, Wenrui
Zang, Chuanshan
Li, Na
author_facet Sun, Xiaohan
Xu, Wenrui
Zang, Chuanshan
Li, Na
author_sort Sun, Xiaohan
collection PubMed
description Biological function of microRNA-20c-3p (miRNA-520c-3p) in the progression of nasopharyngeal carcinoma (NPC) and the potential mechanism were investigated. Relative level of miRNA-520c-3p in NPC tissues and adjacent normal tissues was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Particularly, miRNA-520c-3p level in NPC with different tumor stages and tumor sizes was examined. Subsequently, miRNA-520c-3p level in nasopharyngeal epithelial cells and NPC cells was detected. The potential influence of miRNA-520c-3p on the proliferative ability and cell cycle progression of NPC cells were evaluated through cell counting kit-8 (CCK-8) and flow cytometry. The target gene of miRNA-520c-3p was verified by dual-luciferase reporter gene assay. Regulatory role of miRNA-520c-3p/RAB22A in the malignant progression of NPC was identified. miRNA-520c-3p was downregulated in NPC tissues and cell lines. Its level was lower in NPC with worse tumor grade and larger tumor size. Overexpression of miRNA-520c-3p suppressed the proliferative ability and arrested cell cycle in G0/G1 phase. RAB22A was confirmed to be the downstream target of miRNA-520c-3p. In NPC tissues and cell lines, RAB22A remained in higher abundance relative to controls. Overexpression of RAB22A reversed the inhibitory effects of overexpressed miRNA-520c-3p on proliferative ability and cell cycle progression of NPC cells. miRNA-520c-3p is downregulated in NPC, which accelerates the malignant progression of NPC by targeting RAB22A.
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spelling pubmed-69241332020-01-02 miRNA-520c-3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A Sun, Xiaohan Xu, Wenrui Zang, Chuanshan Li, Na Oncol Lett Articles Biological function of microRNA-20c-3p (miRNA-520c-3p) in the progression of nasopharyngeal carcinoma (NPC) and the potential mechanism were investigated. Relative level of miRNA-520c-3p in NPC tissues and adjacent normal tissues was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Particularly, miRNA-520c-3p level in NPC with different tumor stages and tumor sizes was examined. Subsequently, miRNA-520c-3p level in nasopharyngeal epithelial cells and NPC cells was detected. The potential influence of miRNA-520c-3p on the proliferative ability and cell cycle progression of NPC cells were evaluated through cell counting kit-8 (CCK-8) and flow cytometry. The target gene of miRNA-520c-3p was verified by dual-luciferase reporter gene assay. Regulatory role of miRNA-520c-3p/RAB22A in the malignant progression of NPC was identified. miRNA-520c-3p was downregulated in NPC tissues and cell lines. Its level was lower in NPC with worse tumor grade and larger tumor size. Overexpression of miRNA-520c-3p suppressed the proliferative ability and arrested cell cycle in G0/G1 phase. RAB22A was confirmed to be the downstream target of miRNA-520c-3p. In NPC tissues and cell lines, RAB22A remained in higher abundance relative to controls. Overexpression of RAB22A reversed the inhibitory effects of overexpressed miRNA-520c-3p on proliferative ability and cell cycle progression of NPC cells. miRNA-520c-3p is downregulated in NPC, which accelerates the malignant progression of NPC by targeting RAB22A. D.A. Spandidos 2020-01 2019-11-25 /pmc/articles/PMC6924133/ /pubmed/31897193 http://dx.doi.org/10.3892/ol.2019.11144 Text en Copyright: © Sun et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Sun, Xiaohan
Xu, Wenrui
Zang, Chuanshan
Li, Na
miRNA-520c-3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A
title miRNA-520c-3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A
title_full miRNA-520c-3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A
title_fullStr miRNA-520c-3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A
title_full_unstemmed miRNA-520c-3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A
title_short miRNA-520c-3p accelerates progression of nasopharyngeal carcinoma via targeting RAB22A
title_sort mirna-520c-3p accelerates progression of nasopharyngeal carcinoma via targeting rab22a
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6924133/
https://www.ncbi.nlm.nih.gov/pubmed/31897193
http://dx.doi.org/10.3892/ol.2019.11144
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