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Identification and chemoresistance of cancer stem cells in HPV-negative oropharyngeal cancer

The underlying mechanisms of resistance to chemoradiotherapy of human papilloma virus (HPV)-negative patients with oropharyngeal cancer (OPC) remain unclear. The present study aimed to characterize cancer stem cells (CSC) of the HPV-negative OPC cell line in terms of chemotherapy resistance. CSCs we...

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Autores principales: Gunduz, Mehmet, Gunduz, Esra, Tamagawa, Shunji, Enomoto, Keisuke, Hotomi, Muneki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6924148/
https://www.ncbi.nlm.nih.gov/pubmed/31897209
http://dx.doi.org/10.3892/ol.2019.11127
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author Gunduz, Mehmet
Gunduz, Esra
Tamagawa, Shunji
Enomoto, Keisuke
Hotomi, Muneki
author_facet Gunduz, Mehmet
Gunduz, Esra
Tamagawa, Shunji
Enomoto, Keisuke
Hotomi, Muneki
author_sort Gunduz, Mehmet
collection PubMed
description The underlying mechanisms of resistance to chemoradiotherapy of human papilloma virus (HPV)-negative patients with oropharyngeal cancer (OPC) remain unclear. The present study aimed to characterize cancer stem cells (CSC) of the HPV-negative OPC cell line in terms of chemotherapy resistance. CSCs were isolated through magnetic activated cell sorting using the CSC specific marker aldehyde dehydrogenase 1 antibody, and characterized by sphere formation capacity, immunofluorescence staining, and CSC marker expression. CSC response to cisplatin treatment was evaluated via XTT-assays. Spheres of CSCs of the HPV-negative UTSCC-60A cell line were highly dark holospheres. RNA expression levels of CSC markers OCT4, SOX2, Kruppel-like factor 4 and BMI1 were significantly higher in CSC. CSCs were significantly resistant to cisplatin treatment at various dosages compared with nonCSC. The present study suggested that the proportion of CSCs is very low in the tumor bulk, CSCs are resistant to cisplatin in HPV-negative OPC, which requires further investigation to define their mechanism.
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spelling pubmed-69241482020-01-02 Identification and chemoresistance of cancer stem cells in HPV-negative oropharyngeal cancer Gunduz, Mehmet Gunduz, Esra Tamagawa, Shunji Enomoto, Keisuke Hotomi, Muneki Oncol Lett Articles The underlying mechanisms of resistance to chemoradiotherapy of human papilloma virus (HPV)-negative patients with oropharyngeal cancer (OPC) remain unclear. The present study aimed to characterize cancer stem cells (CSC) of the HPV-negative OPC cell line in terms of chemotherapy resistance. CSCs were isolated through magnetic activated cell sorting using the CSC specific marker aldehyde dehydrogenase 1 antibody, and characterized by sphere formation capacity, immunofluorescence staining, and CSC marker expression. CSC response to cisplatin treatment was evaluated via XTT-assays. Spheres of CSCs of the HPV-negative UTSCC-60A cell line were highly dark holospheres. RNA expression levels of CSC markers OCT4, SOX2, Kruppel-like factor 4 and BMI1 were significantly higher in CSC. CSCs were significantly resistant to cisplatin treatment at various dosages compared with nonCSC. The present study suggested that the proportion of CSCs is very low in the tumor bulk, CSCs are resistant to cisplatin in HPV-negative OPC, which requires further investigation to define their mechanism. D.A. Spandidos 2020-01 2019-11-21 /pmc/articles/PMC6924148/ /pubmed/31897209 http://dx.doi.org/10.3892/ol.2019.11127 Text en Copyright: © Gunduz et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Gunduz, Mehmet
Gunduz, Esra
Tamagawa, Shunji
Enomoto, Keisuke
Hotomi, Muneki
Identification and chemoresistance of cancer stem cells in HPV-negative oropharyngeal cancer
title Identification and chemoresistance of cancer stem cells in HPV-negative oropharyngeal cancer
title_full Identification and chemoresistance of cancer stem cells in HPV-negative oropharyngeal cancer
title_fullStr Identification and chemoresistance of cancer stem cells in HPV-negative oropharyngeal cancer
title_full_unstemmed Identification and chemoresistance of cancer stem cells in HPV-negative oropharyngeal cancer
title_short Identification and chemoresistance of cancer stem cells in HPV-negative oropharyngeal cancer
title_sort identification and chemoresistance of cancer stem cells in hpv-negative oropharyngeal cancer
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6924148/
https://www.ncbi.nlm.nih.gov/pubmed/31897209
http://dx.doi.org/10.3892/ol.2019.11127
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