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Relationship between certain serum biochemical values and serostatus against Anaplasma marginale in dairy cows
AIM: This study was conducted to evaluate the possible association between values of certain serum biochemical parameters and seropositivity against Anaplasma marginale in dairy cows. MATERIALS AND METHODS: Serum samples from 60 seropositive and 40 seronegative cows were used to determine the values...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Veterinary World
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925038/ https://www.ncbi.nlm.nih.gov/pubmed/32009766 http://dx.doi.org/10.14202/vetworld.2019.1858-1861 |
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author | Alekish, Myassar O. Ismail, Zuhair Bani |
author_facet | Alekish, Myassar O. Ismail, Zuhair Bani |
author_sort | Alekish, Myassar O. |
collection | PubMed |
description | AIM: This study was conducted to evaluate the possible association between values of certain serum biochemical parameters and seropositivity against Anaplasma marginale in dairy cows. MATERIALS AND METHODS: Serum samples from 60 seropositive and 40 seronegative cows were used to determine the values of beta-hydroxybutyrate (BHB), glucose, creatinine, blood urea nitrogen, total protein, albumin, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), and gamma-glutamyl transferase (GGT) using commercially available kits and reagents. The serostatus of cows against A. marginale was determined using a commercially available cellular enzyme-linked immunosorbent assay kit according to the manufacturer’s recommendations. Significant differences in serum biochemical values between seropositive and seronegative groups were evaluated using independent Student’s t-test. Possible associations between the serostatus of the cows and different biochemical parameters were evaluated using univariate followed by multivariate logistic regression analyses. RESULTS: There was a statistically significant increase (p≤0.05) in values of total protein, BHB, LDH, and AST in seropositive cows compared to seronegative cows while a non-significant increase in values of ALP, ALT, and GGT was detected in seropositive cows. A strong correlation (R=0.69) between serum levels of BHB, LDH, and AST and seropositivity against A. marginale was detected. CONCLUSION: There is evidence of a possible association between A. marginale infection and liver damage/hepatic fatty degeneration in dairy cows. Further studies, however, are required to elucidate the exact pathophysiological mechanisms of this relationship. |
format | Online Article Text |
id | pubmed-6925038 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Veterinary World |
record_format | MEDLINE/PubMed |
spelling | pubmed-69250382020-01-31 Relationship between certain serum biochemical values and serostatus against Anaplasma marginale in dairy cows Alekish, Myassar O. Ismail, Zuhair Bani Vet World Research Article AIM: This study was conducted to evaluate the possible association between values of certain serum biochemical parameters and seropositivity against Anaplasma marginale in dairy cows. MATERIALS AND METHODS: Serum samples from 60 seropositive and 40 seronegative cows were used to determine the values of beta-hydroxybutyrate (BHB), glucose, creatinine, blood urea nitrogen, total protein, albumin, alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH), and gamma-glutamyl transferase (GGT) using commercially available kits and reagents. The serostatus of cows against A. marginale was determined using a commercially available cellular enzyme-linked immunosorbent assay kit according to the manufacturer’s recommendations. Significant differences in serum biochemical values between seropositive and seronegative groups were evaluated using independent Student’s t-test. Possible associations between the serostatus of the cows and different biochemical parameters were evaluated using univariate followed by multivariate logistic regression analyses. RESULTS: There was a statistically significant increase (p≤0.05) in values of total protein, BHB, LDH, and AST in seropositive cows compared to seronegative cows while a non-significant increase in values of ALP, ALT, and GGT was detected in seropositive cows. A strong correlation (R=0.69) between serum levels of BHB, LDH, and AST and seropositivity against A. marginale was detected. CONCLUSION: There is evidence of a possible association between A. marginale infection and liver damage/hepatic fatty degeneration in dairy cows. Further studies, however, are required to elucidate the exact pathophysiological mechanisms of this relationship. Veterinary World 2019-11 2019-11-26 /pmc/articles/PMC6925038/ /pubmed/32009766 http://dx.doi.org/10.14202/vetworld.2019.1858-1861 Text en Copyright: © Alekish and Ismail. http://creativecommons.org/licenses/by/4.0 Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Alekish, Myassar O. Ismail, Zuhair Bani Relationship between certain serum biochemical values and serostatus against Anaplasma marginale in dairy cows |
title | Relationship between certain serum biochemical values and serostatus against Anaplasma marginale in dairy cows |
title_full | Relationship between certain serum biochemical values and serostatus against Anaplasma marginale in dairy cows |
title_fullStr | Relationship between certain serum biochemical values and serostatus against Anaplasma marginale in dairy cows |
title_full_unstemmed | Relationship between certain serum biochemical values and serostatus against Anaplasma marginale in dairy cows |
title_short | Relationship between certain serum biochemical values and serostatus against Anaplasma marginale in dairy cows |
title_sort | relationship between certain serum biochemical values and serostatus against anaplasma marginale in dairy cows |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925038/ https://www.ncbi.nlm.nih.gov/pubmed/32009766 http://dx.doi.org/10.14202/vetworld.2019.1858-1861 |
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