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Biosynthetic approach to combine the first steps of cardenolide formation in Saccharomyces cerevisiae

A yeast expression plasmid was constructed containing a cardenolide biosynthetic module, referred to as CARD II, using the AssemblX toolkit, which enables the assembly of large DNA constructs. The genes cloned into the vector were (a) a Δ(5)‐3β‐hydroxysteroid dehydrogenase gene from Digitalis lanata...

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Detalles Bibliográficos
Autores principales:	Rieck, Christoph, Geiger, Daniel, Munkert, Jennifer, Messerschmidt, Katrin, Petersen, Jan, Strasser, Juliane, Meitinger, Nadine, Kreis, Wolfgang
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2019
Materias:	Original Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925150/ https://www.ncbi.nlm.nih.gov/pubmed/31436030 http://dx.doi.org/10.1002/mbo3.925

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