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Heat‐induced shift in coral microbiome reveals several members of the Rhodobacteraceae family as indicator species for thermal stress in Porites lutea

The coral holobiont is a complex ecosystem consisting of coral animals and a highly diverse consortium of associated microorganisms including algae, fungi, and bacteria. Several studies have highlighted the importance of coral‐associated bacteria and their potential roles in promoting the host fitne...

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Autores principales: Pootakham, Wirulda, Mhuantong, Wuttichai, Yoocha, Thippawan, Putchim, Lalita, Jomchai, Nukoon, Sonthirod, Chutima, Naktang, Chaiwat, Kongkachana, Wasitthee, Tangphatsornruang, Sithichoke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925168/
https://www.ncbi.nlm.nih.gov/pubmed/31544365
http://dx.doi.org/10.1002/mbo3.935
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author Pootakham, Wirulda
Mhuantong, Wuttichai
Yoocha, Thippawan
Putchim, Lalita
Jomchai, Nukoon
Sonthirod, Chutima
Naktang, Chaiwat
Kongkachana, Wasitthee
Tangphatsornruang, Sithichoke
author_facet Pootakham, Wirulda
Mhuantong, Wuttichai
Yoocha, Thippawan
Putchim, Lalita
Jomchai, Nukoon
Sonthirod, Chutima
Naktang, Chaiwat
Kongkachana, Wasitthee
Tangphatsornruang, Sithichoke
author_sort Pootakham, Wirulda
collection PubMed
description The coral holobiont is a complex ecosystem consisting of coral animals and a highly diverse consortium of associated microorganisms including algae, fungi, and bacteria. Several studies have highlighted the importance of coral‐associated bacteria and their potential roles in promoting the host fitness and survival. Recently, dynamics of coral‐associated microbiomes have been demonstrated to be linked to patterns of coral heat tolerance. Here, we examined the effect of elevated seawater temperature on the structure and diversity of bacterial populations associated with Porites lutea, using full‐length 16S rRNA sequences obtained from Pacific Biosciences circular consensus sequencing. We observed a significant increase in alpha diversity indices and a distinct shift in microbiome composition during thermal stress. There was a marked decline in the apparent relative abundance of Gammaproteobacteria family Endozoicomonadaceae after P. lutea had been exposed to elevated seawater temperature. Concomitantly, the bacterial community structure shifted toward the predominance of Alphaproteobacteria family Rhodobacteraceae. Interestingly, we did not observe an increase in relative abundance of Vibrio‐related sequences in our heat‐stressed samples even though the appearance of Vibrio spp. has often been detected in parallel with the increase in the relative abundance of Rhodobacteraceae during thermal bleaching in other coral species. The ability of full‐length 16S rRNA sequences in resolving taxonomic uncertainty of associated bacteria at a species level enabled us to identify 24 robust indicator bacterial species for thermally stressed corals. It is worth noting that the majority of those indicator species were members of the family Rhodobacteraceae. The comparison of bacterial community structure and diversity between corals in ambient water temperature and thermally stressed corals may provide a better understanding on how bacteria symbionts contribute to the resilience of their coral hosts to ocean warming.
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spelling pubmed-69251682019-12-24 Heat‐induced shift in coral microbiome reveals several members of the Rhodobacteraceae family as indicator species for thermal stress in Porites lutea Pootakham, Wirulda Mhuantong, Wuttichai Yoocha, Thippawan Putchim, Lalita Jomchai, Nukoon Sonthirod, Chutima Naktang, Chaiwat Kongkachana, Wasitthee Tangphatsornruang, Sithichoke Microbiologyopen Original Articles The coral holobiont is a complex ecosystem consisting of coral animals and a highly diverse consortium of associated microorganisms including algae, fungi, and bacteria. Several studies have highlighted the importance of coral‐associated bacteria and their potential roles in promoting the host fitness and survival. Recently, dynamics of coral‐associated microbiomes have been demonstrated to be linked to patterns of coral heat tolerance. Here, we examined the effect of elevated seawater temperature on the structure and diversity of bacterial populations associated with Porites lutea, using full‐length 16S rRNA sequences obtained from Pacific Biosciences circular consensus sequencing. We observed a significant increase in alpha diversity indices and a distinct shift in microbiome composition during thermal stress. There was a marked decline in the apparent relative abundance of Gammaproteobacteria family Endozoicomonadaceae after P. lutea had been exposed to elevated seawater temperature. Concomitantly, the bacterial community structure shifted toward the predominance of Alphaproteobacteria family Rhodobacteraceae. Interestingly, we did not observe an increase in relative abundance of Vibrio‐related sequences in our heat‐stressed samples even though the appearance of Vibrio spp. has often been detected in parallel with the increase in the relative abundance of Rhodobacteraceae during thermal bleaching in other coral species. The ability of full‐length 16S rRNA sequences in resolving taxonomic uncertainty of associated bacteria at a species level enabled us to identify 24 robust indicator bacterial species for thermally stressed corals. It is worth noting that the majority of those indicator species were members of the family Rhodobacteraceae. The comparison of bacterial community structure and diversity between corals in ambient water temperature and thermally stressed corals may provide a better understanding on how bacteria symbionts contribute to the resilience of their coral hosts to ocean warming. John Wiley and Sons Inc. 2019-09-23 /pmc/articles/PMC6925168/ /pubmed/31544365 http://dx.doi.org/10.1002/mbo3.935 Text en © 2019 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd. This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Original Articles
Pootakham, Wirulda
Mhuantong, Wuttichai
Yoocha, Thippawan
Putchim, Lalita
Jomchai, Nukoon
Sonthirod, Chutima
Naktang, Chaiwat
Kongkachana, Wasitthee
Tangphatsornruang, Sithichoke
Heat‐induced shift in coral microbiome reveals several members of the Rhodobacteraceae family as indicator species for thermal stress in Porites lutea
title Heat‐induced shift in coral microbiome reveals several members of the Rhodobacteraceae family as indicator species for thermal stress in Porites lutea
title_full Heat‐induced shift in coral microbiome reveals several members of the Rhodobacteraceae family as indicator species for thermal stress in Porites lutea
title_fullStr Heat‐induced shift in coral microbiome reveals several members of the Rhodobacteraceae family as indicator species for thermal stress in Porites lutea
title_full_unstemmed Heat‐induced shift in coral microbiome reveals several members of the Rhodobacteraceae family as indicator species for thermal stress in Porites lutea
title_short Heat‐induced shift in coral microbiome reveals several members of the Rhodobacteraceae family as indicator species for thermal stress in Porites lutea
title_sort heat‐induced shift in coral microbiome reveals several members of the rhodobacteraceae family as indicator species for thermal stress in porites lutea
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925168/
https://www.ncbi.nlm.nih.gov/pubmed/31544365
http://dx.doi.org/10.1002/mbo3.935
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