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Downregulation of lncRNA NCK1-AS1 Inhibits Cancer Cell Migration and Invasion in Nasopharyngeal Carcinoma by Upregulating miR-135a

INTRODUCTION: The present study was carried out to explore the functionality of lncRNA NCK1-AS1 in nasopharyngeal carcinoma (NPC). METHODS: Levels of NCK1-AS1 were measured by performing qPCR and were compared by ANOVA (one-way) performed in combination with Tukey’s test. Expression levels of miR-13...

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Autores principales: Hu, Haili, Li, Haixia, Feng, Xiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925550/
https://www.ncbi.nlm.nih.gov/pubmed/31908525
http://dx.doi.org/10.2147/CMAR.S221326
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author Hu, Haili
Li, Haixia
Feng, Xiao
author_facet Hu, Haili
Li, Haixia
Feng, Xiao
author_sort Hu, Haili
collection PubMed
description INTRODUCTION: The present study was carried out to explore the functionality of lncRNA NCK1-AS1 in nasopharyngeal carcinoma (NPC). METHODS: Levels of NCK1-AS1 were measured by performing qPCR and were compared by ANOVA (one-way) performed in combination with Tukey’s test. Expression levels of miR-135a in plasma of NPC patients were measured by performing qPCR. The effects of transfections on the invasion and migration of C666-1 cells were analyzed by Transwell assays. RESULTS AND DISCUSSION: In the present study, we found that the plasma levels of NCK1-AS1 were significantly higher in NPC patients than the levels in patients with arthritis of the temporomandibular joint (TMJ), as well as healthy participants. No significant difference in plasma levels of NCK1-AS1 was found between TMJ patients and healthy participants. Upregulation of NCK1-AS1 distinguished NPC patients from TMJ patients and healthy participants. A significant and inverse correlation between NCK1-AS1 and miR-135a was found in NPC patients. NCK1-AS1 siRNA silencing led to the upregulation of miR-135a. NCK1-AS1 siRNA silencing and miR-135a overexpression resulted in inhibited cell migration and invasion, and miR-135a inhibition attenuated the effects of NCK1-AS1 siRNA silencing. CONCLUSION: The downregulation of lncRNA NCK1-AS1 inhibited cancer cell migration and invasion in NPC by upregulating miR-135a.
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spelling pubmed-69255502020-01-06 Downregulation of lncRNA NCK1-AS1 Inhibits Cancer Cell Migration and Invasion in Nasopharyngeal Carcinoma by Upregulating miR-135a Hu, Haili Li, Haixia Feng, Xiao Cancer Manag Res Original Research INTRODUCTION: The present study was carried out to explore the functionality of lncRNA NCK1-AS1 in nasopharyngeal carcinoma (NPC). METHODS: Levels of NCK1-AS1 were measured by performing qPCR and were compared by ANOVA (one-way) performed in combination with Tukey’s test. Expression levels of miR-135a in plasma of NPC patients were measured by performing qPCR. The effects of transfections on the invasion and migration of C666-1 cells were analyzed by Transwell assays. RESULTS AND DISCUSSION: In the present study, we found that the plasma levels of NCK1-AS1 were significantly higher in NPC patients than the levels in patients with arthritis of the temporomandibular joint (TMJ), as well as healthy participants. No significant difference in plasma levels of NCK1-AS1 was found between TMJ patients and healthy participants. Upregulation of NCK1-AS1 distinguished NPC patients from TMJ patients and healthy participants. A significant and inverse correlation between NCK1-AS1 and miR-135a was found in NPC patients. NCK1-AS1 siRNA silencing led to the upregulation of miR-135a. NCK1-AS1 siRNA silencing and miR-135a overexpression resulted in inhibited cell migration and invasion, and miR-135a inhibition attenuated the effects of NCK1-AS1 siRNA silencing. CONCLUSION: The downregulation of lncRNA NCK1-AS1 inhibited cancer cell migration and invasion in NPC by upregulating miR-135a. Dove 2019-12-17 /pmc/articles/PMC6925550/ /pubmed/31908525 http://dx.doi.org/10.2147/CMAR.S221326 Text en © 2019 Hu et al. http://creativecommons.org/licenses/by-nc/3.0/ This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Hu, Haili
Li, Haixia
Feng, Xiao
Downregulation of lncRNA NCK1-AS1 Inhibits Cancer Cell Migration and Invasion in Nasopharyngeal Carcinoma by Upregulating miR-135a
title Downregulation of lncRNA NCK1-AS1 Inhibits Cancer Cell Migration and Invasion in Nasopharyngeal Carcinoma by Upregulating miR-135a
title_full Downregulation of lncRNA NCK1-AS1 Inhibits Cancer Cell Migration and Invasion in Nasopharyngeal Carcinoma by Upregulating miR-135a
title_fullStr Downregulation of lncRNA NCK1-AS1 Inhibits Cancer Cell Migration and Invasion in Nasopharyngeal Carcinoma by Upregulating miR-135a
title_full_unstemmed Downregulation of lncRNA NCK1-AS1 Inhibits Cancer Cell Migration and Invasion in Nasopharyngeal Carcinoma by Upregulating miR-135a
title_short Downregulation of lncRNA NCK1-AS1 Inhibits Cancer Cell Migration and Invasion in Nasopharyngeal Carcinoma by Upregulating miR-135a
title_sort downregulation of lncrna nck1-as1 inhibits cancer cell migration and invasion in nasopharyngeal carcinoma by upregulating mir-135a
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6925550/
https://www.ncbi.nlm.nih.gov/pubmed/31908525
http://dx.doi.org/10.2147/CMAR.S221326
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