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Amplification of Stem Genes: New Potential Metastatic Makers in Patients with an Early Form of Breast Cancer

BACKGROUND: According to our previous studies, the presence of amplifications of stem genes can lead to their ectopic expression and this is associated with an increased activity of tumor stem cells in these patients. This leads to a high aggressiveness of the tumor and the development of metastatic...

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Autores principales: Tsyganov, Matvey M., Ibragimova, Marina K., Pevzner, Alina M., Doroshenko, Artem V., Slonimskaya, Elena M., Litviakov, Nikolai V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Academy of Medical Sciences 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6926101/
https://www.ncbi.nlm.nih.gov/pubmed/31858753
http://dx.doi.org/10.3346/jkms.2019.34.e312
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author Tsyganov, Matvey M.
Ibragimova, Marina K.
Pevzner, Alina M.
Doroshenko, Artem V.
Slonimskaya, Elena M.
Litviakov, Nikolai V.
author_facet Tsyganov, Matvey M.
Ibragimova, Marina K.
Pevzner, Alina M.
Doroshenko, Artem V.
Slonimskaya, Elena M.
Litviakov, Nikolai V.
author_sort Tsyganov, Matvey M.
collection PubMed
description BACKGROUND: According to our previous studies, the presence of amplifications of stem genes can lead to their ectopic expression and this is associated with an increased activity of tumor stem cells in these patients. This leads to a high aggressiveness of the tumor and the development of metastatic disease. The aim was to evaluate the prognostic significance of the presence of amplifications of stem genes and their expression in patients with early breast cancer (BC). METHODS: The study included 28 patients with T(1)N(x)M(0) BC. We used surgical specimens, including formalin-fixed paraffin-embedded archive materials, for 8 patients. A microarray analysis was performed on high-density DNA chips from CytoScanHDArray to assess the status of copy number aberration (CNA) of stem genes locus. Gene expression was assessed using RT-qPCR. RESULTS: CNA analysis of the studied tumors of patients without chemotherapy showed that 17/18 patients without metastases did not have two or more amplifications of chromosomal regions. Ten patients had visceral metastases. In 9/10 of these patients in the primary tumor there were two or more amplifications of the stem genes locus. Two or more amplifications of stem genes locus were found in 12 patients with stage I. Hematogenous metastases did not develop in all patients. Comparison of metastasis-free survival rates in groups of patients with 1 or without amplifications and with two or more amplifications showed statistically significant differences (P = 0.01). CONCLUSION: Our studies have shown that the presence of clones with two or more amplifications of stem gene in patients with BC T(1)N(x)M(0) has a significant prognostic value and determines an unfavorable prognosis for distant metastasis.
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spelling pubmed-69261012019-12-29 Amplification of Stem Genes: New Potential Metastatic Makers in Patients with an Early Form of Breast Cancer Tsyganov, Matvey M. Ibragimova, Marina K. Pevzner, Alina M. Doroshenko, Artem V. Slonimskaya, Elena M. Litviakov, Nikolai V. J Korean Med Sci Original Article BACKGROUND: According to our previous studies, the presence of amplifications of stem genes can lead to their ectopic expression and this is associated with an increased activity of tumor stem cells in these patients. This leads to a high aggressiveness of the tumor and the development of metastatic disease. The aim was to evaluate the prognostic significance of the presence of amplifications of stem genes and their expression in patients with early breast cancer (BC). METHODS: The study included 28 patients with T(1)N(x)M(0) BC. We used surgical specimens, including formalin-fixed paraffin-embedded archive materials, for 8 patients. A microarray analysis was performed on high-density DNA chips from CytoScanHDArray to assess the status of copy number aberration (CNA) of stem genes locus. Gene expression was assessed using RT-qPCR. RESULTS: CNA analysis of the studied tumors of patients without chemotherapy showed that 17/18 patients without metastases did not have two or more amplifications of chromosomal regions. Ten patients had visceral metastases. In 9/10 of these patients in the primary tumor there were two or more amplifications of the stem genes locus. Two or more amplifications of stem genes locus were found in 12 patients with stage I. Hematogenous metastases did not develop in all patients. Comparison of metastasis-free survival rates in groups of patients with 1 or without amplifications and with two or more amplifications showed statistically significant differences (P = 0.01). CONCLUSION: Our studies have shown that the presence of clones with two or more amplifications of stem gene in patients with BC T(1)N(x)M(0) has a significant prognostic value and determines an unfavorable prognosis for distant metastasis. The Korean Academy of Medical Sciences 2019-11-15 /pmc/articles/PMC6926101/ /pubmed/31858753 http://dx.doi.org/10.3346/jkms.2019.34.e312 Text en © 2019 The Korean Academy of Medical Sciences. https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Tsyganov, Matvey M.
Ibragimova, Marina K.
Pevzner, Alina M.
Doroshenko, Artem V.
Slonimskaya, Elena M.
Litviakov, Nikolai V.
Amplification of Stem Genes: New Potential Metastatic Makers in Patients with an Early Form of Breast Cancer
title Amplification of Stem Genes: New Potential Metastatic Makers in Patients with an Early Form of Breast Cancer
title_full Amplification of Stem Genes: New Potential Metastatic Makers in Patients with an Early Form of Breast Cancer
title_fullStr Amplification of Stem Genes: New Potential Metastatic Makers in Patients with an Early Form of Breast Cancer
title_full_unstemmed Amplification of Stem Genes: New Potential Metastatic Makers in Patients with an Early Form of Breast Cancer
title_short Amplification of Stem Genes: New Potential Metastatic Makers in Patients with an Early Form of Breast Cancer
title_sort amplification of stem genes: new potential metastatic makers in patients with an early form of breast cancer
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6926101/
https://www.ncbi.nlm.nih.gov/pubmed/31858753
http://dx.doi.org/10.3346/jkms.2019.34.e312
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