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A FABP4-PPARγ signaling axis regulates human monocyte responses to electrophilic fatty acid nitroalkenes
Nitro-fatty acids (NO(2)-FA) are electrophilic lipid mediators derived from unsaturated fatty acid nitration. These species are produced endogenously by metabolic and inflammatory reactions and mediate anti-oxidative and anti-inflammatory responses. NO(2)-FA have been postulated as partial agonists...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6926352/ https://www.ncbi.nlm.nih.gov/pubmed/31926616 http://dx.doi.org/10.1016/j.redox.2019.101376 |
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author | Lamas Bervejillo, M. Bonanata, J. Franchini, G.R. Richeri, A. Marqués, J.M. Freeman, B.A. Schopfer, F.J. Coitiño, E.L. Córsico, B. Rubbo, H. Ferreira, A.M. |
author_facet | Lamas Bervejillo, M. Bonanata, J. Franchini, G.R. Richeri, A. Marqués, J.M. Freeman, B.A. Schopfer, F.J. Coitiño, E.L. Córsico, B. Rubbo, H. Ferreira, A.M. |
author_sort | Lamas Bervejillo, M. |
collection | PubMed |
description | Nitro-fatty acids (NO(2)-FA) are electrophilic lipid mediators derived from unsaturated fatty acid nitration. These species are produced endogenously by metabolic and inflammatory reactions and mediate anti-oxidative and anti-inflammatory responses. NO(2)-FA have been postulated as partial agonists of the Peroxisome Proliferator-Activated Receptor gamma (PPARγ), which is predominantly expressed in adipocytes and myeloid cells. Herein, we explored molecular and cellular events associated with PPARγ activation by NO(2)-FA in monocytes and macrophages. NO(2)-FA induced the expression of two PPARγ reporter genes, Fatty Acid Binding Protein 4 (FABP4) and the scavenger receptor CD36, at early stages of monocyte differentiation into macrophages. These responses were inhibited by the specific PPARγ inhibitor GW9662. Attenuated NO(2)-FA effects on PPARγ signaling were observed once cells were differentiated into macrophages, with a significant but lower FABP4 upregulation, and no induction of CD36. Using in vitro and in silico approaches, we demonstrated that NO(2)-FA bind to FABP4. Furthermore, the inhibition of monocyte FA binding by FABP4 diminished NO(2)-FA-induced upregulation of reporter genes that are transcriptionally regulated by PPARγ, Keap1/Nrf2 and HSF1, indicating that FABP4 inhibition mitigates NO(2)-FA signaling actions. Overall, our results affirm that NO(2)-FA activate PPARγ in monocytes and upregulate FABP4 expression, thus promoting a positive amplification loop for the downstream signaling actions of this mediator. |
format | Online Article Text |
id | pubmed-6926352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-69263522019-12-30 A FABP4-PPARγ signaling axis regulates human monocyte responses to electrophilic fatty acid nitroalkenes Lamas Bervejillo, M. Bonanata, J. Franchini, G.R. Richeri, A. Marqués, J.M. Freeman, B.A. Schopfer, F.J. Coitiño, E.L. Córsico, B. Rubbo, H. Ferreira, A.M. Redox Biol Research Paper Nitro-fatty acids (NO(2)-FA) are electrophilic lipid mediators derived from unsaturated fatty acid nitration. These species are produced endogenously by metabolic and inflammatory reactions and mediate anti-oxidative and anti-inflammatory responses. NO(2)-FA have been postulated as partial agonists of the Peroxisome Proliferator-Activated Receptor gamma (PPARγ), which is predominantly expressed in adipocytes and myeloid cells. Herein, we explored molecular and cellular events associated with PPARγ activation by NO(2)-FA in monocytes and macrophages. NO(2)-FA induced the expression of two PPARγ reporter genes, Fatty Acid Binding Protein 4 (FABP4) and the scavenger receptor CD36, at early stages of monocyte differentiation into macrophages. These responses were inhibited by the specific PPARγ inhibitor GW9662. Attenuated NO(2)-FA effects on PPARγ signaling were observed once cells were differentiated into macrophages, with a significant but lower FABP4 upregulation, and no induction of CD36. Using in vitro and in silico approaches, we demonstrated that NO(2)-FA bind to FABP4. Furthermore, the inhibition of monocyte FA binding by FABP4 diminished NO(2)-FA-induced upregulation of reporter genes that are transcriptionally regulated by PPARγ, Keap1/Nrf2 and HSF1, indicating that FABP4 inhibition mitigates NO(2)-FA signaling actions. Overall, our results affirm that NO(2)-FA activate PPARγ in monocytes and upregulate FABP4 expression, thus promoting a positive amplification loop for the downstream signaling actions of this mediator. Elsevier 2019-11-10 /pmc/articles/PMC6926352/ /pubmed/31926616 http://dx.doi.org/10.1016/j.redox.2019.101376 Text en © 2019 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Paper Lamas Bervejillo, M. Bonanata, J. Franchini, G.R. Richeri, A. Marqués, J.M. Freeman, B.A. Schopfer, F.J. Coitiño, E.L. Córsico, B. Rubbo, H. Ferreira, A.M. A FABP4-PPARγ signaling axis regulates human monocyte responses to electrophilic fatty acid nitroalkenes |
title | A FABP4-PPARγ signaling axis regulates human monocyte responses to electrophilic fatty acid nitroalkenes |
title_full | A FABP4-PPARγ signaling axis regulates human monocyte responses to electrophilic fatty acid nitroalkenes |
title_fullStr | A FABP4-PPARγ signaling axis regulates human monocyte responses to electrophilic fatty acid nitroalkenes |
title_full_unstemmed | A FABP4-PPARγ signaling axis regulates human monocyte responses to electrophilic fatty acid nitroalkenes |
title_short | A FABP4-PPARγ signaling axis regulates human monocyte responses to electrophilic fatty acid nitroalkenes |
title_sort | fabp4-pparγ signaling axis regulates human monocyte responses to electrophilic fatty acid nitroalkenes |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6926352/ https://www.ncbi.nlm.nih.gov/pubmed/31926616 http://dx.doi.org/10.1016/j.redox.2019.101376 |
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