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Biologization of Collagen-Based Biomaterials Using Liquid-Platelet-Rich Fibrin: New Insights into Clinically Applicable Tissue Engineering
Platelet-rich fibrin (PRF) is a blood concentrate derived from venous blood that is processed without anticoagulants by a one-step centrifugation process. This three-dimensional scaffold contains inflammatory cells and plasma proteins entrapped in a fibrin matrix. Liquid-PRF was developed based on t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6926831/ https://www.ncbi.nlm.nih.gov/pubmed/31810182 http://dx.doi.org/10.3390/ma12233993 |
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author | Al-Maawi, Sarah Herrera-Vizcaíno, Carlos Orlowska, Anna Willershausen, Ines Sader, Robert Miron, Richard J Choukroun, Joseph Ghanaati, Shahram |
author_facet | Al-Maawi, Sarah Herrera-Vizcaíno, Carlos Orlowska, Anna Willershausen, Ines Sader, Robert Miron, Richard J Choukroun, Joseph Ghanaati, Shahram |
author_sort | Al-Maawi, Sarah |
collection | PubMed |
description | Platelet-rich fibrin (PRF) is a blood concentrate derived from venous blood that is processed without anticoagulants by a one-step centrifugation process. This three-dimensional scaffold contains inflammatory cells and plasma proteins entrapped in a fibrin matrix. Liquid-PRF was developed based on the previously described low-speed centrifuge concept (LSCC), which allowed the introduction of a liquid-PRF formulation of fibrinogen and thrombin prior to its conversion to fibrin. Liquid-PRF was introduced to meet the clinical demand for combination with biomaterials in a clinically applicable and easy-to-use way. The aim of the present study was to evaluate, ex vivo, the interaction of the liquid-PRF constituents with five different collagen biomaterials by histological analyses. The results first demonstrated that large variability existed between the biomaterials investigated. Liquid-PRF was able to completely invade Mucograft(®) (MG; Geistlich Biomaterials, Wolhusen, Switzerland) and to partly invade Bio-Gide(®) (BG; Geistlich Biomaterials, Wolhusen, Switzerland) and Mucoderm(®) (MD; Botiss Biomaterials, Berlin, Germany), and Collprotect(®) (CP; Botiss Biomaterials, Berlin, Germany) showed only a superficial interaction. The BEGO(®) collagen membrane (BCM; BEGO Implant Systems) appeared to be completely free of liquid-PRF. These results were confirmed by the different cellular penetration and liquid-PRF absorption coefficient (PAC) values of the evaluated membranes. The present study demonstrates a system for loading biomaterials with a complex autologous cell system (liquid-PRF) in a relatively short period of time and in a clinically relevant manner. The combination of biomaterials with liquid-PRF may be clinically utilized to enhance the bioactivity of collagen-based biomaterials and may act as a biomaterial-based growth factor delivery system. |
format | Online Article Text |
id | pubmed-6926831 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69268312019-12-23 Biologization of Collagen-Based Biomaterials Using Liquid-Platelet-Rich Fibrin: New Insights into Clinically Applicable Tissue Engineering Al-Maawi, Sarah Herrera-Vizcaíno, Carlos Orlowska, Anna Willershausen, Ines Sader, Robert Miron, Richard J Choukroun, Joseph Ghanaati, Shahram Materials (Basel) Article Platelet-rich fibrin (PRF) is a blood concentrate derived from venous blood that is processed without anticoagulants by a one-step centrifugation process. This three-dimensional scaffold contains inflammatory cells and plasma proteins entrapped in a fibrin matrix. Liquid-PRF was developed based on the previously described low-speed centrifuge concept (LSCC), which allowed the introduction of a liquid-PRF formulation of fibrinogen and thrombin prior to its conversion to fibrin. Liquid-PRF was introduced to meet the clinical demand for combination with biomaterials in a clinically applicable and easy-to-use way. The aim of the present study was to evaluate, ex vivo, the interaction of the liquid-PRF constituents with five different collagen biomaterials by histological analyses. The results first demonstrated that large variability existed between the biomaterials investigated. Liquid-PRF was able to completely invade Mucograft(®) (MG; Geistlich Biomaterials, Wolhusen, Switzerland) and to partly invade Bio-Gide(®) (BG; Geistlich Biomaterials, Wolhusen, Switzerland) and Mucoderm(®) (MD; Botiss Biomaterials, Berlin, Germany), and Collprotect(®) (CP; Botiss Biomaterials, Berlin, Germany) showed only a superficial interaction. The BEGO(®) collagen membrane (BCM; BEGO Implant Systems) appeared to be completely free of liquid-PRF. These results were confirmed by the different cellular penetration and liquid-PRF absorption coefficient (PAC) values of the evaluated membranes. The present study demonstrates a system for loading biomaterials with a complex autologous cell system (liquid-PRF) in a relatively short period of time and in a clinically relevant manner. The combination of biomaterials with liquid-PRF may be clinically utilized to enhance the bioactivity of collagen-based biomaterials and may act as a biomaterial-based growth factor delivery system. MDPI 2019-12-02 /pmc/articles/PMC6926831/ /pubmed/31810182 http://dx.doi.org/10.3390/ma12233993 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Al-Maawi, Sarah Herrera-Vizcaíno, Carlos Orlowska, Anna Willershausen, Ines Sader, Robert Miron, Richard J Choukroun, Joseph Ghanaati, Shahram Biologization of Collagen-Based Biomaterials Using Liquid-Platelet-Rich Fibrin: New Insights into Clinically Applicable Tissue Engineering |
title | Biologization of Collagen-Based Biomaterials Using Liquid-Platelet-Rich Fibrin: New Insights into Clinically Applicable Tissue Engineering |
title_full | Biologization of Collagen-Based Biomaterials Using Liquid-Platelet-Rich Fibrin: New Insights into Clinically Applicable Tissue Engineering |
title_fullStr | Biologization of Collagen-Based Biomaterials Using Liquid-Platelet-Rich Fibrin: New Insights into Clinically Applicable Tissue Engineering |
title_full_unstemmed | Biologization of Collagen-Based Biomaterials Using Liquid-Platelet-Rich Fibrin: New Insights into Clinically Applicable Tissue Engineering |
title_short | Biologization of Collagen-Based Biomaterials Using Liquid-Platelet-Rich Fibrin: New Insights into Clinically Applicable Tissue Engineering |
title_sort | biologization of collagen-based biomaterials using liquid-platelet-rich fibrin: new insights into clinically applicable tissue engineering |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6926831/ https://www.ncbi.nlm.nih.gov/pubmed/31810182 http://dx.doi.org/10.3390/ma12233993 |
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