Cargando…
The chemo-gut study: investigating the long-term effects of chemotherapy on gut microbiota, metabolic, immune, psychological and cognitive parameters in young adult Cancer survivors; study protocol
BACKGROUND: The gut microbiota is an important modulator of immune, metabolic, psychological and cognitive mechanisms. Chemotherapy adversely affects the gut microbiota, inducing acute dysbiosis, and alters physiological and psychological function. Cancer among young adults has risen 38% in recent d...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927187/ https://www.ncbi.nlm.nih.gov/pubmed/31870331 http://dx.doi.org/10.1186/s12885-019-6473-8 |
_version_ | 1783482259742916608 |
---|---|
author | Deleemans, Julie M. Chleilat, Faye Reimer, Raylene A. Henning, Jan-Willem Baydoun, Mohamad Piedalue, Katherine-Ann McLennan, Andrew Carlson, Linda E. |
author_facet | Deleemans, Julie M. Chleilat, Faye Reimer, Raylene A. Henning, Jan-Willem Baydoun, Mohamad Piedalue, Katherine-Ann McLennan, Andrew Carlson, Linda E. |
author_sort | Deleemans, Julie M. |
collection | PubMed |
description | BACKGROUND: The gut microbiota is an important modulator of immune, metabolic, psychological and cognitive mechanisms. Chemotherapy adversely affects the gut microbiota, inducing acute dysbiosis, and alters physiological and psychological function. Cancer among young adults has risen 38% in recent decades. Understanding chemotherapy’s long-term effects on gut microbiota and psycho-physiological function is critical to improve survivors’ physical and mental health, but remains unexamined. Restoration of the gut microbiota via targeted therapies (e.g. probiotics) could potentially prevent or reverse the psycho-physiological deficits often found in young survivors following chemotherapy, ultimately leading to reduced symptom burden and improved health. METHODS: This longitudinal study investigates chemotherapy induced long-term gut dysbiosis, and associations between gut microbiota, and immune, metabolic, cognitive and psychological parameters using data collected at < 2 month (T1), 3–4 months (T2), and 5–6 months (T3) post-chemotherapy. Participants will be 18–39 year old blood or solid tumor cancer survivors (n = 50), and a healthy sibling, partner or friend as a control (n = 50). Gut microbiota composition will be measured from fecal samples using 16 s RNA sequencing. Psychological and cognitive patient reported outcome measures will include depression, anxiety, post-traumatic stress disorder symptoms, pain, fatigue, and social and cognitive function. Dual-energy X-ray Absorptiometry (DXA) will be used to measure fat and lean mass, and bone mineral concentration. Pro-inflammatory cytokines, C-reactive protein (CRP), lipopolysaccharide (LPS), serotonin, and brain derived neurotrophic factor (BDNF) will be measured in serum, and long-term cortisol will be assayed from hair. Regression and linear mixed model (LMM) analyses will examine associations across time points (T1 – T3), between groups, and covariates with gut microbiota, cognitive, psychological, and physiological parameters. CONCLUSION: Knowing what bacterial species are depleted after chemotherapy, how long these effects last, and the physiological mechanisms that may drive psychological and cognitive issues among survivors will allow for targeted, integrative interventions to be developed, helping to prevent or reverse some of the late-effects of treatment that many young cancer survivors face. This protocol has been approved by the Health Research Ethics Board of Alberta Cancer Committee (ID: HREBA.CC-19-0018). |
format | Online Article Text |
id | pubmed-6927187 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-69271872019-12-30 The chemo-gut study: investigating the long-term effects of chemotherapy on gut microbiota, metabolic, immune, psychological and cognitive parameters in young adult Cancer survivors; study protocol Deleemans, Julie M. Chleilat, Faye Reimer, Raylene A. Henning, Jan-Willem Baydoun, Mohamad Piedalue, Katherine-Ann McLennan, Andrew Carlson, Linda E. BMC Cancer Study Protocol BACKGROUND: The gut microbiota is an important modulator of immune, metabolic, psychological and cognitive mechanisms. Chemotherapy adversely affects the gut microbiota, inducing acute dysbiosis, and alters physiological and psychological function. Cancer among young adults has risen 38% in recent decades. Understanding chemotherapy’s long-term effects on gut microbiota and psycho-physiological function is critical to improve survivors’ physical and mental health, but remains unexamined. Restoration of the gut microbiota via targeted therapies (e.g. probiotics) could potentially prevent or reverse the psycho-physiological deficits often found in young survivors following chemotherapy, ultimately leading to reduced symptom burden and improved health. METHODS: This longitudinal study investigates chemotherapy induced long-term gut dysbiosis, and associations between gut microbiota, and immune, metabolic, cognitive and psychological parameters using data collected at < 2 month (T1), 3–4 months (T2), and 5–6 months (T3) post-chemotherapy. Participants will be 18–39 year old blood or solid tumor cancer survivors (n = 50), and a healthy sibling, partner or friend as a control (n = 50). Gut microbiota composition will be measured from fecal samples using 16 s RNA sequencing. Psychological and cognitive patient reported outcome measures will include depression, anxiety, post-traumatic stress disorder symptoms, pain, fatigue, and social and cognitive function. Dual-energy X-ray Absorptiometry (DXA) will be used to measure fat and lean mass, and bone mineral concentration. Pro-inflammatory cytokines, C-reactive protein (CRP), lipopolysaccharide (LPS), serotonin, and brain derived neurotrophic factor (BDNF) will be measured in serum, and long-term cortisol will be assayed from hair. Regression and linear mixed model (LMM) analyses will examine associations across time points (T1 – T3), between groups, and covariates with gut microbiota, cognitive, psychological, and physiological parameters. CONCLUSION: Knowing what bacterial species are depleted after chemotherapy, how long these effects last, and the physiological mechanisms that may drive psychological and cognitive issues among survivors will allow for targeted, integrative interventions to be developed, helping to prevent or reverse some of the late-effects of treatment that many young cancer survivors face. This protocol has been approved by the Health Research Ethics Board of Alberta Cancer Committee (ID: HREBA.CC-19-0018). BioMed Central 2019-12-23 /pmc/articles/PMC6927187/ /pubmed/31870331 http://dx.doi.org/10.1186/s12885-019-6473-8 Text en © The Author(s). 2019 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Study Protocol Deleemans, Julie M. Chleilat, Faye Reimer, Raylene A. Henning, Jan-Willem Baydoun, Mohamad Piedalue, Katherine-Ann McLennan, Andrew Carlson, Linda E. The chemo-gut study: investigating the long-term effects of chemotherapy on gut microbiota, metabolic, immune, psychological and cognitive parameters in young adult Cancer survivors; study protocol |
title | The chemo-gut study: investigating the long-term effects of chemotherapy on gut microbiota, metabolic, immune, psychological and cognitive parameters in young adult Cancer survivors; study protocol |
title_full | The chemo-gut study: investigating the long-term effects of chemotherapy on gut microbiota, metabolic, immune, psychological and cognitive parameters in young adult Cancer survivors; study protocol |
title_fullStr | The chemo-gut study: investigating the long-term effects of chemotherapy on gut microbiota, metabolic, immune, psychological and cognitive parameters in young adult Cancer survivors; study protocol |
title_full_unstemmed | The chemo-gut study: investigating the long-term effects of chemotherapy on gut microbiota, metabolic, immune, psychological and cognitive parameters in young adult Cancer survivors; study protocol |
title_short | The chemo-gut study: investigating the long-term effects of chemotherapy on gut microbiota, metabolic, immune, psychological and cognitive parameters in young adult Cancer survivors; study protocol |
title_sort | chemo-gut study: investigating the long-term effects of chemotherapy on gut microbiota, metabolic, immune, psychological and cognitive parameters in young adult cancer survivors; study protocol |
topic | Study Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927187/ https://www.ncbi.nlm.nih.gov/pubmed/31870331 http://dx.doi.org/10.1186/s12885-019-6473-8 |
work_keys_str_mv | AT deleemansjuliem thechemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT chleilatfaye thechemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT reimerraylenea thechemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT henningjanwillem thechemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT baydounmohamad thechemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT piedaluekatherineann thechemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT mclennanandrew thechemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT carlsonlindae thechemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT deleemansjuliem chemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT chleilatfaye chemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT reimerraylenea chemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT henningjanwillem chemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT baydounmohamad chemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT piedaluekatherineann chemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT mclennanandrew chemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol AT carlsonlindae chemogutstudyinvestigatingthelongtermeffectsofchemotherapyongutmicrobiotametabolicimmunepsychologicalandcognitiveparametersinyoungadultcancersurvivorsstudyprotocol |