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Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope
Microtubules grow not only from the centrosome but also from various noncentrosomal microtubule-organizing centers (MTOCs), including the nuclear envelope (NE) and pre-existing microtubules. The evolutionarily conserved proteins Mto1/CDK5RAP2 and Alp14/TOG/XMAP215 have been shown to be involved in p...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927237/ https://www.ncbi.nlm.nih.gov/pubmed/31087092 http://dx.doi.org/10.1093/jmcb/mjz038 |
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author | Liu, Wenyue Zheng, Fan Wang, Yucai Fu, Chuanhai |
author_facet | Liu, Wenyue Zheng, Fan Wang, Yucai Fu, Chuanhai |
author_sort | Liu, Wenyue |
collection | PubMed |
description | Microtubules grow not only from the centrosome but also from various noncentrosomal microtubule-organizing centers (MTOCs), including the nuclear envelope (NE) and pre-existing microtubules. The evolutionarily conserved proteins Mto1/CDK5RAP2 and Alp14/TOG/XMAP215 have been shown to be involved in promoting microtubule nucleation. However, it has remained elusive as to how the microtubule nucleation promoting factors are specified to various noncentrosomal MTOCs, particularly the NE, and how these proteins coordinate to organize microtubule assembly. Here, we demonstrate that in the fission yeast Schizosaccharomyces pombe, efficient interphase microtubule growth from the NE requires Alp7/TACC, Alp14/TOG/XMAP215, and Mto1/CDK5RAP2. The absence of Alp7, Alp14, or Mto1 compromises microtubule regrowth on the NE in cells undergoing microtubule repolymerization. We further demonstrate that Alp7 and Mto1 interdependently localize to the NE in cells without microtubules and that Alp14 localizes to the NE in an Alp7 and Mto1-dependent manner. Tethering Mto1 to the NE in cells lacking Alp7 partially restores microtubule number and the efficiency of microtubule generation from the NE. Hence, our study delineates that Alp7, Alp14, and Mto1 work in concert to regulate interphase microtubule regrowth on the NE. |
format | Online Article Text |
id | pubmed-6927237 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-69272372019-12-27 Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope Liu, Wenyue Zheng, Fan Wang, Yucai Fu, Chuanhai J Mol Cell Biol Article Microtubules grow not only from the centrosome but also from various noncentrosomal microtubule-organizing centers (MTOCs), including the nuclear envelope (NE) and pre-existing microtubules. The evolutionarily conserved proteins Mto1/CDK5RAP2 and Alp14/TOG/XMAP215 have been shown to be involved in promoting microtubule nucleation. However, it has remained elusive as to how the microtubule nucleation promoting factors are specified to various noncentrosomal MTOCs, particularly the NE, and how these proteins coordinate to organize microtubule assembly. Here, we demonstrate that in the fission yeast Schizosaccharomyces pombe, efficient interphase microtubule growth from the NE requires Alp7/TACC, Alp14/TOG/XMAP215, and Mto1/CDK5RAP2. The absence of Alp7, Alp14, or Mto1 compromises microtubule regrowth on the NE in cells undergoing microtubule repolymerization. We further demonstrate that Alp7 and Mto1 interdependently localize to the NE in cells without microtubules and that Alp14 localizes to the NE in an Alp7 and Mto1-dependent manner. Tethering Mto1 to the NE in cells lacking Alp7 partially restores microtubule number and the efficiency of microtubule generation from the NE. Hence, our study delineates that Alp7, Alp14, and Mto1 work in concert to regulate interphase microtubule regrowth on the NE. Oxford University Press 2019-05-14 /pmc/articles/PMC6927237/ /pubmed/31087092 http://dx.doi.org/10.1093/jmcb/mjz038 Text en © The Author(s) (2019). Published by Oxford University Press on behalf of Journal of Molecular Cell Biology, IBCB, SIBS, CAS. http://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Article Liu, Wenyue Zheng, Fan Wang, Yucai Fu, Chuanhai Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope |
title | Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope |
title_full | Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope |
title_fullStr | Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope |
title_full_unstemmed | Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope |
title_short | Alp7-Mto1 and Alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope |
title_sort | alp7-mto1 and alp14 synergize to promote interphase microtubule regrowth from the nuclear envelope |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927237/ https://www.ncbi.nlm.nih.gov/pubmed/31087092 http://dx.doi.org/10.1093/jmcb/mjz038 |
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