Cargando…

Ionomic and transcriptomic analyses of two cotton cultivars (Gossypium hirsutum L.) provide insights into the ion balance mechanism of cotton under salt stress

Soil salinity is a major abiotic stress factor that limits cotton production worldwide. To improve salt tolerance in cotton, an in-depth understanding of ionic balance is needed. In this study, a pot experiment using three levels of soil salinity (0%, 0.2%, and 0.4%, represented as CK, SL, and SH, r...

Descripción completa

Detalles Bibliográficos
Autores principales: Guo, Huijuan, Li, Shuangnan, Min, Wei, Ye, Jun, Hou, Zhenan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927655/
https://www.ncbi.nlm.nih.gov/pubmed/31869397
http://dx.doi.org/10.1371/journal.pone.0226776
_version_ 1783482339910746112
author Guo, Huijuan
Li, Shuangnan
Min, Wei
Ye, Jun
Hou, Zhenan
author_facet Guo, Huijuan
Li, Shuangnan
Min, Wei
Ye, Jun
Hou, Zhenan
author_sort Guo, Huijuan
collection PubMed
description Soil salinity is a major abiotic stress factor that limits cotton production worldwide. To improve salt tolerance in cotton, an in-depth understanding of ionic balance is needed. In this study, a pot experiment using three levels of soil salinity (0%, 0.2%, and 0.4%, represented as CK, SL, and SH, respectively) and two cotton genotypes (salt-tolerant genotype: L24; salt-sensitive genotype: X45) was employed to investigate how sodium chloride (NaCl) stress effects cotton growth, ion distribution, and transport, as well as to explore the related mechanism. The results showed that SL treatment mainly inhibited shoot growth, while SH treatment caused more extensive impairment to roots and shoots. The growth inhibition ratio of NaCl stress on X45 was more marked than that of L24. Under NaCl stress, the Na concentration in the roots, stems and leaves significantly increased, whereas the K, Cu, B, and Mo concentration in roots, as well as Mg and S concentrations in the leaves, significantly decreased. Under salt stress conditions, salt-tolerant cotton plants can store Na in the leaves, and as a result, a larger amount of minerals (e.g., Cu, Mo, Si, P, and B) tend to transport to the leaves. By contrast, salt-sensitive varieties tend to accumulate certain minerals (e.g., Ca, P, Mg, S, Mn, Fe, Cu, B, Mo, and Si) in the roots. Most genes related to ion transport and homeostasis were upregulated in L24, but not in X45. The expression level of GhSOS1 in X45 was higher than L24, but GhNHX1 in L24 was higher than X45. Our findings suggest that the two varieties response to salt stress differently; for X45 (salt-sensitive), the response is predominantly governed by Na(+) efflux, whereas for L24 (salt-tolerant), vacuolar sequestration of Na(+) is the major mechanism. The expression changes of the genes encoding the ion transporters may partially explain the genotypic difference in leaf ion accumulation under salt stress conditions.
format Online
Article
Text
id pubmed-6927655
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-69276552020-01-07 Ionomic and transcriptomic analyses of two cotton cultivars (Gossypium hirsutum L.) provide insights into the ion balance mechanism of cotton under salt stress Guo, Huijuan Li, Shuangnan Min, Wei Ye, Jun Hou, Zhenan PLoS One Research Article Soil salinity is a major abiotic stress factor that limits cotton production worldwide. To improve salt tolerance in cotton, an in-depth understanding of ionic balance is needed. In this study, a pot experiment using three levels of soil salinity (0%, 0.2%, and 0.4%, represented as CK, SL, and SH, respectively) and two cotton genotypes (salt-tolerant genotype: L24; salt-sensitive genotype: X45) was employed to investigate how sodium chloride (NaCl) stress effects cotton growth, ion distribution, and transport, as well as to explore the related mechanism. The results showed that SL treatment mainly inhibited shoot growth, while SH treatment caused more extensive impairment to roots and shoots. The growth inhibition ratio of NaCl stress on X45 was more marked than that of L24. Under NaCl stress, the Na concentration in the roots, stems and leaves significantly increased, whereas the K, Cu, B, and Mo concentration in roots, as well as Mg and S concentrations in the leaves, significantly decreased. Under salt stress conditions, salt-tolerant cotton plants can store Na in the leaves, and as a result, a larger amount of minerals (e.g., Cu, Mo, Si, P, and B) tend to transport to the leaves. By contrast, salt-sensitive varieties tend to accumulate certain minerals (e.g., Ca, P, Mg, S, Mn, Fe, Cu, B, Mo, and Si) in the roots. Most genes related to ion transport and homeostasis were upregulated in L24, but not in X45. The expression level of GhSOS1 in X45 was higher than L24, but GhNHX1 in L24 was higher than X45. Our findings suggest that the two varieties response to salt stress differently; for X45 (salt-sensitive), the response is predominantly governed by Na(+) efflux, whereas for L24 (salt-tolerant), vacuolar sequestration of Na(+) is the major mechanism. The expression changes of the genes encoding the ion transporters may partially explain the genotypic difference in leaf ion accumulation under salt stress conditions. Public Library of Science 2019-12-23 /pmc/articles/PMC6927655/ /pubmed/31869397 http://dx.doi.org/10.1371/journal.pone.0226776 Text en © 2019 Guo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Guo, Huijuan
Li, Shuangnan
Min, Wei
Ye, Jun
Hou, Zhenan
Ionomic and transcriptomic analyses of two cotton cultivars (Gossypium hirsutum L.) provide insights into the ion balance mechanism of cotton under salt stress
title Ionomic and transcriptomic analyses of two cotton cultivars (Gossypium hirsutum L.) provide insights into the ion balance mechanism of cotton under salt stress
title_full Ionomic and transcriptomic analyses of two cotton cultivars (Gossypium hirsutum L.) provide insights into the ion balance mechanism of cotton under salt stress
title_fullStr Ionomic and transcriptomic analyses of two cotton cultivars (Gossypium hirsutum L.) provide insights into the ion balance mechanism of cotton under salt stress
title_full_unstemmed Ionomic and transcriptomic analyses of two cotton cultivars (Gossypium hirsutum L.) provide insights into the ion balance mechanism of cotton under salt stress
title_short Ionomic and transcriptomic analyses of two cotton cultivars (Gossypium hirsutum L.) provide insights into the ion balance mechanism of cotton under salt stress
title_sort ionomic and transcriptomic analyses of two cotton cultivars (gossypium hirsutum l.) provide insights into the ion balance mechanism of cotton under salt stress
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6927655/
https://www.ncbi.nlm.nih.gov/pubmed/31869397
http://dx.doi.org/10.1371/journal.pone.0226776
work_keys_str_mv AT guohuijuan ionomicandtranscriptomicanalysesoftwocottoncultivarsgossypiumhirsutumlprovideinsightsintotheionbalancemechanismofcottonundersaltstress
AT lishuangnan ionomicandtranscriptomicanalysesoftwocottoncultivarsgossypiumhirsutumlprovideinsightsintotheionbalancemechanismofcottonundersaltstress
AT minwei ionomicandtranscriptomicanalysesoftwocottoncultivarsgossypiumhirsutumlprovideinsightsintotheionbalancemechanismofcottonundersaltstress
AT yejun ionomicandtranscriptomicanalysesoftwocottoncultivarsgossypiumhirsutumlprovideinsightsintotheionbalancemechanismofcottonundersaltstress
AT houzhenan ionomicandtranscriptomicanalysesoftwocottoncultivarsgossypiumhirsutumlprovideinsightsintotheionbalancemechanismofcottonundersaltstress