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An In Vivo Mouse Model of Pelvic Recurrence of Human Colorectal Cancer

Pelvic recurrence of colorectal cancer is a crucial problem because radical surgery can lead to excessive invasion. Novel therapeutic strategies are required instead of surgery. However, there are few suitable models because of the difficulty in transplanting and observing tumors in the pelvis. We h...

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Autores principales: Yamamoto, Masashi, Taniguchi, Kohei, Masubuchi, Shinsuke, Tominaga, Tomo, Inomata, Yosuke, Miyamoto, Akiko, Ishizuka, Taka-Aki, Murakami, Takashi, Osumi, Wataru, Hamamoto, Hiroki, Tanaka, Keitaro, Okuda, Junji, Uchiyama, Kazuhisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928073/
https://www.ncbi.nlm.nih.gov/pubmed/31873140
http://dx.doi.org/10.1038/s41598-019-56152-0
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author Yamamoto, Masashi
Taniguchi, Kohei
Masubuchi, Shinsuke
Tominaga, Tomo
Inomata, Yosuke
Miyamoto, Akiko
Ishizuka, Taka-Aki
Murakami, Takashi
Osumi, Wataru
Hamamoto, Hiroki
Tanaka, Keitaro
Okuda, Junji
Uchiyama, Kazuhisa
author_facet Yamamoto, Masashi
Taniguchi, Kohei
Masubuchi, Shinsuke
Tominaga, Tomo
Inomata, Yosuke
Miyamoto, Akiko
Ishizuka, Taka-Aki
Murakami, Takashi
Osumi, Wataru
Hamamoto, Hiroki
Tanaka, Keitaro
Okuda, Junji
Uchiyama, Kazuhisa
author_sort Yamamoto, Masashi
collection PubMed
description Pelvic recurrence of colorectal cancer is a crucial problem because radical surgery can lead to excessive invasion. Novel therapeutic strategies are required instead of surgery. However, there are few suitable models because of the difficulty in transplanting and observing tumors in the pelvis. We have established an appropriate injection site suitable for the establishment of colorectal cancer pelvic recurrence that allows for the observation of tumor growth. DLD-1 cells stably expressing luciferase (DLD-1 clone#1-Luc) were inoculated into various points of female BALB/c nude mice and the engrafted cells were analyzed with an imaging system employing bioluminescent signals and computed tomography. Weekly analysis with the imaging system showed that a triangular area defined by the vagina, the anus, and the ischial spine was suitable for the engraftment of pelvic tumors. The imaging system was able to detect the engrafted tumor 7 days after the inoculation of cells. Weight loss was observed in our model, and overall survival was 21–42 days. Tumor involvement of adjacent organs was detected histopathologically, as is the case in the clinical situation. These findings suggest that this model is valid for evaluations of the therapeutic effects of novel treatments under development. It is hoped that this model will be used in preclinical research.
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spelling pubmed-69280732019-12-27 An In Vivo Mouse Model of Pelvic Recurrence of Human Colorectal Cancer Yamamoto, Masashi Taniguchi, Kohei Masubuchi, Shinsuke Tominaga, Tomo Inomata, Yosuke Miyamoto, Akiko Ishizuka, Taka-Aki Murakami, Takashi Osumi, Wataru Hamamoto, Hiroki Tanaka, Keitaro Okuda, Junji Uchiyama, Kazuhisa Sci Rep Article Pelvic recurrence of colorectal cancer is a crucial problem because radical surgery can lead to excessive invasion. Novel therapeutic strategies are required instead of surgery. However, there are few suitable models because of the difficulty in transplanting and observing tumors in the pelvis. We have established an appropriate injection site suitable for the establishment of colorectal cancer pelvic recurrence that allows for the observation of tumor growth. DLD-1 cells stably expressing luciferase (DLD-1 clone#1-Luc) were inoculated into various points of female BALB/c nude mice and the engrafted cells were analyzed with an imaging system employing bioluminescent signals and computed tomography. Weekly analysis with the imaging system showed that a triangular area defined by the vagina, the anus, and the ischial spine was suitable for the engraftment of pelvic tumors. The imaging system was able to detect the engrafted tumor 7 days after the inoculation of cells. Weight loss was observed in our model, and overall survival was 21–42 days. Tumor involvement of adjacent organs was detected histopathologically, as is the case in the clinical situation. These findings suggest that this model is valid for evaluations of the therapeutic effects of novel treatments under development. It is hoped that this model will be used in preclinical research. Nature Publishing Group UK 2019-12-23 /pmc/articles/PMC6928073/ /pubmed/31873140 http://dx.doi.org/10.1038/s41598-019-56152-0 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Yamamoto, Masashi
Taniguchi, Kohei
Masubuchi, Shinsuke
Tominaga, Tomo
Inomata, Yosuke
Miyamoto, Akiko
Ishizuka, Taka-Aki
Murakami, Takashi
Osumi, Wataru
Hamamoto, Hiroki
Tanaka, Keitaro
Okuda, Junji
Uchiyama, Kazuhisa
An In Vivo Mouse Model of Pelvic Recurrence of Human Colorectal Cancer
title An In Vivo Mouse Model of Pelvic Recurrence of Human Colorectal Cancer
title_full An In Vivo Mouse Model of Pelvic Recurrence of Human Colorectal Cancer
title_fullStr An In Vivo Mouse Model of Pelvic Recurrence of Human Colorectal Cancer
title_full_unstemmed An In Vivo Mouse Model of Pelvic Recurrence of Human Colorectal Cancer
title_short An In Vivo Mouse Model of Pelvic Recurrence of Human Colorectal Cancer
title_sort in vivo mouse model of pelvic recurrence of human colorectal cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928073/
https://www.ncbi.nlm.nih.gov/pubmed/31873140
http://dx.doi.org/10.1038/s41598-019-56152-0
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