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A Flow Cytometric Method to Quantify the Endosomal Escape of a Protein Toxin to the Cytosol of Target Cells
PURPOSE: The aim of this work was to develop a quantitative, flow cytometric method for tracking the endolysosomal escape of a fluorescently labelled saporin toxin. METHODS: Flow cytometric measurements of fluorescent pulse width and height were used to track the endocytic uptake into Daudi cells of...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Springer US
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928089/ https://www.ncbi.nlm.nih.gov/pubmed/31873810 http://dx.doi.org/10.1007/s11095-019-2725-1 |