Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp

Rapid, sensitive, point-of-care diagnostics are critical for managing infectious diseases. Here we adapt the CRISPR-based SHERLOCK method to develop a rapid, accurate, single copy detection assay for White Spot Syndrome Virus, the most devastating virus impacting global shrimp aquaculture. Further,...

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Autores principales: Sullivan, Timothy J., Dhar, Arun K., Cruz-Flores, Roberto, Bodnar, Andrea G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928230/
https://www.ncbi.nlm.nih.gov/pubmed/31873150
http://dx.doi.org/10.1038/s41598-019-56170-y
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author Sullivan, Timothy J.
Dhar, Arun K.
Cruz-Flores, Roberto
Bodnar, Andrea G.
author_facet Sullivan, Timothy J.
Dhar, Arun K.
Cruz-Flores, Roberto
Bodnar, Andrea G.
author_sort Sullivan, Timothy J.
collection PubMed
description Rapid, sensitive, point-of-care diagnostics are critical for managing infectious diseases. Here we adapt the CRISPR-based SHERLOCK method to develop a rapid, accurate, single copy detection assay for White Spot Syndrome Virus, the most devastating virus impacting global shrimp aquaculture. Further, we combine paper matrix nucleic acid extraction and lateral flow colorimetric reporting to create a fully field-deployable, next-generation diagnostic with potential to transform veterinary pathology, disease ecology, and animal production.
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spelling pubmed-69282302019-12-27 Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp Sullivan, Timothy J. Dhar, Arun K. Cruz-Flores, Roberto Bodnar, Andrea G. Sci Rep Article Rapid, sensitive, point-of-care diagnostics are critical for managing infectious diseases. Here we adapt the CRISPR-based SHERLOCK method to develop a rapid, accurate, single copy detection assay for White Spot Syndrome Virus, the most devastating virus impacting global shrimp aquaculture. Further, we combine paper matrix nucleic acid extraction and lateral flow colorimetric reporting to create a fully field-deployable, next-generation diagnostic with potential to transform veterinary pathology, disease ecology, and animal production. Nature Publishing Group UK 2019-12-23 /pmc/articles/PMC6928230/ /pubmed/31873150 http://dx.doi.org/10.1038/s41598-019-56170-y Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Sullivan, Timothy J.
Dhar, Arun K.
Cruz-Flores, Roberto
Bodnar, Andrea G.
Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp
title Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp
title_full Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp
title_fullStr Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp
title_full_unstemmed Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp
title_short Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp
title_sort rapid, crispr-based, field-deployable detection of white spot syndrome virus in shrimp
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928230/
https://www.ncbi.nlm.nih.gov/pubmed/31873150
http://dx.doi.org/10.1038/s41598-019-56170-y
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