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Mitochondrial respiration of complex II is not lower than that of complex I in mouse skeletal muscle

Skeletal muscle (SKM) requires a large amount of energy, which is produced mainly by mitochondria, for their daily functioning. Of the several mitochondrial complexes, it has been reported that the dysfunction of complex II is associated with several diseases, including myopathy. However, the degree...

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Autores principales: Maekawa, Satoshi, Takada, Shingo, Furihata, Takaaki, Fukushima, Arata, Yokota, Takashi, Kinugawa, Shintaro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928343/
https://www.ncbi.nlm.nih.gov/pubmed/31890905
http://dx.doi.org/10.1016/j.bbrep.2019.100717
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author Maekawa, Satoshi
Takada, Shingo
Furihata, Takaaki
Fukushima, Arata
Yokota, Takashi
Kinugawa, Shintaro
author_facet Maekawa, Satoshi
Takada, Shingo
Furihata, Takaaki
Fukushima, Arata
Yokota, Takashi
Kinugawa, Shintaro
author_sort Maekawa, Satoshi
collection PubMed
description Skeletal muscle (SKM) requires a large amount of energy, which is produced mainly by mitochondria, for their daily functioning. Of the several mitochondrial complexes, it has been reported that the dysfunction of complex II is associated with several diseases, including myopathy. However, the degree to which complex II contributes to ATP production by mitochondria remains unknown. As complex II is not included in supercomplexes, which are formed to produce ATP efficiently, we hypothesized that complex II-linked respiration was lower than that of complex I. In addition, differences in the characteristics of complex I and II activity suggest that different factors might regulate their function. The isolated mitochondria from gastrocnemius muscle was used for mitochondrial respiration measurement and immunoblotting in male C57BL/6J mice. Student paired t-tests were performed to compare means between two groups. A univariate linear regression model was used to determine the correlation between mitochondrial respiration and proteins. Contrary to our hypothesis, complex II-linked respiration was not significantly less than complex I-linked respiration in SKM mitochondria (complex I vs complex II, 3402 vs 2840 pmol/[s × mg]). Complex I-linked respiration correlated with the amount of complex I incorporated in supercomplexes (r = 0.727, p < 0.05), but not with the total amount of complex I subunits. In contrast, complex II-linked respiration correlated with the total amount of complex II (r = 0.883, p < 0.05), but not with the amount of each complex II subunit. We conclude that both complex I and II play important roles in mitochondrial respiration and that the assembly of both supercomplexes and complex II is essential for the normal functioning of complex I and II in mouse SKM mitochondria.
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spelling pubmed-69283432019-12-30 Mitochondrial respiration of complex II is not lower than that of complex I in mouse skeletal muscle Maekawa, Satoshi Takada, Shingo Furihata, Takaaki Fukushima, Arata Yokota, Takashi Kinugawa, Shintaro Biochem Biophys Rep Research Article Skeletal muscle (SKM) requires a large amount of energy, which is produced mainly by mitochondria, for their daily functioning. Of the several mitochondrial complexes, it has been reported that the dysfunction of complex II is associated with several diseases, including myopathy. However, the degree to which complex II contributes to ATP production by mitochondria remains unknown. As complex II is not included in supercomplexes, which are formed to produce ATP efficiently, we hypothesized that complex II-linked respiration was lower than that of complex I. In addition, differences in the characteristics of complex I and II activity suggest that different factors might regulate their function. The isolated mitochondria from gastrocnemius muscle was used for mitochondrial respiration measurement and immunoblotting in male C57BL/6J mice. Student paired t-tests were performed to compare means between two groups. A univariate linear regression model was used to determine the correlation between mitochondrial respiration and proteins. Contrary to our hypothesis, complex II-linked respiration was not significantly less than complex I-linked respiration in SKM mitochondria (complex I vs complex II, 3402 vs 2840 pmol/[s × mg]). Complex I-linked respiration correlated with the amount of complex I incorporated in supercomplexes (r = 0.727, p < 0.05), but not with the total amount of complex I subunits. In contrast, complex II-linked respiration correlated with the total amount of complex II (r = 0.883, p < 0.05), but not with the amount of each complex II subunit. We conclude that both complex I and II play important roles in mitochondrial respiration and that the assembly of both supercomplexes and complex II is essential for the normal functioning of complex I and II in mouse SKM mitochondria. Elsevier 2019-12-18 /pmc/articles/PMC6928343/ /pubmed/31890905 http://dx.doi.org/10.1016/j.bbrep.2019.100717 Text en © 2019 Published by Elsevier B.V. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Maekawa, Satoshi
Takada, Shingo
Furihata, Takaaki
Fukushima, Arata
Yokota, Takashi
Kinugawa, Shintaro
Mitochondrial respiration of complex II is not lower than that of complex I in mouse skeletal muscle
title Mitochondrial respiration of complex II is not lower than that of complex I in mouse skeletal muscle
title_full Mitochondrial respiration of complex II is not lower than that of complex I in mouse skeletal muscle
title_fullStr Mitochondrial respiration of complex II is not lower than that of complex I in mouse skeletal muscle
title_full_unstemmed Mitochondrial respiration of complex II is not lower than that of complex I in mouse skeletal muscle
title_short Mitochondrial respiration of complex II is not lower than that of complex I in mouse skeletal muscle
title_sort mitochondrial respiration of complex ii is not lower than that of complex i in mouse skeletal muscle
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928343/
https://www.ncbi.nlm.nih.gov/pubmed/31890905
http://dx.doi.org/10.1016/j.bbrep.2019.100717
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