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Effects of Hyperoxia on the Refraction in Murine Neonatal and Adult Models

Whether hyperoxia affects the refraction in neonatal and adult mice is unknown. The mice exposed to 85% oxygen at postnatal 8 days (P8d) for 3 days and the mice exposed to normal air were assigned to the neonatal hyperoxia and normoxia groups, respectively. The refraction, the corneal curvature radi...

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Autores principales: Mori, Kiwako, Kurihara, Toshihide, Jiang, Xiaoyan, Ikeda, Shin-ichi, Ishida, Ayako, Torii, Hidemasa, Tsubota, Kazuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928741/
https://www.ncbi.nlm.nih.gov/pubmed/31795325
http://dx.doi.org/10.3390/ijms20236014
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author Mori, Kiwako
Kurihara, Toshihide
Jiang, Xiaoyan
Ikeda, Shin-ichi
Ishida, Ayako
Torii, Hidemasa
Tsubota, Kazuo
author_facet Mori, Kiwako
Kurihara, Toshihide
Jiang, Xiaoyan
Ikeda, Shin-ichi
Ishida, Ayako
Torii, Hidemasa
Tsubota, Kazuo
author_sort Mori, Kiwako
collection PubMed
description Whether hyperoxia affects the refraction in neonatal and adult mice is unknown. The mice exposed to 85% oxygen at postnatal 8 days (P8d) for 3 days and the mice exposed to normal air were assigned to the neonatal hyperoxia and normoxia groups, respectively. The refraction, the corneal curvature radius (CR) and the axial length (AL) were measured at P30d and P47d. Postnatal 6 weeks (P6w) adult mice were divided into the adult hyperoxia and normoxia groups. These parameters were measured before oxygen exposure, after 1 and 6 weeks, and every 7 weeks. The lens elasticity was measured at P7w and P26w by enucleation. The neonatal hyperoxia group showed a significantly larger myopic change than the neonatal normoxia group (P47d −6.56 ± 5.89 D, +4.11 ± 2.02 D, p < 0.001), whereas the changes in AL were not significantly different (P47d, 3.31 ± 0.04 mm, 3.31 ± 0.05 mm, p = 0.852). The adult hyperoxia group also showed a significantly larger myopic change (P12w, −7.20 ± 4.09 D, +7.52 ± 2.54 D, p < 0.001). The AL did not show significant difference (P12w, 3.44 ± 0.03 mm, 3.43 ± 0.01 mm, p = 0.545); however, the CR in the adult hyperoxia group was significantly smaller than the adult normoxia group (P12w, 1.44 ± 0.03 mm, 1.50 ± 0.03 mm, p = 0.003). In conclusion, hyperoxia was demonstrated to induce myopic shift both in neonatal and adult mice, which was attributed to the change in the CR rather than the AL. Elucidation of the mechanisms of hyperoxia and the application of this result to humans should be carried out in future studies.
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spelling pubmed-69287412019-12-26 Effects of Hyperoxia on the Refraction in Murine Neonatal and Adult Models Mori, Kiwako Kurihara, Toshihide Jiang, Xiaoyan Ikeda, Shin-ichi Ishida, Ayako Torii, Hidemasa Tsubota, Kazuo Int J Mol Sci Article Whether hyperoxia affects the refraction in neonatal and adult mice is unknown. The mice exposed to 85% oxygen at postnatal 8 days (P8d) for 3 days and the mice exposed to normal air were assigned to the neonatal hyperoxia and normoxia groups, respectively. The refraction, the corneal curvature radius (CR) and the axial length (AL) were measured at P30d and P47d. Postnatal 6 weeks (P6w) adult mice were divided into the adult hyperoxia and normoxia groups. These parameters were measured before oxygen exposure, after 1 and 6 weeks, and every 7 weeks. The lens elasticity was measured at P7w and P26w by enucleation. The neonatal hyperoxia group showed a significantly larger myopic change than the neonatal normoxia group (P47d −6.56 ± 5.89 D, +4.11 ± 2.02 D, p < 0.001), whereas the changes in AL were not significantly different (P47d, 3.31 ± 0.04 mm, 3.31 ± 0.05 mm, p = 0.852). The adult hyperoxia group also showed a significantly larger myopic change (P12w, −7.20 ± 4.09 D, +7.52 ± 2.54 D, p < 0.001). The AL did not show significant difference (P12w, 3.44 ± 0.03 mm, 3.43 ± 0.01 mm, p = 0.545); however, the CR in the adult hyperoxia group was significantly smaller than the adult normoxia group (P12w, 1.44 ± 0.03 mm, 1.50 ± 0.03 mm, p = 0.003). In conclusion, hyperoxia was demonstrated to induce myopic shift both in neonatal and adult mice, which was attributed to the change in the CR rather than the AL. Elucidation of the mechanisms of hyperoxia and the application of this result to humans should be carried out in future studies. MDPI 2019-11-29 /pmc/articles/PMC6928741/ /pubmed/31795325 http://dx.doi.org/10.3390/ijms20236014 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mori, Kiwako
Kurihara, Toshihide
Jiang, Xiaoyan
Ikeda, Shin-ichi
Ishida, Ayako
Torii, Hidemasa
Tsubota, Kazuo
Effects of Hyperoxia on the Refraction in Murine Neonatal and Adult Models
title Effects of Hyperoxia on the Refraction in Murine Neonatal and Adult Models
title_full Effects of Hyperoxia on the Refraction in Murine Neonatal and Adult Models
title_fullStr Effects of Hyperoxia on the Refraction in Murine Neonatal and Adult Models
title_full_unstemmed Effects of Hyperoxia on the Refraction in Murine Neonatal and Adult Models
title_short Effects of Hyperoxia on the Refraction in Murine Neonatal and Adult Models
title_sort effects of hyperoxia on the refraction in murine neonatal and adult models
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928741/
https://www.ncbi.nlm.nih.gov/pubmed/31795325
http://dx.doi.org/10.3390/ijms20236014
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