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The Role of EjSVPs in Flower Initiation in Eriobotrya japonica

Flowering plants have evolved different flowering habits to sustain long-term reproduction. Most woody trees experience dormancy and then bloom in the warm spring, but loquat blooms in the cold autumn and winter. To explore its mechanism of flowering regulation, we cloned two SHORT VEGETATIVE PHASE...

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Autores principales: Jiang, Yuanyuan, Peng, Jiangrong, Zhang, Zhike, Lin, Shoukai, Lin, Shunquan, Yang, Xianghui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928820/
https://www.ncbi.nlm.nih.gov/pubmed/31779080
http://dx.doi.org/10.3390/ijms20235933
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author Jiang, Yuanyuan
Peng, Jiangrong
Zhang, Zhike
Lin, Shoukai
Lin, Shunquan
Yang, Xianghui
author_facet Jiang, Yuanyuan
Peng, Jiangrong
Zhang, Zhike
Lin, Shoukai
Lin, Shunquan
Yang, Xianghui
author_sort Jiang, Yuanyuan
collection PubMed
description Flowering plants have evolved different flowering habits to sustain long-term reproduction. Most woody trees experience dormancy and then bloom in the warm spring, but loquat blooms in the cold autumn and winter. To explore its mechanism of flowering regulation, we cloned two SHORT VEGETATIVE PHASE (SVP) homologous genes from ‘Jiefanzhong’ loquat (Eriobotrya japonica Lindl.), namely, EjSVP1 and EjSVP2. Sequence analysis revealed that the EjSVPs were typical MADS-box transcription factors and exhibited a close genetic relationship with other plant SVP/DORMANCY-ASSOCIATED MADS-BOX (DAM) proteins. The temporal and spatial expression patterns showed that EjSVP1 and EjSVP2 were mainly expressed in the shoot apical meristem (SAM) after the initiation of flowering; after reaching their highest level, they gradually decreased with the development of the flower until they could not be detected. EjSVP1 expression levels were relatively high in young tissues, and EjSVP2 expression levels were relatively high in young to mature transformed tissues. Interestingly, EjSVP2 showed relatively high expression levels in various flower tissues. We analyzed the EjSVP promoter regions and found that they did not contain the C-repeat/dehydration-responsive element. Finally, we overexpressed the EjSVPs in wild-type Arabidopsis thaliana Col-0 and found no significant changes in the number of rosette leaves of Arabidopsis thaliana; however, overexpression of EjSVP2 affected the formation of Arabidopsis thaliana flower organs. In conclusion, EjSVPs were found to play an active role in the development of loquat flowering. These findings may provide a reference for exploring the regulation mechanisms of loquat flowering and the dormancy mechanisms of other plants.
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spelling pubmed-69288202019-12-26 The Role of EjSVPs in Flower Initiation in Eriobotrya japonica Jiang, Yuanyuan Peng, Jiangrong Zhang, Zhike Lin, Shoukai Lin, Shunquan Yang, Xianghui Int J Mol Sci Article Flowering plants have evolved different flowering habits to sustain long-term reproduction. Most woody trees experience dormancy and then bloom in the warm spring, but loquat blooms in the cold autumn and winter. To explore its mechanism of flowering regulation, we cloned two SHORT VEGETATIVE PHASE (SVP) homologous genes from ‘Jiefanzhong’ loquat (Eriobotrya japonica Lindl.), namely, EjSVP1 and EjSVP2. Sequence analysis revealed that the EjSVPs were typical MADS-box transcription factors and exhibited a close genetic relationship with other plant SVP/DORMANCY-ASSOCIATED MADS-BOX (DAM) proteins. The temporal and spatial expression patterns showed that EjSVP1 and EjSVP2 were mainly expressed in the shoot apical meristem (SAM) after the initiation of flowering; after reaching their highest level, they gradually decreased with the development of the flower until they could not be detected. EjSVP1 expression levels were relatively high in young tissues, and EjSVP2 expression levels were relatively high in young to mature transformed tissues. Interestingly, EjSVP2 showed relatively high expression levels in various flower tissues. We analyzed the EjSVP promoter regions and found that they did not contain the C-repeat/dehydration-responsive element. Finally, we overexpressed the EjSVPs in wild-type Arabidopsis thaliana Col-0 and found no significant changes in the number of rosette leaves of Arabidopsis thaliana; however, overexpression of EjSVP2 affected the formation of Arabidopsis thaliana flower organs. In conclusion, EjSVPs were found to play an active role in the development of loquat flowering. These findings may provide a reference for exploring the regulation mechanisms of loquat flowering and the dormancy mechanisms of other plants. MDPI 2019-11-26 /pmc/articles/PMC6928820/ /pubmed/31779080 http://dx.doi.org/10.3390/ijms20235933 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jiang, Yuanyuan
Peng, Jiangrong
Zhang, Zhike
Lin, Shoukai
Lin, Shunquan
Yang, Xianghui
The Role of EjSVPs in Flower Initiation in Eriobotrya japonica
title The Role of EjSVPs in Flower Initiation in Eriobotrya japonica
title_full The Role of EjSVPs in Flower Initiation in Eriobotrya japonica
title_fullStr The Role of EjSVPs in Flower Initiation in Eriobotrya japonica
title_full_unstemmed The Role of EjSVPs in Flower Initiation in Eriobotrya japonica
title_short The Role of EjSVPs in Flower Initiation in Eriobotrya japonica
title_sort role of ejsvps in flower initiation in eriobotrya japonica
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928820/
https://www.ncbi.nlm.nih.gov/pubmed/31779080
http://dx.doi.org/10.3390/ijms20235933
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