Cargando…

The Omega-3 Fatty Acids EPA and DHA, as a Part of a Murine High-Fat Diet, Reduced Lipid Accumulation in Brown and White Adipose Tissues

Excess energy intake can trigger an uncontrolled inflammatory response, leading to systemic low-grade inflammation and metabolic disturbances that are hypothesised to contribute to cardiovascular disease and type 2 diabetes. The long chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs) eicosapentaen...

Descripción completa

Detalles Bibliográficos
Autores principales: Soni, Nikul, Ross, Alastair B., Scheers, Nathalie, Nookaew, Intawat, Gabrielsson, Britt G., Sandberg, Ann-Sofie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928976/
https://www.ncbi.nlm.nih.gov/pubmed/31771283
http://dx.doi.org/10.3390/ijms20235895
_version_ 1783482597464080384
author Soni, Nikul
Ross, Alastair B.
Scheers, Nathalie
Nookaew, Intawat
Gabrielsson, Britt G.
Sandberg, Ann-Sofie
author_facet Soni, Nikul
Ross, Alastair B.
Scheers, Nathalie
Nookaew, Intawat
Gabrielsson, Britt G.
Sandberg, Ann-Sofie
author_sort Soni, Nikul
collection PubMed
description Excess energy intake can trigger an uncontrolled inflammatory response, leading to systemic low-grade inflammation and metabolic disturbances that are hypothesised to contribute to cardiovascular disease and type 2 diabetes. The long chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are suggested to mitigate this inflammatory response, but the mechanisms are unclear, especially at the tissue level. Adipose tissues, the first tissues to give an inflammatory response, may be an important target site of action for EPA and DHA. To evaluate the effects of EPA and DHA in white and brown adipose tissues, we fed male C57Bl/6J mice either a high fat diet (HFD) with 5% corn oil, an HFD with 40% of the corn oil substituted for purified EPA and DHA triglycerides (HFD-ED), or normal chow, for 8 weeks. Fatty acid profiling and transcriptomics were used to study how EPA and DHA affect retroperitoneal white and brown adipose tissues. HFD-ED fed mice showed reduced lipid accumulation and levels of the pro-inflammatory fatty acid arachidonic acid in both white and brown adipose tissues, compared with HFD-corn oil fed animals. The transcriptomic analysis showed changes in β-oxidation pathways, supporting the decreased lipid accumulation in the HFD-ED fed mice. Therefore, our data suggests that EPA and DHA supplementation of a high fat diet may be anti-inflammatory, as well as reduce lipid accumulation in adipose tissues.
format Online
Article
Text
id pubmed-6928976
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-69289762019-12-26 The Omega-3 Fatty Acids EPA and DHA, as a Part of a Murine High-Fat Diet, Reduced Lipid Accumulation in Brown and White Adipose Tissues Soni, Nikul Ross, Alastair B. Scheers, Nathalie Nookaew, Intawat Gabrielsson, Britt G. Sandberg, Ann-Sofie Int J Mol Sci Article Excess energy intake can trigger an uncontrolled inflammatory response, leading to systemic low-grade inflammation and metabolic disturbances that are hypothesised to contribute to cardiovascular disease and type 2 diabetes. The long chain n-3 polyunsaturated fatty acids (LC n-3 PUFAs) eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are suggested to mitigate this inflammatory response, but the mechanisms are unclear, especially at the tissue level. Adipose tissues, the first tissues to give an inflammatory response, may be an important target site of action for EPA and DHA. To evaluate the effects of EPA and DHA in white and brown adipose tissues, we fed male C57Bl/6J mice either a high fat diet (HFD) with 5% corn oil, an HFD with 40% of the corn oil substituted for purified EPA and DHA triglycerides (HFD-ED), or normal chow, for 8 weeks. Fatty acid profiling and transcriptomics were used to study how EPA and DHA affect retroperitoneal white and brown adipose tissues. HFD-ED fed mice showed reduced lipid accumulation and levels of the pro-inflammatory fatty acid arachidonic acid in both white and brown adipose tissues, compared with HFD-corn oil fed animals. The transcriptomic analysis showed changes in β-oxidation pathways, supporting the decreased lipid accumulation in the HFD-ED fed mice. Therefore, our data suggests that EPA and DHA supplementation of a high fat diet may be anti-inflammatory, as well as reduce lipid accumulation in adipose tissues. MDPI 2019-11-24 /pmc/articles/PMC6928976/ /pubmed/31771283 http://dx.doi.org/10.3390/ijms20235895 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Soni, Nikul
Ross, Alastair B.
Scheers, Nathalie
Nookaew, Intawat
Gabrielsson, Britt G.
Sandberg, Ann-Sofie
The Omega-3 Fatty Acids EPA and DHA, as a Part of a Murine High-Fat Diet, Reduced Lipid Accumulation in Brown and White Adipose Tissues
title The Omega-3 Fatty Acids EPA and DHA, as a Part of a Murine High-Fat Diet, Reduced Lipid Accumulation in Brown and White Adipose Tissues
title_full The Omega-3 Fatty Acids EPA and DHA, as a Part of a Murine High-Fat Diet, Reduced Lipid Accumulation in Brown and White Adipose Tissues
title_fullStr The Omega-3 Fatty Acids EPA and DHA, as a Part of a Murine High-Fat Diet, Reduced Lipid Accumulation in Brown and White Adipose Tissues
title_full_unstemmed The Omega-3 Fatty Acids EPA and DHA, as a Part of a Murine High-Fat Diet, Reduced Lipid Accumulation in Brown and White Adipose Tissues
title_short The Omega-3 Fatty Acids EPA and DHA, as a Part of a Murine High-Fat Diet, Reduced Lipid Accumulation in Brown and White Adipose Tissues
title_sort omega-3 fatty acids epa and dha, as a part of a murine high-fat diet, reduced lipid accumulation in brown and white adipose tissues
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6928976/
https://www.ncbi.nlm.nih.gov/pubmed/31771283
http://dx.doi.org/10.3390/ijms20235895
work_keys_str_mv AT soninikul theomega3fattyacidsepaanddhaasapartofamurinehighfatdietreducedlipidaccumulationinbrownandwhiteadiposetissues
AT rossalastairb theomega3fattyacidsepaanddhaasapartofamurinehighfatdietreducedlipidaccumulationinbrownandwhiteadiposetissues
AT scheersnathalie theomega3fattyacidsepaanddhaasapartofamurinehighfatdietreducedlipidaccumulationinbrownandwhiteadiposetissues
AT nookaewintawat theomega3fattyacidsepaanddhaasapartofamurinehighfatdietreducedlipidaccumulationinbrownandwhiteadiposetissues
AT gabrielssonbrittg theomega3fattyacidsepaanddhaasapartofamurinehighfatdietreducedlipidaccumulationinbrownandwhiteadiposetissues
AT sandbergannsofie theomega3fattyacidsepaanddhaasapartofamurinehighfatdietreducedlipidaccumulationinbrownandwhiteadiposetissues
AT soninikul omega3fattyacidsepaanddhaasapartofamurinehighfatdietreducedlipidaccumulationinbrownandwhiteadiposetissues
AT rossalastairb omega3fattyacidsepaanddhaasapartofamurinehighfatdietreducedlipidaccumulationinbrownandwhiteadiposetissues
AT scheersnathalie omega3fattyacidsepaanddhaasapartofamurinehighfatdietreducedlipidaccumulationinbrownandwhiteadiposetissues
AT nookaewintawat omega3fattyacidsepaanddhaasapartofamurinehighfatdietreducedlipidaccumulationinbrownandwhiteadiposetissues
AT gabrielssonbrittg omega3fattyacidsepaanddhaasapartofamurinehighfatdietreducedlipidaccumulationinbrownandwhiteadiposetissues
AT sandbergannsofie omega3fattyacidsepaanddhaasapartofamurinehighfatdietreducedlipidaccumulationinbrownandwhiteadiposetissues