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Crystal Structure of a GH3 β-Glucosidase from the Thermophilic Fungus Chaetomium thermophilum
Beta-glucosidases (β-glucosidases) have attracted considerable attention in recent years for use in various biotechnological applications. They are also essential enzymes for lignocellulose degradation in biofuel production. However, cost-effective biomass conversion requires the use of highly effic...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929035/ https://www.ncbi.nlm.nih.gov/pubmed/31783503 http://dx.doi.org/10.3390/ijms20235962 |
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author | Mohsin, Imran Poudel, Nirmal Li, Duo-Chuan Papageorgiou, Anastassios C. |
author_facet | Mohsin, Imran Poudel, Nirmal Li, Duo-Chuan Papageorgiou, Anastassios C. |
author_sort | Mohsin, Imran |
collection | PubMed |
description | Beta-glucosidases (β-glucosidases) have attracted considerable attention in recent years for use in various biotechnological applications. They are also essential enzymes for lignocellulose degradation in biofuel production. However, cost-effective biomass conversion requires the use of highly efficient enzymes. Thus, the search for new enzymes as better alternatives of the currently available enzyme preparations is highly important. Thermophilic fungi are nowadays considered as a promising source of enzymes with improved stability. Here, the crystal structure of a family GH3 β-glucosidase from the thermophilic fungus Chaetomium thermophilum (CtBGL) was determined at a resolution of 2.99 Å. The structure showed the three-domain architecture found in other β-glucosidases with variations in loops and linker regions. The active site catalytic residues in CtBGL were identified as Asp287 (nucleophile) and Glu517 (acid/base). Structural comparison of CtBGL with Protein Data Bank (PDB)-deposited structures revealed variations among glycosylated Asn residues. The enzyme displayed moderate glycosylation compared to other GH3 family β-glucosidases with similar structure. A new glycosylation site at position Asn504 was identified in CtBGL. Moreover, comparison with respect to several thermostability parameters suggested that glycosylation and charged residues involved in electrostatic interactions may contribute to the stability of the enzyme at elevated temperatures. The reported CtBGL structure provides additional insights into the family GH3 enzymes and could offer new ideas for further improvements in β-glucosidases for more efficient use in biotechnological applications regarding cellulose degradation. |
format | Online Article Text |
id | pubmed-6929035 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-69290352019-12-26 Crystal Structure of a GH3 β-Glucosidase from the Thermophilic Fungus Chaetomium thermophilum Mohsin, Imran Poudel, Nirmal Li, Duo-Chuan Papageorgiou, Anastassios C. Int J Mol Sci Article Beta-glucosidases (β-glucosidases) have attracted considerable attention in recent years for use in various biotechnological applications. They are also essential enzymes for lignocellulose degradation in biofuel production. However, cost-effective biomass conversion requires the use of highly efficient enzymes. Thus, the search for new enzymes as better alternatives of the currently available enzyme preparations is highly important. Thermophilic fungi are nowadays considered as a promising source of enzymes with improved stability. Here, the crystal structure of a family GH3 β-glucosidase from the thermophilic fungus Chaetomium thermophilum (CtBGL) was determined at a resolution of 2.99 Å. The structure showed the three-domain architecture found in other β-glucosidases with variations in loops and linker regions. The active site catalytic residues in CtBGL were identified as Asp287 (nucleophile) and Glu517 (acid/base). Structural comparison of CtBGL with Protein Data Bank (PDB)-deposited structures revealed variations among glycosylated Asn residues. The enzyme displayed moderate glycosylation compared to other GH3 family β-glucosidases with similar structure. A new glycosylation site at position Asn504 was identified in CtBGL. Moreover, comparison with respect to several thermostability parameters suggested that glycosylation and charged residues involved in electrostatic interactions may contribute to the stability of the enzyme at elevated temperatures. The reported CtBGL structure provides additional insights into the family GH3 enzymes and could offer new ideas for further improvements in β-glucosidases for more efficient use in biotechnological applications regarding cellulose degradation. MDPI 2019-11-27 /pmc/articles/PMC6929035/ /pubmed/31783503 http://dx.doi.org/10.3390/ijms20235962 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mohsin, Imran Poudel, Nirmal Li, Duo-Chuan Papageorgiou, Anastassios C. Crystal Structure of a GH3 β-Glucosidase from the Thermophilic Fungus Chaetomium thermophilum |
title | Crystal Structure of a GH3 β-Glucosidase from the Thermophilic Fungus Chaetomium thermophilum |
title_full | Crystal Structure of a GH3 β-Glucosidase from the Thermophilic Fungus Chaetomium thermophilum |
title_fullStr | Crystal Structure of a GH3 β-Glucosidase from the Thermophilic Fungus Chaetomium thermophilum |
title_full_unstemmed | Crystal Structure of a GH3 β-Glucosidase from the Thermophilic Fungus Chaetomium thermophilum |
title_short | Crystal Structure of a GH3 β-Glucosidase from the Thermophilic Fungus Chaetomium thermophilum |
title_sort | crystal structure of a gh3 β-glucosidase from the thermophilic fungus chaetomium thermophilum |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929035/ https://www.ncbi.nlm.nih.gov/pubmed/31783503 http://dx.doi.org/10.3390/ijms20235962 |
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