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The Precursor for Nerve Growth Factor (proNGF) in Thyroid Cancer Lymph Node Metastases: Correlation with Primary Tumour and Pathological Variables

Metastases in thyroid cancer are associated with aggressive disease and increased patient morbidity, but the factors driving metastatic progression are unclear. The precursor for nerve growth factor (proNGF) is increased in primary thyroid cancers, but its expression or significance in metastases is...

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Autores principales: Rowe, Christopher W., Dill, Tony, Faulkner, Sam, Gedye, Craig, Paul, Jonathan W., Tolosa, Jorge M., Jones, Mark, King, Simon, Smith, Roger, Hondermarck, Hubert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929117/
https://www.ncbi.nlm.nih.gov/pubmed/31775361
http://dx.doi.org/10.3390/ijms20235924
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author Rowe, Christopher W.
Dill, Tony
Faulkner, Sam
Gedye, Craig
Paul, Jonathan W.
Tolosa, Jorge M.
Jones, Mark
King, Simon
Smith, Roger
Hondermarck, Hubert
author_facet Rowe, Christopher W.
Dill, Tony
Faulkner, Sam
Gedye, Craig
Paul, Jonathan W.
Tolosa, Jorge M.
Jones, Mark
King, Simon
Smith, Roger
Hondermarck, Hubert
author_sort Rowe, Christopher W.
collection PubMed
description Metastases in thyroid cancer are associated with aggressive disease and increased patient morbidity, but the factors driving metastatic progression are unclear. The precursor for nerve growth factor (proNGF) is increased in primary thyroid cancers, but its expression or significance in metastases is not known. In this study, we analysed the expression of proNGF in a retrospective cohort of thyroid cancer lymph node metastases (n = 56), linked with corresponding primary tumours, by automated immunohistochemistry and digital quantification. Potential associations of proNGF immunostaining with clinical and pathological parameters were investigated. ProNGF staining intensity (defined by the median h-score) was significantly higher in lymph node metastases (h-score 94, interquartile range (IQR) 50–147) than in corresponding primary tumours (57, IQR 42–84) (p = 0.002). There was a correlation between proNGF expression in primary tumours and corresponding metastases, where there was a 0.68 (95% CI 0 to 1.2) increase in metastatic tumour h-score for each unit increase in the primary tumour h-score. However, larger tumours (both primary and metastatic) had lower proNGF expression. In a multivariate model, proNGF expression in nodal metastases was negatively correlated with lateral neck disease and being male. In conclusion, ProNGF is expressed in locoregional metastases of thyroid cancer and is higher in lymph node metastases than in primary tumours, but is not associated with high-risk clinical features.
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spelling pubmed-69291172019-12-26 The Precursor for Nerve Growth Factor (proNGF) in Thyroid Cancer Lymph Node Metastases: Correlation with Primary Tumour and Pathological Variables Rowe, Christopher W. Dill, Tony Faulkner, Sam Gedye, Craig Paul, Jonathan W. Tolosa, Jorge M. Jones, Mark King, Simon Smith, Roger Hondermarck, Hubert Int J Mol Sci Article Metastases in thyroid cancer are associated with aggressive disease and increased patient morbidity, but the factors driving metastatic progression are unclear. The precursor for nerve growth factor (proNGF) is increased in primary thyroid cancers, but its expression or significance in metastases is not known. In this study, we analysed the expression of proNGF in a retrospective cohort of thyroid cancer lymph node metastases (n = 56), linked with corresponding primary tumours, by automated immunohistochemistry and digital quantification. Potential associations of proNGF immunostaining with clinical and pathological parameters were investigated. ProNGF staining intensity (defined by the median h-score) was significantly higher in lymph node metastases (h-score 94, interquartile range (IQR) 50–147) than in corresponding primary tumours (57, IQR 42–84) (p = 0.002). There was a correlation between proNGF expression in primary tumours and corresponding metastases, where there was a 0.68 (95% CI 0 to 1.2) increase in metastatic tumour h-score for each unit increase in the primary tumour h-score. However, larger tumours (both primary and metastatic) had lower proNGF expression. In a multivariate model, proNGF expression in nodal metastases was negatively correlated with lateral neck disease and being male. In conclusion, ProNGF is expressed in locoregional metastases of thyroid cancer and is higher in lymph node metastases than in primary tumours, but is not associated with high-risk clinical features. MDPI 2019-11-25 /pmc/articles/PMC6929117/ /pubmed/31775361 http://dx.doi.org/10.3390/ijms20235924 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rowe, Christopher W.
Dill, Tony
Faulkner, Sam
Gedye, Craig
Paul, Jonathan W.
Tolosa, Jorge M.
Jones, Mark
King, Simon
Smith, Roger
Hondermarck, Hubert
The Precursor for Nerve Growth Factor (proNGF) in Thyroid Cancer Lymph Node Metastases: Correlation with Primary Tumour and Pathological Variables
title The Precursor for Nerve Growth Factor (proNGF) in Thyroid Cancer Lymph Node Metastases: Correlation with Primary Tumour and Pathological Variables
title_full The Precursor for Nerve Growth Factor (proNGF) in Thyroid Cancer Lymph Node Metastases: Correlation with Primary Tumour and Pathological Variables
title_fullStr The Precursor for Nerve Growth Factor (proNGF) in Thyroid Cancer Lymph Node Metastases: Correlation with Primary Tumour and Pathological Variables
title_full_unstemmed The Precursor for Nerve Growth Factor (proNGF) in Thyroid Cancer Lymph Node Metastases: Correlation with Primary Tumour and Pathological Variables
title_short The Precursor for Nerve Growth Factor (proNGF) in Thyroid Cancer Lymph Node Metastases: Correlation with Primary Tumour and Pathological Variables
title_sort precursor for nerve growth factor (prongf) in thyroid cancer lymph node metastases: correlation with primary tumour and pathological variables
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929117/
https://www.ncbi.nlm.nih.gov/pubmed/31775361
http://dx.doi.org/10.3390/ijms20235924
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