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Nucleoplasmic Reticulum Formation in Human Endometrial Cells is Steroid Hormone Responsive and Recruits Nascent Components

The nuclei of cells may exhibit invaginations of the nuclear envelope under a variety of conditions. These invaginations form a branched network termed the nucleoplasmic reticulum (NR), which may be found in cells in pathological and physiological conditions. While an extensive NR is a hallmark of c...

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Autores principales: Pytowski, Lior, Drozdz, Marek M., Jiang, Haibo, Hernandez, Zayra, Kumar, Kurun, Knott, Emily, Vaux, David J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929123/
https://www.ncbi.nlm.nih.gov/pubmed/31757079
http://dx.doi.org/10.3390/ijms20235839
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author Pytowski, Lior
Drozdz, Marek M.
Jiang, Haibo
Hernandez, Zayra
Kumar, Kurun
Knott, Emily
Vaux, David J.
author_facet Pytowski, Lior
Drozdz, Marek M.
Jiang, Haibo
Hernandez, Zayra
Kumar, Kurun
Knott, Emily
Vaux, David J.
author_sort Pytowski, Lior
collection PubMed
description The nuclei of cells may exhibit invaginations of the nuclear envelope under a variety of conditions. These invaginations form a branched network termed the nucleoplasmic reticulum (NR), which may be found in cells in pathological and physiological conditions. While an extensive NR is a hallmark of cellular senescence and shows associations with some cancers, very little is known about the formation of NR in physiological conditions, despite the presence of extensive nuclear invaginations in some cell types such as endometrial cells. Here we show that in these cells the NR is formed in response to reproductive hormones. We demonstrate that oestrogen and progesterone are sufficient to induce NR formation and that this process is reversible without cell division upon removal of the hormonal stimulus. Nascent lamins and phospholipids are incorporated into the invaginations suggesting that there is a dedicated machinery for its formation. The induction of NR in endometrial cells offers a new model to study NR formation and function in physiological conditions.
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spelling pubmed-69291232019-12-26 Nucleoplasmic Reticulum Formation in Human Endometrial Cells is Steroid Hormone Responsive and Recruits Nascent Components Pytowski, Lior Drozdz, Marek M. Jiang, Haibo Hernandez, Zayra Kumar, Kurun Knott, Emily Vaux, David J. Int J Mol Sci Article The nuclei of cells may exhibit invaginations of the nuclear envelope under a variety of conditions. These invaginations form a branched network termed the nucleoplasmic reticulum (NR), which may be found in cells in pathological and physiological conditions. While an extensive NR is a hallmark of cellular senescence and shows associations with some cancers, very little is known about the formation of NR in physiological conditions, despite the presence of extensive nuclear invaginations in some cell types such as endometrial cells. Here we show that in these cells the NR is formed in response to reproductive hormones. We demonstrate that oestrogen and progesterone are sufficient to induce NR formation and that this process is reversible without cell division upon removal of the hormonal stimulus. Nascent lamins and phospholipids are incorporated into the invaginations suggesting that there is a dedicated machinery for its formation. The induction of NR in endometrial cells offers a new model to study NR formation and function in physiological conditions. MDPI 2019-11-20 /pmc/articles/PMC6929123/ /pubmed/31757079 http://dx.doi.org/10.3390/ijms20235839 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pytowski, Lior
Drozdz, Marek M.
Jiang, Haibo
Hernandez, Zayra
Kumar, Kurun
Knott, Emily
Vaux, David J.
Nucleoplasmic Reticulum Formation in Human Endometrial Cells is Steroid Hormone Responsive and Recruits Nascent Components
title Nucleoplasmic Reticulum Formation in Human Endometrial Cells is Steroid Hormone Responsive and Recruits Nascent Components
title_full Nucleoplasmic Reticulum Formation in Human Endometrial Cells is Steroid Hormone Responsive and Recruits Nascent Components
title_fullStr Nucleoplasmic Reticulum Formation in Human Endometrial Cells is Steroid Hormone Responsive and Recruits Nascent Components
title_full_unstemmed Nucleoplasmic Reticulum Formation in Human Endometrial Cells is Steroid Hormone Responsive and Recruits Nascent Components
title_short Nucleoplasmic Reticulum Formation in Human Endometrial Cells is Steroid Hormone Responsive and Recruits Nascent Components
title_sort nucleoplasmic reticulum formation in human endometrial cells is steroid hormone responsive and recruits nascent components
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929123/
https://www.ncbi.nlm.nih.gov/pubmed/31757079
http://dx.doi.org/10.3390/ijms20235839
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