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Plasma Levels of snoRNAs are Associated with Platelet Activation in Patients with Peripheral Artery Disease

In addition to supervised walking therapy, antithrombotic therapy and the management of risk factors, the treatment of peripheral artery disease (PAD) is limited to endovascular and surgical interventions, i.e., angioplasty with stent implantation and bypass surgery, respectively. Both are associate...

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Autores principales: Nossent, Anne Yaël, Ektefaie, Neda, Wojta, Johann, Eichelberger, Beate, Kopp, Christoph, Panzer, Simon, Gremmel, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929168/
https://www.ncbi.nlm.nih.gov/pubmed/31783567
http://dx.doi.org/10.3390/ijms20235975
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author Nossent, Anne Yaël
Ektefaie, Neda
Wojta, Johann
Eichelberger, Beate
Kopp, Christoph
Panzer, Simon
Gremmel, Thomas
author_facet Nossent, Anne Yaël
Ektefaie, Neda
Wojta, Johann
Eichelberger, Beate
Kopp, Christoph
Panzer, Simon
Gremmel, Thomas
author_sort Nossent, Anne Yaël
collection PubMed
description In addition to supervised walking therapy, antithrombotic therapy and the management of risk factors, the treatment of peripheral artery disease (PAD) is limited to endovascular and surgical interventions, i.e., angioplasty with stent implantation and bypass surgery, respectively. Both are associated with a high restenosis rate. Furthermore, patients with PAD often suffer atherothrombotic events like myocardial infarction, transient ischemic attacks or stroke. Small ribonucleic acids (RNAs) have proven reliable biomarkers because of their remarkable stability. Small nucleolar RNAs (snoRNAs) guide modifications to small nuclear RNAs and ribosomal RNAs, enabling protein synthesis. In the current study, we measured four snoRNAs in 104 consecutive PAD patients who underwent elective infrainguinal angioplasty with stent implantation. We selected snoRNAs that showed significant overexpression in the plasma of end-stage PAD patients in a previous study. All four snoRNAs are transcribed from the 14q32 locus, which is strongly linked to human cardiovascular disease, including PAD and restenosis. We showed that the four selected 14q32 snoRNAs were abundantly expressed in the plasma of PAD patients. The plasma levels of these snoRNAs were not directly associated with target vessel restenosis, however, levels of SNORD113.2 and SNORD114.1 were strongly linked to platelet activation, which is an important determinant of long-term outcome, in PAD, and in cardiovascular disease in general.
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spelling pubmed-69291682019-12-26 Plasma Levels of snoRNAs are Associated with Platelet Activation in Patients with Peripheral Artery Disease Nossent, Anne Yaël Ektefaie, Neda Wojta, Johann Eichelberger, Beate Kopp, Christoph Panzer, Simon Gremmel, Thomas Int J Mol Sci Article In addition to supervised walking therapy, antithrombotic therapy and the management of risk factors, the treatment of peripheral artery disease (PAD) is limited to endovascular and surgical interventions, i.e., angioplasty with stent implantation and bypass surgery, respectively. Both are associated with a high restenosis rate. Furthermore, patients with PAD often suffer atherothrombotic events like myocardial infarction, transient ischemic attacks or stroke. Small ribonucleic acids (RNAs) have proven reliable biomarkers because of their remarkable stability. Small nucleolar RNAs (snoRNAs) guide modifications to small nuclear RNAs and ribosomal RNAs, enabling protein synthesis. In the current study, we measured four snoRNAs in 104 consecutive PAD patients who underwent elective infrainguinal angioplasty with stent implantation. We selected snoRNAs that showed significant overexpression in the plasma of end-stage PAD patients in a previous study. All four snoRNAs are transcribed from the 14q32 locus, which is strongly linked to human cardiovascular disease, including PAD and restenosis. We showed that the four selected 14q32 snoRNAs were abundantly expressed in the plasma of PAD patients. The plasma levels of these snoRNAs were not directly associated with target vessel restenosis, however, levels of SNORD113.2 and SNORD114.1 were strongly linked to platelet activation, which is an important determinant of long-term outcome, in PAD, and in cardiovascular disease in general. MDPI 2019-11-27 /pmc/articles/PMC6929168/ /pubmed/31783567 http://dx.doi.org/10.3390/ijms20235975 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nossent, Anne Yaël
Ektefaie, Neda
Wojta, Johann
Eichelberger, Beate
Kopp, Christoph
Panzer, Simon
Gremmel, Thomas
Plasma Levels of snoRNAs are Associated with Platelet Activation in Patients with Peripheral Artery Disease
title Plasma Levels of snoRNAs are Associated with Platelet Activation in Patients with Peripheral Artery Disease
title_full Plasma Levels of snoRNAs are Associated with Platelet Activation in Patients with Peripheral Artery Disease
title_fullStr Plasma Levels of snoRNAs are Associated with Platelet Activation in Patients with Peripheral Artery Disease
title_full_unstemmed Plasma Levels of snoRNAs are Associated with Platelet Activation in Patients with Peripheral Artery Disease
title_short Plasma Levels of snoRNAs are Associated with Platelet Activation in Patients with Peripheral Artery Disease
title_sort plasma levels of snornas are associated with platelet activation in patients with peripheral artery disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6929168/
https://www.ncbi.nlm.nih.gov/pubmed/31783567
http://dx.doi.org/10.3390/ijms20235975
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